HPLC法测定小儿导赤片中大黄素含量

目的:建立用高效液相色谱(HPLC)法测定小儿导赤片中大黄素的含量.方法:采用HPLC法,色谱柱为Agilent ZORBAXSB-C18(4.6mm×150 mm,5μm),流动相为甲醇-0.1%磷酸水溶液(80:20),流速为1.0 ml/min,柱温为30℃,检测波长为254nm.结果:大黄素在0.04～0.4μg(r=0.999 9)范围内有良好的线性关系.大黄素平均回收率分别为98.25%.结论:该方法操作简便、快速、结果准确可靠.     

反相高效液相色谱法同时测定决明子中芦荟大黄素和大黄素

用反相高效液相色谱法分离并测定了决明子中芦荟大黄素和大黄素，建立了该中药中芦荟大黄素、大黄素分离、测定的色谱方法。色谱条件：ＯＤＳ柱，甲醇－水（８０∶２０Ｖ／Ｖ）为流动相，检测波长２２３ｎｍ。本研究为决明子的质量评价提供了科学依据。     

大黄素的吸附伏安行为及定量测定

大黄素在1％硼砂底液中，有一灵敏的吸附伏安还原峰．峰电位为-0．86V(vs.Ag／AgCl)．在电位-0．3V下预富集，采用微分脉冲吸附溶出伏安法测定，其检测限可达8．0×10^-10mol／L，大黄素在1×10^-8～1×10^-6mol／L浓度范围内．峰电流与浓度呈线性关系此法简便、快速、可靠．     

Overview of the Biological Activity of Anthraquinons and Flavanoids of the Plant Rumex Species

Rumex confertus belongs to the genus Rumex and is classified as an invasive parasitic plant in agriculture. Despite other Rumex species being widely used in herbal medicine due to their antimicrobial, antioxidant, antitumor, and anti-inflammatory effects, there are almost no information about the potential of Rumex confertus for the treatment of various diseases. In this review we analyzed scientific articles revealing properties of Rumex plant’s substances against cancer, diabetes, pathogenic bacterial invasions, viruses, inflammation, and oxidative stress for the past 20 years. Compounds dominating in each composition of solvents for extraction were discussed, and common thin layer chromatography(TLC) and high performance liquid chromatography(HPLC) methods for efficient separation of the plant’s extract are included. Physico-chemical properties such as solubility, hydrophobicity (Log P), pKa of flavonoids, anthraquinones, and other derivatives are very important for modeling of pharmacokinetic and pharmacodynamics. An overview of clinical studies for abounded selected substances of Rumex species is presented.     

Divergent and Overlapping Roles for Selected Phytochemicals in the Regulation of Pathological Cardiac Hypertrophy

Plant-based foods, like fruits, vegetables, whole grains, legumes, nuts, seeds and other foodstuffs, have been deemed as heart healthy. The chemicals within these plant-based foods, i.e., phytochemicals, are credited with protecting the heart. However, the mechanistic actions of phytochemicals, which prevent clinical endpoints, such as pathological cardiac hypertrophy, are still being elucidated. We sought to characterize the overlapping and divergent mechanisms by which 18 selected phytochemicals prevent phenylephrine- and phorbol 12-myristate 13-acetate-mediated cardiomyocyte enlargement. Of the tested 18 compounds, six attenuated PE- and PMA-mediated enlargement of neonatal rat ventricular myocytes. Cell viability assays showed that apigenin, baicalein, berberine hydrochloride, emodin, luteolin and quercetin dihydrate did not reduce cell size through cytotoxicity. Four of the six phytochemicals, apigenin, baicalein, berberine hydrochloride and emodin, robustly inhibited stress-induced hypertrophy and were analyzed further against intracellular signaling and genome-wide changes in mRNA expression. The four phytochemicals differentially regulated mitogen-activated protein kinases and protein kinase D. RNA-sequencing further showed divergence in gene regulation, while pathway analysis demonstrated overlap in the regulation of inflammatory pathways. Combined, this study provided a comprehensive analysis of cardioprotective phytochemicals. These data highlight two defining observations: (1) that these compounds predominantly target divergent gene pathways within cardiac myocytes and (2) that regulation of overlapping signaling and gene pathways may be of particular importance for the anti-hypertrophic actions of these phytochemicals. Despite these new findings, future works investigating rodent models of heart failure are still needed to understand the roles for these compounds in the heart.     

Analysis of the pharmacokinetics and metabolism of aloe‐emodin following intravenous and oral administrations in rats

Aloe‐emodin, a natural polyphenolic anthraquinone, has shown various beneficial bioactivities in vitro. The aim of this study was to investigate the pharmacokinetics and metabolism of aloe‐emodin. Aloe‐emodin was intravenously and orally administered to rats. The concentrations of aloe‐emodin and rhein, a metabolite of aloe‐emodin, were determined by HPLC method prior to and after hydrolysis with β‐glucuronidase and sulfatase/β‐glucuronidase. The results showed that the systemic exposures of aloe‐emodin and its metabolites were ranked as aloe‐emodin glucuronides (G) > rhein sulfates (S) > aloe‐emodin > rhein and rhein G when aloe‐emodin was given intravenously. In contrast, when aloe‐emodin was administered orally, the parent form of aloe‐emodin was not absorbed per se, and the systemic exposures of its metabolites were ranked as aloe‐emodin G > rhein G > rhein. In conclusion, the metabolites of aloe‐emodin are more important than the parent form for the bioactivities in vivo. Copyright © 2016 John Wiley & Sons, Ltd.     

虎杖中大黄素的分离及抗菌活性的初步研究

采用pH梯度法提取虎杖中的大黄素，波谱分析鉴定其结构，然后用滤纸扩散法及薄层色谱自显影生物技术研究其对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、绿脓杆菌、草分枝杆菌的抗菌效果．结果显示：大黄素对这5种细菌均有抑制作用，其在薄层色谱板上最小的抑菌量分别是：金黄色葡萄球菌为2．35μg；大肠杆菌为75μg；枯草芽孢杆菌为4．7μg；绿脓杆菌为4．7μg；草分枝杆菌为18．8μg．     

高速逆流色谱分离制备大黄化学成分

采用pH-区带精制逆流色谱(pH-ZRCCC)与常规高速逆流色谱(HSCCC)结合技术分离制备大黄粗提物中的化学成分。首先采用pH-ZRCCC分离大黄粗提物,溶剂体系为石油醚-乙酸乙酯-甲醇-水(3:7:4:6,V/V),上相加TFA(10 mmol/L)作为固定相,下相加Na OH(15 mmol/L)作为流动相。从1.3 g粗提物中得到140 mg桂皮酸(纯度为96.1%)、130 mg大黄酸(纯度为92.5%)和560 mg尾吹物。称取260 mg该尾吹物采用常规HSCCC的方法进一步分离,溶剂体系为石油醚-乙酸乙酯-甲醇-水(7:3:7:4,V/V),得到约98 mg大黄素(纯度为96.5%)和60 mg芦荟大黄素(纯度为94.6%)。用HPLC检测纯度,用ESI-MS和1HNMR鉴定结构。研究结果表明pH-ZRCCC与HSCCC结合是分离大黄中化合物的一种有效方法。     

Electrochemical Behavior of Herbal Antitumor Drug Aloe‐Emodin at Carbon‐Coated Nickel Magnetic Nanoparticles Modified Glassy Carbon Electrode

The electrochemical behavior of aloe‐emodin (AE), an important herbal antitumor drug, was investigated at a carbon‐coated nickel magnetic nanoparticles modified glassy carbon electrode (CNN/GCE). A couple of well‐defined redox peaks was obtained. Some electrochemical parameters of AE at a CNN/GCE, such as the charge number, exchange current density, standard heterogeneous rate constant, were measured. The square wave voltammetry (SWV) response of AE was linear with the concentration over two concentration intervals viz. 6.24×10^?9?1.13×10^?6 M and 1.13×10^?6?1.23×10^?5 M, with a detection limit of 2.08 nM. A fast, simple and sensitive detection and analysis of AE was developed.     

蒽醌类亲脂性阳离子的合成及抗癌活性

合成了7个大黄素季铵盐、2个芦荟大黄素季铵盐、1个水溶性大黄素季铵盐和1个α-萘酚醌苯基甲烷季铵盐化合物,并测试了其抗癌活性.含有1条长碳链的大黄素季铵盐的抗癌活性很低,但是含有2条长碳链的大黄素和芦荟大黄素季铵盐的抗癌活性较好.用亲水性的长链替代季铵盐中亲脂性的长碳链会导致大黄素季铵盐失去抗癌活性.α-萘酚醌苯基甲烷季铵盐显示了中等的抗癌活性,表明在具有电子传递能力的分子中引入亲脂性的长碳链季铵盐可以增加其抗癌活性.