Metallothionein as a biomarker for mercury in tissues of rat fed orally with cinnabar

Cinnabar, as one of the most widely used mineral drugs in traditional Chinese medicines, has been proven to have prominent curative effects in clinical use for more than 2000 years. But the safety and toxicity of the drug has been under constant debate in clinic usage. Metallothionein (MT) contains about 30% of cysteine in the molecule, and plays an important detoxification role against heavy metals. In this study, it was used as a biomarker to assess mercurial accumulation in rats fed orally with cinnabar. After feeding rats with cinnabar by gastric gavage at different dosages and at different times, the distribution of heavy metals (including mercury, copper and zinc) and MT was investigated among rat tissues, including liver, kidney, heart, brain, testis and blood. Metals and MT determinations were carried out using inductively coupled plasma mass spectrometry (ICP‐MS) and a modified mercury saturation assay technique respectively. The results indicated that mercury was easily accumulated in the tissues of rats exposed to cinnabar, especially in kidney. For example: at a feeding dosage of 5 g kg^?1 (bw) for 4 weeks, the mercury concentrations in kidney were 13, 8.7, 21.6 and 26 times those in liver, testis, brain and heart respectively; and at 2.5 g kg^?1 (bw) for 2 weeks, the mercury concentrations in kidney were 21, 2.1, 3 and 21 times those in liver, testis, brain and heart respectively. In addition, mercury in kidney and liver of all cinnabar groups was significantly higher than that of the control group (P < 0.01). A high positive correlation observed between MT concentrations and mercury levels in both liver and kidney (R² = 0.9299, P < 0.02 for liver; R² = 0.9923, P < 0.0008 for kidney) indicated that MT could be used as a biomarker for mercury in tissues. Copyright © 2004 John Wiley & Sons, Ltd.     

井冈山珍稀濒危药用植物区系基本特征

介绍了井冈山自然保护区珍稀濒危药用植物的生态环境、种类以及地理分布区类型，并对其基本特征进行了分析，其中热带分布区的类型占优势，中国特有分布区类型突出，东亚和东亚至北美洲间断分布区类型明显。     

三清山旅游风景区蕨类植物区系研究

从三清山蕨类植物区系科属种统计，地理分布统计和江南山地蕨类植物区系比较分析，结果表明：该区系处于热带成分北迁，温带成分南渗及我国西南成分向华东、日本扩展的过渡地带，具有明显的东亚性、过渡性、复杂性和发生上的古老性等特征。     

井冈山第三次反“会剿”几个问题的考证

就湘赣敌军对井冈山革命根据地第三次“会剿”指挥人员的称谓和参加柏露会议的人员、柏露会议的内容以及如何看待彭德怀率守山军民突围下山等说法不一、值得探讨的问题，用历史原件和当事人回忆进行了考证。     

山西霍山森林群落主要种生态位的研究

将山西霍山每50m海拔段作为一个样方调查区,作四个100m~2的样方。从山蔍海拔900m到山顶2354m,共作样方52个。将海拔作为一条反映水、热、土壤等环境因子的综合资源轴,将群落类型作为反映种群互惠、竞争关系的另一综合资源轴。选择该区12种乔木,15种灌木,计测其在两条轴上的资源选择性系数(L)生态位宽度(B),和乔木之间,灌木之间以及乔灌木之间的生态重叠值(O)。结果表明L值愈小,B值愈大,物种的资源利用能力愈强,生态幅度愈大,对某一资源的依赖性愈小,反之亦然。比较同一资源轴上的不同的L值和B值,可反映物种的优势程度和区分能力。相同生活型种间的重叠值既可作为反映种间生态学相似性的指标。也可作为判断种间有无竞争可能的参考。不同生活型种间重叠值的的大小则可作为判断种间依存和相似程度的指标。     

Optimization of the irradiation power in chemical exchange dependent saturation transfer experiments

In chemical exchange dependent saturation transfer imaging experiments, exchangeable solute protons are saturated and the transfer of saturation to water is subsequently detected. When the applied irradiation power is comparable to the resonance frequency difference between the water protons and saturated solute protons, the proton transfer (PT) efficiency is reduced due to concomitant direct saturation effects. In this study, the PT process is modeled using a two-pool system. An empirical general proton transfer ratio (PTR) equation for arbitrary RF irradiation power is derived, and its optimal power to maximize the PTR is analyzed. The results are confirmed experimentally on 4.7 T using a poly-L-lysine solution. The theory provides a useful tool for optimizing the irradiation power of the PT sequences in the presence of direct saturation effects.     

浅论安源路矿工人运动对井冈山革命斗争的贡献

中国共产党在上世纪二十年代发动和领导的安源路矿工人运动,极大地提高了安源工人的思想觉悟,为此后的井冈山革命斗争打下了坚实的思想政治基础和革命骨干力量储备。经过安源路矿工人运动洗礼的工人及农民,成为井冈山革命斗争源源不竭的力量源泉。     

MT8981数字交换芯片的编程与应用

论文从数字交换芯片ＭＴ８９８１的内部硬件结构出发，介绍了怎样编程实现该芯片的数字交换功能、以及实现程控数字交换呼叫处理过程中，需由数字交换来协助完成的其它功能、其编程思想可拓广到ＭＩＴＥＩ公司同系列产品的其它数字交换芯片，广泛应用于诸如便携式用户小交换机、实验教学交换机以及语音信箱等通信产品的开发。     

Advantages of using a modified orthogonal sampling configuration originally designed for LC-ESI-MS to couple CE and MS for the determination of antioxidant phenolic compounds found in virgin olive oil

A ThermoFinnigan sheath liquid flow capillary electrophoresis-mass spectrometry system designed for coupling via a co-axial interface was coupled through an adapted via an alternative, commercially available interface for orthogonal sampling. The affordable, reversible structural alterations made in the commercial LC-MS interface resulted in improved analytical performance.The results of a conventional capillary electrophoresis (CE) method using a commercial co-axial source to determine antioxidant phenolic acids present in virgin olive oil, were compared with those obtained by using a modified orthogonal sampling position. In both cases, separations were done using a 10 mM ammonium acetate/ammonium hydroxide buffer solution at pH 10.0 and a constant applied voltage of 25 kV. The operating variables for the mass spectrometry interface were re-optimized for the modified orthogonal orientation. This allowed the sheath liquid, sheath gas flow rates and capillary voltage to be lowered with respect to the co-axial coupling configuration. In addition, the orthogonal sampling position provided a higher selectivity by effect of ion sampling excluding larger droplets—with an increased momentum along the axis—which were drained through the sink at the bottom of the ion source. Also, the new configuration facilitated sample ionization, improved electrospray stability and led to stronger signals as a result.The new system was validated in terms of precision (repeatability), linearity, and limits of detection and quantification. A comparison of the validation data with the results previously obtained by using a commercial co-axial configuration revealed the adapted orthogonal sampling position to provide better repeatability in both migration times and relative peak areas (<1% and 7% respectively with n = 15 replicates), a good linear range (with levels in the microgram-per-litre region) and lower limits of detection—especially for the compounds detected with the lowest sensitivity when co-axial ESI was used, as HFA, GEN, FER and VAN finding LOD among 24-3.0 μg L⁻¹ respectively.     

MT8880的应用及控制方法的实现

介绍了双音频接收送器MT8880在住宅智能化控制系统中的应用，给出了其与AT89C51的接口电路，同时，给出了模拟MT8880控制时序的软件设计。