To study the relations between male infertility and centrosome protein (centrin) and the functions of centrin in spermatogenesis,
the matured spermatozoa of 10 normal male people and 18 male infertility patients were stained by immunofluorescence labeling
antibody against centrin. The results showed that two fluorescence signal dots appeared in the normal male spermatozoa and
were located at the base of flagellum. They are proximal centriole and distal centriole. However, in some spermatozoa of the
male infertility, centrin protein was located abnormally at the base of flagellum and its staining signals were spread, the
normal proximal and distal centrioles were confused and could not be recognized separately. These results suggest that abnormality
of centrosome protein may be related to male infertility. This discovery may be used as a marker of abnormal sperm and male
infertility. 相似文献
Producing meiosis‐competent germ cells (GCs) from embryonic stem cells (ESCs) is essential for developing advanced therapies for infertility. Here, a novel approach is presented for generation of GCs from ESCs. In this regard, microparticles (MPs) have been developed from alginate sulfate loaded with bone morphogenetic protein 4 (BMP4). The results here show that BMP4 release from alginate sulfate MPs is significantly retarded by the sulfated groups compared to neat alginate. Then, BMP4‐laden MPs are incorporated within the aggregates during differentiation of GCs from ESCs. It is observed that BMP4‐laden MPs increase GC differentiation from ESCs at least twofold compared to the conventional soluble delivery method. Interestingly, following meiosis induction, Dazl , an intrinsic factor that enables GCs to enter meiosis, and two essential meiosis genes (Stra8 and Smc1b ) are upregulated significantly in MP‐induced aggregates compared to aggregates, which are formed by the conventional method. Together, these data show that controlled delivery of BMP4 during ESC differentiation into GC establish meiosis‐competent GCs which can serve as an attractive GC source for reproductive medicine.
“试管婴儿之父”罗伯特·爱德华兹(Robert G. Edwards)获得2010年度诺贝尔生理学或医学奖,以表彰他在体外受精(IVF)技术方面的杰出贡献。早在20世纪50年代,爱德华兹便提出通过体外受精治疗人类不育的设想:即从女性卵巢内取出卵子,与精子在细胞培养皿中完成受精,然后再移植回母体子宫内发育。他们经过漫长而艰辛的努力实现了这一目标,1978年世界第一例“试管婴儿”出生。这项技术给全世界大约10%的不育症夫妇带来希望。一门新的医学领域(辅助生殖技术)由此诞生,开启现代医学的新世界。 相似文献