一种高效纯化血清淀粉样物质A(SAA)的方法

通过密度梯度超速离心、脱脂从急性相胸水中分离得到含有血清淀粉样物质A（Serum AmyloidA,SAA）的高密度脂蛋白（HDL），再通过两次Sephacry1S-200层析纯化SAA蛋白。经SDS-PAGE电泳和Western Blotting检验证实得到的SAA蛋白纯度高，可作为抗原免疫动物制备抗体，方法可用于从胸水中大量制备SAA。     

基于改进模拟退火算法的推动式生产-配送协调优化

为减小物资生产与配送不协调造成的成本及生产资源浪费,建立了考虑推动式生产调度的物资配送优化模型,并针对标准模拟退火算法受随机因素影响易陷入局部最优的缺点,设计带有回火与缓冷操作的改进模拟退火算法对模型求解,确定了优化的车辆配送路线以及物资生产计划。对比实验结果表明：相对于单纯的物资配送优化模型,考虑推动式生产调度的配送优化模型,能够有效减小物资滞留时间以及配送延误成本;相较于标准模拟退火算法,改进算法搜索到了更优解,且计算结果的标准差减小了93.42%,稳定性更好;同时,改进模拟退火算法具有较低的偏差率,在中小规模算例中求解质量较高,平均偏差率在0.5%以内。     

斑马鱼SAA基因克隆及表达分析

利用巢式PCR(nest PCR)的方法克隆斑马鱼血清淀粉样蛋白A基因,并进行生物信息学分析.实时荧光定量PCR结果显示:SAA在斑马鱼胚胎发育时期的表达量呈现明显的“增长—稳定—增长”趋势.成鱼组织分布中,SAA在11个斑马鱼组织中均能检测到,在心脏和肝脏中相对高表达.     

基于ARM9的机顶盒方案设计

将菲利浦公司的模拟电视信号采集处理芯片TDA8275、TDA8290和该公司的解码芯片SAA7133,同三星公司的具有PCI接口的ARM9核S3C2510A相结合,设计出具有存储电视节目和上网功能的机顶盒,具有一定的应用价值和市场前景.     

Numerical simulation of the radar echo from a ship in ocean clutter

A comprehensive approach which combines the generalized forward-backward method (GFBM) with the spectral acceleration algorithm (SAA) is developed. The angular radar echo from a rough ocean surface with a ship present, and with the transverse electric (TE) and transverse magnetic (TM) components incidencing at low grazing angle, is numerically simulated; and its functional dependences upon the polarization, observation angle, sea surface wind speed, ship location and other parameters are analyzed.     

Solving multistage asset investment problems by the sample average approximation method

The vast size of real world stochastic programming instances requires sampling to make them practically solvable. In this paper we extend the understanding of how sampling affects the solution quality of multistage stochastic programming problems. We present a new heuristic for determining good feasible solutions for a multistage decision problem. For power and log-utility functions we address the question of how tree structures, number of stages, number of outcomes and number of assets affect the solution quality. We also present a new method for evaluating the quality of first stage decisions.     

Application of thiophilic chromatography to deplete serum immunoglobulins in sample preparation for bidimensional electrophoresis

Serum is a typical sample for non-invasive studies in clinical research. Its proteome characterization is challenging, since requires extensive protein depletion. Methods used nowadays for removal of high-abundance proteins are expensive or show quite often a low loading capacity, which has strong repercussions on the number of samples and replicates per analysis.In order to deplete immunoglobulins (Igs) and albumin (HSA) from 1 mL serum samples, we have developed a protocol based on a combination of thiophilic chromatography, not previously used in clinical proteomics, and a HSA-specific resin. Ig/HSA-depleted samples, immunoglobulinome and albuminone were analyzed by 2-DE. Thiophilic chromatography, coupled with HSA-depletion, allows a good 2-DE resolution as well as the visualization of new spots. Moreover, it yields enough protein to evaluate technical variability and facilitate subsequent protein identification. To validate the protocol, we carried out a preliminary comparative study between triplicate Igs/HSA-depleted serum samples from healthy control individuals and recently diagnosed/untreated rheumatoid arthritis (RA) patients. RA patients showed several acute phase proteins, as well as additional serum proteins, differentially and significantly regulated.Therefore, thiophilic chromatography can be used as an efficient and economical method in 2-DE to deplete immunoglobulins from large human serum samples before a more extensive fractioning.     

分形粗糙面双站散射的快速前后向迭代法数值模拟

为模拟复杂分形表面特别是在低掠角入射条件下的双站散射,发展了一种结合前后向迭代方法(FBM)与谱加速算法(SAA)快速求解散射场的Monte Carlo数值方法,计算了在TE,TM锥形波入射在一维分形导体粗糙面的双站散射以及有规则异物存在时的双站散射,讨论了分形粗糙面双站散射的角度性分布与其分数维的关系. 关键词： GFBM/SAA 分形粗糙面 双站散射     

低聚型表面活性剂的研究(二)——RT_mG_n的合成及性能研究

本文合成了以缩水甘油乙酸酯和四氢呋喃为单休,长链脂肪醇为链转移剂的共聚物,对该共聚物进行干法醇解,制得了聚(氧四亚甲基)聚(甘油基)烷基醚类非离子型表面活性剂,记为RT_mG_n。对RT_mG_n进行了结构分析和性能研究。实验结果表明:RT_mG_n聚甘油醚类非离子型表面活性剂具有良好的化学稳定性和表面化学性质,无毒、无刺激,可作为乳化剂应用于化妆品中。     

基于DSP的视频编解码器的控制

以TMS320C6416为核心,实现视频图像的实时采集与处理。利用DSP的GPIO口模拟I2C总线实现对视频编解码器的寄存器控制。系统具有结构简单、配置灵活、实时性高、通用性强的优点。