现阶段高校就业指导队伍建设

讨论了高校大学生就业指导工作现状，对就业指导师资体系进行了研究，提出了现阶段就业指导队伍建设的基本框架。     

中美高校教师管理若干方面之比较

本文通过比较中美两国高校在教师职务 ,教师聘任 ,教师考核管理上的异同 ,分析其产成的原因及其对教育质量的影响 ,指出我国高校必须借鉴美国高校的管理经验 ,并结合我国的实际 ,建立有利于吸引人才、稳定人才、激励人才的教师管理制度。     

从系统论看服务主导型数字图书馆的人员结构

将系统理论引入数字图书馆研究中 ,介绍了数字图书馆的人员结构系统以及各个子系统的特点 .阐述了各个子系统和系统环境与用户服务之间的相互关系以及如何利用这种关系来提高数字图书馆的服务     

浅谈如何做好教学秘书工作

教学秘书队伍是一支重要的高等学校教学管理基层骨干力量。新形势下，做好教学秘书工作的关键在于不断提高从业人员的自身素质，认真做好每项日常工作，这样才能确保教学质量的提高。     

浅议保持共产党员先进性的必要性和时代要求

新时期保持共产党员先进性是由中国共产党的性质和宗旨决定的,是时代发展对我们党和党员提出的新要求,是新时期加强党的执政能力建设的基础工程。共产党员应具备与时俱进的精神状态,自觉落实执政为民。     

煤炭企业固定工的地位及现存问题的改进

本文对当前煤炭企业用工制度改革存在的问题、改进途径和各种形式用工的比例进行了分析,并指出为适应煤矿安全生产及机械化发展的需要,保持固定工的用工比例,克服“铁饭碗在手”等隋性意识,充分发挥他们的骨干作用是问题的关键。为此,本文提出了改变工资结构、劳动结构等相应的改革措施。     

二次受力下CFS加固混凝土构件的计算方法

碳纤维材料以其高强、轻质、耐腐蚀等优良性能广泛应用于混凝土结构加固领域。为了充分保证建筑结构的安全性，现以某办公楼加固工程为例，阐述了应用碳纤维材料对钢筋混凝土构件进行结构补强加固的设计原理，提出了考虑二次受力时应如何进行加固计算以及应用碳纤维布进行结构加固时应注意的事项等。     

开展经济效益审计是提升经济责任审计层次的必由之路

经济责任审计作为监督和制约领导干部正确行使权力的重要手段，直接关系到领导干部的选拔任用。多年来，审计工作一直停留在合法性、合理性层面上，实践证明具有一定的局限性。只有开展经济效益审计才能提升经济责任审计层次，充分发挥领导干部任期经济责任审计的职能和作用，更好地为我国社会主义经济建设服务。     

Yamogenin-Induced Cell Cycle Arrest,Oxidative Stress,and Apoptosis in Human Ovarian Cancer Cell Line

Steroidal saponins are a group of compounds with complex structures and biological activities. They have anti-inflammatory, antimicrobial, fungicidal, and antitumor properties. Yamogenin is one of the spirostane saponins and occurs in Trigonella foenum-graecum, Asparagus officinalis, and Dioscorea collettii. It is a stereoisomer of diosgenin—a well-known compound whose activity and mechanisms of action in cancer cells are determined. However, the antitumor effect of yamogenin is still little known, and the mechanism of action has not been determined. In this study, we evaluated the effect of yamogenin on human ovarian cancer SKOV-3 cells in vitro by determining the cellular factors that trigger cell death. The viability of the cells was assessed with a Real-Time xCELLigence system and the cell cycle arrest with flow cytometry. The activity of initiator and executioner caspases (-8, -9, and -3/7) was estimated with luminometry and flow cytometry, respectively. The mitochondrial membrane depolarization, the level of oxidative stress, and DNA damage in the yamogenin-treated cells were also evaluated by flow cytometry. Genes expression analysis at the mRNA level was conducted with Real-Time PCR. Bid activation and chromatin condensation were estimated with fluorescent microscopy. The obtained results indicate that yamogenin has cytotoxic activity in SKOV-3 cells with an IC₅₀ value of 23.90 ± 1.48 µg/mL and strongly inhibits the cell cycle in the sub-G1 phase. The compound also triggers cell death with a significant decrease in mitochondrial membrane potential, an increase in the level of oxidative stress (over two times higher in comparison to the control), and activation of caspase-8, -9, -3/7, as well as Bid. The results of genes expression indicate that the Tumor Necrosis Factor (TNF) Receptor Superfamily Members (TNF, TNFRSF10, TNFRSF10B, TNFRSF1B, and TNFRSF25), Fas Associated via Death Domain (FADD), and Death Effector Domain Containing 2 (DEDD2) were significantly upregulated and their relative expression was at least two times higher than in the control. Our work shows that yamogenin induces apoptosis in ovarian cancer cells, and both the extrinsic and mitochondrial—intrinsic pathways are involved in this process.     

既有大跨空间钢结构安全性评定方法

从构件角度对既有大跨度空间钢结构的安全性评定方法进行研究,根据<建筑结构可靠度设计统一标准>(GB 50068-2001)规定的结构构件承载能力极限状态的可靠度指标,提出既有钢结构构件的安全性等级应划分为安全与不安全两等级.基于荷载模型和抗力模型,确定了作用于既有大跨空间钢结构的各类荷载标准值.以分项系数确定的抗力与可靠度指标确定的抗力相对误差最小为原则编写可靠度程序,得到适合既有大跨空间钢结构评定的γG=1.2,γQ=1.3,以及各类构件的最优抗力分项系数.在综合考虑构件权重的基础上,提出了以整体性、承载安全性和整体稳定性的最低等级作为结构安全性等级的安全性评定方法.