Green and Straightforward Modification of Graphite Electrode via In Situ Synthesis of Graphene Nanosheets for Quantifying Prazosin Hydrochloride in Urine Samples and Pharmaceutical Formulations

A sensitive electrochemical sensor based on in situ modification of graphite electrode via graphene nanosheets (GNs) was developed as a green method for prazosin hydrochloride (PRA) analyses. In this study, GNs were electrochemically synthesized on the surface of graphite electrode via in situ approach and used for the analyses. The proposed sensor showed several advantages such as high sensitivity, low LOD, and excellent repeatability. The oxidation peak current at the optimum analytical conditions using a GNs/graphite electrode at pH 6.0 was linearly dependent on PRA concentration in the range of 0.09–100 µM. A LOD of 0.02 µM and RSD of 3.8 % for 10 µM solution of PRA with a great recovery were obtained.     

Simultaneous Determination of Prazosin,Atorvastatin, Rosuvastatin and Simvastatin in API,Dosage Formulations and Human Serum by RP‐HPLC

Prazosin hydrochloride is the first developed selective antagonist for α₁ — adrenoceptors, which is used as antihypertensive agent. Statins are used in the treatment of various types of hypercholesterolemia. In the present paper a simple, specific an accurate RP‐HPLC method was developed and validated for the simultaneous determination of prazosin, atorvastatin, rosuvastatin and simvastatin in active and in dosage formulations. A nucleosil 100‐10, C‐18, 10μ column having 250 × 4.6 mm i.d. in isocratic mode, with mobile phase containing methanol:water:acetonitrile (70:20:10) adjusted to pH 2.5 ± 0.02 using orthophosphoric acid. The flow rate was 1 mLmin^?1 and effluents were monitored at 240 nm. The % recovery for all the drugs in formulations was found to be 94‐105%. The parameters such as accuracy (%RSD less than 2), precision (%RSD less than 2), linearity (>0.999) were found to be satisfactory. The presented method was applied without any interference of excepients for the determination of tablets. The proposed method due to its low LOQ, excellent accuracy, precision and selectivity could be used for routine quality control.