湖北七姊妹山自然保护区兽类资源初步研究

对七姊妹山自然保护区的兽类资源进行了考察，报道兽类共58种，隶属于8目22科，保护区有国家重点保护兽类42种：Ⅰ级3种，Ⅱ级12种，有益的或有重要经济、科研价值的兽类27种；有CITES附录的兽类21种，对兽类的区系组成、种群数量和保护现状进行了研究，并提出了保护对策。     

Using Toad Oocytes as an Ultrasensitive System for Estimating the Biological Activity of Trichosanthin

A sensitive bioassay for estimating the activity of Trichosanthin was developed by using intraoocyte microinjection as the technique and germinal vescicle breakdown as a marker. The GVBD inhibitory activity assay was million-fold more sensitive than the routinely used abortifacient activity assay. The detectable amount of Trichosanthin ranged from 0.5 to 100 pg. Since the least detectable dose was 0.5 pg, it made the assay suitable to analyse the microquantity of pure Trichosanthin used for studying the structure and function of the drug.     

A FACTOR FROM GRANULOSA CELLS CAN STIMULATE OOCYTE TISSUE PLASMINOGEN ACTIVATOR ACTIVITY

Several studies indicate that substances synthesized by granulosa cells are capableof regulating oocyte activity. We have studied the effect of factors synthesized by gran-ulosa cells on tPA activity of denuded oocytes using a co- culture system. The resultsshow that an FSH- dependent factor(s) synthesized by granulosa cells (but not by theca-interstitial cells) is capable of stimulating tPA activity of denuded oocytes. This findingis important for understanding hormonal regulation of oocyte tPA activity by mediatorssynthesized in granulosa cells.     

黄石坳保护区的兽类初步研究

2002年5—7月对广东和平县黄石坳保护区的兽类进行了调查，共记录到兽类6目15科31种，东洋型21种占67．7％；南中国型5种占16．1％；古北型3种占9．7％；季风型和喜马拉雅山型各1种占3．2％．其中国家I级保护动物1种；Ⅱ级保护动物5种；三有动物10种，说明该地区物种珍稀程度较高．翼手类共记录到12种，占所有物种总数的38．7％；啮齿类只有3种，占种数的9．6％，可能与捕获的季节等有关．     

Roles of protein kinase C in oocyte meiotic maturation and fertilization

Protein kinase C (PKC) is a superfamily of Ser/Thr protein kinases that is distributed widely in eukaryotes. It plays key regulatory roles at multiple steps of oocyte meiotic maturation and fertilization. During the process of meiotic maturation, the activation of PKC in cumulus cells stimulates meiotic maturation, whereas the activation of PKC in oocytes results in the inhibition of germinal vesicle breakdown. PKC activity increases following the meiotic maturation, and decreases at the transition of metaphase/anaphase in meiosis I, so as to facilitate the release of the first polar body and the entry of meiosis II. In fertilization of mammalian oocytes, PKC may act as one of the downstream targets of Ca2+ to stimulate the cortical granule exocytosis, release the oocytes from MII arrest and to induce pronucleus formation. PKC is also involved in the regulation of maturation promoting factor (MPF) and mitogen-activated protein kinase (MAPK). Several PKC isoforms have been identified in mammalian oocytes, and there is evidence showing that classical PKCs may be the principal mediator of oocyte cortical reaction.     

金佛山自然保护区兽类资源初步调查

对四川省南川县境内金佛山省级自然保护区的兽类资源进行初步设想，报道兽类共７０种，分属８目２４科，其中属示洋界的有３９种，古北界６种，广布种２５种，属横断山脉－喜马拉雅山脉特产的５种。国家Ⅰ级重点保护动物４种，Ⅱ级１１种，省级保护５种。     

Function and interaction of maturation-promoting factor and mitogen-activated protein kinase during meiotic maturation and fertilization of oocyte

Mitogen-activated protein kinase (MAP kinase) cascade and maturation-promoting factor (MPF) play very important roles during meiotic maturation and fertilization of oocyte. Interaction between MAP kinase and MPF influences meiotic maturation and fertilization of oocyte throughout the animal kingdom, including stimulation of germinal vesicle breakdown (GVBD), suppression of DNA replication, control of meiotic chromosome segregation, maintenance of metaphase II arrest, and resumption and completion of second meiosis. This review focuses on the function and interaction of MAP kinase and MPF during meiotic maturation and fertilization of oocyte.     

湘西更新世哺乳动物化石痕迹考察研究

目的 考察湘西土家族苗族自治州古丈县断龙乡猛虎洞更新世哺乳动物群骨化石断裂痕迹的状态与原因,探索湘西地区古人类活动的遗迹.方法 观测哺乳动物群骨化石断裂痕迹剖面的形态结构,以区别食肉类动物群的咬痕与人类打造的印迹.结果 经40件东方剑齿象、中国犀、鹿等残骨化石标本断裂痕迹剖面的观测,发现有部分骨化石断裂剖面圆钝,似啮齿动物的咬痕,但大部分骨化石断端剖面呈锥形切削改造,有的双面削刮呈锐利器型,有的存在明显打击或铲刮的 “印记”,初步认为属早期人类制作骨器的遗迹.结论 结合地理环境和地学结构分析,猛虎洞可能属晚更新世(10～5)万年前旧石器人类穴居的营地或系同生代古脊椎动物的遗址.