国外图书馆科研支持服务研究

介绍了图书馆科研支持服务的背景,分析了国外图书馆科研支持服务的种类,举例说明了国外图书馆科研支持服务的具体实践.     

高校图书馆信息工作的现状、困扰及对策

当前，高校图书馆信息服务观念没有真正树立，机构不健全，人员不足，经费紧张以及信息资源的极大浪费使信息工作面临困境。要摆脱这种困境，必须提高相关人员的信息服务意识，通过成立专门的信息服务委员会，提高信息服务人员的素质，向社会广开信息服务市场，拓宽筹措经费渠道，促进信息资源开发和信息资源更新的良性循环     

Dendrimers and combinatorial chemistry—tools for fluorescent enhancement in protease assays

FRET based systems are some of the best methods available to detect and monitor proteolytic activity. To enhance fluorescent signals and hence assay sensitivity, two different systems were developed using two different dendrimeric constructs. In the first case, a triple branched dendrimer bearing three dansyl groups was used to enhance assay sensitivity and showed a significant enhancement of fluorescence following enzymatic cleavage. In another example, a tris-fluorescein probe, that undergoes self-quenching, was utilized in a combinatorial library synthesis to map the substrate specificity of proteases.     

Structural aspects of antibody-antigen interaction revealed through small random peptide libraries

Summary Two small random peptide libraries, one composed of 4550 dodecapeptides and one of 8000 tripeptides, were synthesized in newly developed credit-card format miniPEPSCAN cards (miniPEPSCAN libraries). Each peptide was synthesized in a discrete well (455 peptides/card). The two miniPEPSCAN libraries were screened with three different monoclonal antibodies (Mabs). Two other random peptide libraries, expressed on the wall of bacteria (recombinant libraries) and composed of 10⁷ hexa- and octapeptides, were screened with the same three Mabs. The aim of this study was to compare the amino acid sequence of peptides selected from small and large pools of random peptides and, in this way, investigate the potential of small random peptide libraries. The screening of the two miniPEPSCAN libraries resulted in the identification of a surprisingly large number of antibody-binding peptides, while the screening of the large recombinant libraries, using the same Mabs, resulted in the identification of only a small number of peptides. The large number of peptides derived from the small random peptide libraries allowed the determination of consensus sequences. These consensus sequences could be related to small linear and nonlinear parts of the respective epitopes. The small number of peptides derived from the large random peptide libraries could only be related to linear epitopes that were previously mapped using small libraries of overlapping peptides covering the antigenic protein. Thus, with respect to the cost and speed of identifying peptides that resemble linear and nonlinear parts of epitopes, small diversity libraries based on synthetic peptides appear to be superior to large diversity libraries based on expression systems.Abbreviations ABTS 2,2-azino-di-3-ethylbenzthiazoline sulfonate - EGF epidermal growth factor - Mab monoclonal antibody - OD_ccd optical density obtained using CCD camera - RAMPO rabbit-antimouse peroxidase - SDS sodium dodecylsulfate - TGEV transmissible gastroenteritis virus     

化合物库的组合技巧和组合合成

从化合物库的组合技巧和组合合成方法两个方面探讨了组合化学及其在药物开发中的应用。     

试论现代图书馆的人文精神

通过对图书馆人文精神内涵及发展的阐述，指出了网络环境下图书馆的人文精神的严重缺失问题，从而提出了现阶段图书馆倡导人文精神的必要性及其具体实践途径。     

高校数字图书馆的文献资源建设

文章概述了数字图书馆的基本特征、主要模式以及高校图书馆文献资源网络化的必然性,提出了数字图书馆文献资源建设的策略.     

高校图书馆古籍保护工作初探

古籍是中华民族的宝贵遗产,加强保护和管理措施,是高校图书馆的一项重要工作,也是高校图书馆科学管理的重要体现。