底栖无脊椎动物群落与生物监测

利用无脊椎动物群落作为生物监测的条件，将具有潜在胁迫的系统与未受干扰的基准区群落进行比较，以评价测试系统的状态．着重讨论了基准条件法建模过程及结果的分析方法，并提出了其应用前景．     

Cleaning requirements for silica‐coated sensors used in optical waveguide lightmode spectroscopy

Optical waveguide lightmode spectroscopy (OWLS), based on the incoupling of laser light into a waveguide sensor by an optical grating, allows for the in situ measurement of protein adsorption. Few reports have described cleaning methods for the surfaces of such sensors, and in this investigation, we compare common methods for cleaning of silica surfaces in relation to their effectiveness for cleaning silica‐coated waveguide sensors used in OWLS. For this purpose, atomic force microscopy (AFM) analysis of surface morphology and OWLS detection of protein adsorption kinetics were used to evaluate waveguide sensors before and after cleaning. While AFM line scans showed a substantial increase in average waveguide peak‐to‐valley height after RCA cleaning relative to all other methods tested, chemical etching owing to the alkaline component of the rolling circle amplification method rendered the waveguide unusable for detection of protein adsorption with OWLS. A revised method, based on replacement of the alkaline step with immersion in sodium dodecyl sulfate, was not only effective at cleaning OWLS waveguides off‐the‐shelf but also showed excellent protein adsorption reproducibility after ex situ cleaning. Moreover, the revised method showed excellent reproducibility when applied in situ, between repeated adsorption‐elution cycles. Copyright © 2013 John Wiley & Sons, Ltd.     

基于核酸适配体的荧光分析法定量检测MUC1粘蛋白

MUC1粘蛋白是一种高糖化、高分子量的糖蛋白,其在乳腺癌细胞中高度异常表达,其特异性高于组织多肽抗原,敏感性高于癌胚抗原,因此MUC1在乳腺癌诊断中具有很高的临床应用价值,而建立高灵敏的MUC1蛋白定量检测方法对临床诊断具有重要的意义.该研究建立了基于核酸适配体-滚环扩增（RCA）和氧化石墨烯-荧光共振能量转移（GO-FRET）技术的MUC1黏蛋白定量检测技术,实现了MUC1粘蛋白准确、灵敏的定量检测.结果表明,该方法定量检测线性范围为50~1 000 pg/mL,检测限为28.05 pg/mL,定量限为45.57 pg/mL,在人血样品中的回收率为96%~104%.     

考虑再生粗骨料随机分布的混凝土氯离子扩散细观数值模拟

考虑粗骨料分布的随机性及氯离子结合的非线性对氯离子扩散性的影响,对再生混凝土中氯离子扩散进行数值建模与细观仿真分析,并将数值模拟结果与已有试验数据进行对比;通过改变老硬化砂浆扩散系数、新硬化砂浆扩散系数、老界面过渡区扩散系数及浸泡时间,对比研究了不同情况下再生混凝土中氯离子的扩散特性.结果表明:数值模拟结果与试验结果接近;再生混凝土中氯离子沿扩散深度方向呈波浪式下降;随着扩散系数的增大及浸泡时间的增长,氯离子扩散速度加快;随着再生粗骨料取代率的增加,氯离子扩散速度也增加;相同扩散深度处,不同随机骨料模型中氯离子含量最大值接近,说明所采用的随机骨料模型模拟结果具有较好的稳定性.     

浙鲁水产品出口竞争力比较分析

文章采用显示性比较优势指数从不同的角度对浙江、山东水产品的出口情况进行了比较分析,得出相关结论认为,虽然浙江与山东省水产品出口数量逐年增长,出口品种日趋多样化,但是浙江水产品出口尚处于外延型粗放发展阶段,如出口市场单一、出口商恶意竞争、水产品质量安全问题突出等。因此急需提高水产品质量。增加水产品附加值。     

A 5.3-GHz 32-bit accumulator designed for direct digital frequency synthesizer

A 32-bit pipeline accumulator with carry ripple topology is implemented for direct digital frequency synthesizer.To increase the throughout while hold down the area and power consumption,a method to reduce the number of the pre-skewing registers is proposed.The number is reduced to 29% of a conventional pipeline accumulator.The propagation delay versus bias current of the adder circuit with different size transistors is investigated.We analyze the delay by employing the open circuit time constant method.Compared to the simulation results,the maximum error is less than 8%.A method to optimum the design of the adder based on the propagation delay is discussed.The clock traces for the 32-bit adder are heavily loaded,as there are 40 registers being connected to them.Moreover,the differential clock traces,which are much longer than the critical length,should be treated as transmission lines.Thus a clock distribution method and a termination scheme are proposed to get high quality and low skew clock signals.A multiple-type termination scheme is proposed to match the transmission line impedance.The 32-bit accumulator was measured to work functionally at 5.3 GHz.     

两岸共同市场的贸易效应分析

通过两岸共同市场的构建现状分析，提出两岸共同市场在运行中存在的问题：台湾对大陆长期实施限制性的经贸政策，两岸经贸开放度不对称．运用RCA指数对两岸出口产品国际竞争力进行分析，得出大陆和台湾的优势产品类别不同，两岸进行的是互补性贸易，台湾当局不应该以经济规模不对称为借口对两岸经贸实施限制，两岸共同市场是海峡两岸经贸合作深化发展的最佳选择．     

我国服务贸易国际竞争力分析

运用国际市场占有率、贸易竞争力指数和显示性比较优势指数对我国服务贸易国际竞争力进行了分析。通过分析发现,我国服务贸易整体的国际竞争力还比较弱,其中资本技术密集型服务行业的竞争劣势尤为突出。     

两种再生粗骨料混凝土单轴受压性能研究

为研究影响再生混凝土力学性能的因素,将建筑结构中使用最为广泛的C30与C40两种强度等级的废混凝土破碎成再生粗骨料RCA(recycled coarse aggregate),根据实测RCA吸水率调整了配合比。以RCA来源和RCA取代率为变量,设计了9组再生混凝土试件,进行立方体抗压强度及棱柱体单轴受压试验。基于试验数据,得到了两种不同来源RCA的再生混凝土弹性模量、峰值应变等重要力学性能参数,绘制了再生混凝土应力-应变曲线。结果表明,随着RCA取代率的增加,两种不同来源RCA再生混凝土弹性模量和立方体抗压强度均表现为下降,RCA强度对再生混凝土各项力学性能均有影响。     

滚环DNA扩增的原理、应用和展望

滚环DNA扩增(Rolling circle DNA amplification,RCA)是一种等温信号扩增方法,其线性扩增倍数为10^5,指数化扩增能力大于10^9,产生的扩增产物连接在固相支持物(如玻片、微孔板等)表面的DNA引物或抗体上(适宜作生物芯片研究)。RCA是一种适合在芯片上(on-chip)进行信号扩增的新技术,它既能提供研究分析的敏感性和特异性,又能保持立体分析的多元性。RCA亦是一种痕量的分子检测方法,可用于极其微量的生物大分子和生物标志的检测与研究。,