Efficient separation of high‐purity compounds from Oxytropis falcata using two‐dimensional preparative chromatography

The separation of high‐purity compounds from traditional Tibetan medicines plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate compounds with high purity because of the complexity of traditional Tibetan medicines. In this work, an offline two‐dimensional reversed‐phase preparative method was successfully developed for the separation of high‐purity compounds from Oxytropis falcata . Based on the analysis results, an ODS C18 prep column was used for first‐dimensional preparation, and 14.8 g of the crude sample was separated into five fractions with a recovery of 74.6%. Then, an XAqua C18 prep column was used to isolate high‐purity compounds in the second‐dimensional preparation because its separation selectivity is different with the ODS C18 stationary phase. As a result, eight compounds in the crude sample were isolated in more than 98% purity. This is the first report of trans‐cinnamic acid ( 1 ) and trifolirhizin ( 2 ) from Oxytropis falcata . This method has the potential to be an efficient separation method of high‐purity compounds from Oxytropis falcata and it shows great promise for the separation of high‐purity compounds from complex samples.     

二色棘豆的染色体数目和核型分析

对二色棘豆（Oxytropis bicolor Bge.）的染色体进行了研究。结果显示染色体数目为2n=16,核型公式为K(2n)=2x=16=14m 2sm,相对长度组成为2n=16=8M2 8M2 8M1,核型为“1A”型。     

小花棘豆中黄酮甙的2D-NMR研究

利用2D-NMR并结合其它理化方法研究了从豆科植物小花棘豆中分得的一种黄酮甙:山奈酚-3-O-芸香糖甙的化学结构;该成分系首次从棘豆属植物中分得。     

砂珍棘豆之学名考

本文认为ＯｘｙｔｒｏｐｉｓｒａｃｅｍｏｓａＴｕｒｃｚ．与Ｏ．ｇｒａｃｉｌｌｉｍａＢｕｎｇｅ同为一种，砂珍棘豆的合法学名应为Ｏ．ｒａｃｅｍｏｓａＴｕｒｃｚ．     

Studies on the Chemical Constituents of Oxytropis Glabra DC (I)

Two new triterpenoid sapon.ins 1 and 2 were isolated from the crude saponin extract of Oxytropis glabra DC by Si gel chromato-grapny . Saponins 1 and 2 were determined to be 3-0-1 β-D-glucopyranosy 1 (1-2)- β -D-glucuronopyranosyl azukisapogenol methyl ester and 3-O-[β-D-glucopyranosyl(1 - 2 ) - β -D-glucuronopyranosyl] azukisapogenol amide, respectively by spectral analysis and chemical methods.     

黄花棘豆化学成分的研究（Ⅲ）

从黄花棘豆总皂苷的酸水解产物中分离出两种三萜皂苷元Ⅰ和Ⅱ,总皂苷经反复柱层析又得一微量三萜皂苷Ⅲ.本文报道它们的结构鉴定以及Ⅰ和Ⅱ的有关化学转变,用~(13)C NMR技术指定了Ⅰ和Ⅱ的~(13)C NMR信号.     

Chemical and Biological Characteristics of Oxytropis pseudoglandulosa Plant of Mongolian Origin

Oxytropis pseudoglandulosa is used in Mongolian traditional medicine due to its numerous reported health-promoting effects. To date, there are very few scientific reports that describe this species. In this article, its volatile oil composition, lipid extract composition, total phenolic and flavonoid content, antibacterial and allergenic properties are elucidated for the first time. Hexadecanoic acid, fokienol and tricosane were determined as the most notable components of the volatile oil, at 13.13, 11.46 and 5.55%, respectively. Methyl benzoate was shown to be the most abundant component of lipid extract at 40.69, followed by (E)-prop-2-enoic acid, 3-phenyl- and benzenepropanoic acid, at 18.55 and 9.97%. With a TPC of 6.620 mg GAE g⁻¹ and TFC of 10.316 mg QE g⁻¹, the plant extract of O. pseudoglandulosa indicated good antioxidant activity measured by IC₅₀ at 18.761 µg mL⁻¹. Of the 12 tested microorganisms, B. subtilis and S. cerevisiae were the shown to be most susceptible to the plant extract, with MIC at 2.081 and 0.260% (v/v), respectively. Bet v 1—a major birch pollen allergen found in plant-based foods—was determined to be at 192.02 ng g⁻¹ with ELISA. Such a wide spectrum of biological activity indicated by O. pseudoglandulosa lends credence for its application in food industry. Its exerted antioxidant and antimicrobial effects could improve preservation of low-processed food dedicated for consumers afflicted with allergies. Hexadecanoic acid supplemented in foods with dietary plant extracts could add to the potential anti-inflammatory impact. The analysis of lipid makeup suggests O. pseudoglandulosa extract could also be considered as natural pesticide in organic farming.     

大花棘豆在中国分布的研究

报道了大花棘豆在河北省的新分布,并通过对标本、资料的研究,对De Candolle所建立的大花棘豆的三个种下分类单位进行了修改.认为大花棘豆应包括二个变种分布于西伯利亚与我国河北省的原变种大花棘豆var.grandiflora和分布于内蒙古自治区及东北地区的变种狭翼大花棘豆var.leptoptera DC.     

关于Oxytropis oxyphylla （Pall.）DC.在中国的分布

本文认为ＯｘｙｔｒｏｐｉｓｈａｉｌａｒｅｎｓｉｓＫｉｔａｇ。与Ｏ．ｏｘｙｐｈｙｌｌα（ｐａｌｌ．）ＤＣ，同为一种，其合法有效学名应为后者。中国内蒙古呼伦贝尔和锡林郭勒典型草原区有其广泛分布。     

不同提取方法对小花棘豆总生物碱提取率的影响

采用乙醇浸提-有机溶剂萃取法、超声波辅助提取法、乙醇浸提-柱层析分离法3种方法提取小花棘豆中的总生物碱,比较3种方法总生物碱的提取率.3种方法的总生物碱提取率分别为0.0008％、0.0032％、0.0080％.故选用乙醇浸提-柱层析分离法.通过正交试验,得到乙醇浸提最佳工艺条件为:提取温度30℃,乙醇浓度75％,物料...