排序方式: 共有22条查询结果,搜索用时 31 毫秒
1.
目的 基于小鼠尸体4 ℃冷藏不同时长后精子的体外受精(IVF)效率,探讨低温冷藏小鼠尸体应用于小鼠运输及意外死亡后品系恢复的可行性。方法 实验组为5月龄C57BL/6 J雄鼠,脱颈处死后4 ℃冷藏24、48、72 h和96 h,取附睾尾精子IVF,对照组为正常5月龄C57BL/6 J雄鼠IVF,统计各组IVF受精率。结果 冷藏24 h后附睾尾精子具备正常受精能力,受精率为73.0%、60.0%和77.0%;48 h 组内差异大,受精率为0%、55.4%和3.2%;72 h受精率极低,受精率为3.2%、0%和1.7%;96 h 精子有活力,但无受精能力。所有受精卵均可发育至2细胞,冷藏24 h组和对照组分别移植受体妊娠率分别为60%和66%,证明4 ℃冷藏对胚胎发育无影响。结论 C57BL/6J鼠经4 ℃冷藏的附睾尾,在24 h内具备稳定的受精率,可用于小鼠运输及特殊情况品系恢复。 相似文献
2.
牛体外受精胚胎移植的应用研究 总被引:4,自引:0,他引:4
探讨了利用牛IVF技术,在国外生产纯种肉牛胚胎运用国内进行大批量移植后受体母牛的妊娠率及产犊情况,共移植受体母牛158头,妊娠率(90d)为21.4% ̄48.5%,共计产纯种犊牛55头,5 ̄8日龄胚移植给发情第7d的母牛能够得到较高的妊娠率(43.8% ̄50.0%),受体母牛在前两交伯移植妊娠率(30.0% ̄35.0%),明显高于第三次(15.4%)以后的妊娠高,研究结果证实,在国外生产的的占IV 相似文献
3.
卵巢过度刺激综合征的防治 总被引:1,自引:1,他引:1
本院生殖医学中心13例卵巢过度刺激综合征(OHSS)的所有病例均发生在体外受精-胚胎移植及其衍生技术的诱导超排卵后。临床表现主要为腹胀、腹水、少尿、血液浓缩、低蛋白血症、水电解质失衡和氮质血症等,OHSS往往与高妊娠率伴随,轻、中度OHSS经系统监护,多不需特殊治疗;较重的患经人体白蛋白静脉输注,低分子右旋糖酐扩容,维持重要器官灌注等处理后治愈。 相似文献
4.
牛体外受精胚胎在成份确定培养液内的发育 总被引:1,自引:1,他引:1
将牛体外受精胚胎培养于化学成份确定的 SOF+PVA的培养系统内 ,观察了不同PVA浓度对胚胎体外发育的影响 .结果在 1 mg/ml、3mg/ml和 5mg/ml的 PVA浓度条件下 ,囊胚的发育率分别为 2 7.5% a、1 9.1 % a和 4 .3% b( a>b,P<0 .0 1 ) .将体外受精胚胎分别培养于 M1 99+输卵管上皮细胞、SOF+BSA和 SOF+PVA的三种培养系统内 ,囊胚的发育率分别为 2 0 .4 %、2 9.0 %和 2 7.5% ,结果表明本研究确立的化学成份确定的体外培养系统可以用于牛体外受精胚胎的发育培养 . 相似文献
5.
Accuracy of a combined score of zygote and embryo morphology for selecting the best embryos for IVF
下载免费PDF全文
![点击此处可从《浙江大学学报(自然科学英文版)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Objective: To evaluate the accuracy of a scoring system combining zygote and embryo morphology in predicting the outcome of in vitro fertilization (IVF) treatment. Methods: In a study group, 117 consecutive IVF or intracytoplasmic sperm injection (ICSI) cycles with embryo transfer were carried out and 312 embryos were scored using a combined scoring system (CSS) of zygote and embryo morphology before transplantation. In a control group, a total of 420 IVF or ICSI cycles were carried out and 1176 embryos were scored using a cumulative embryo score (CES). The effects of the combined scoring system on the embryo implantation rate and pregnancy rate per cycle were analyzed. Results: Using the combined scoring system, the embryo implantation rate (27.6%) and the clinical pregnancy rate (48.7%) were significantly higher than those in the control group (20.8% and 38.6%, respectively). Also, the implantation rate of embryos scoring ≥70 (38.5%: 82 sacs/213 embryos) was significantly higher (P〈0.001) than that of embryos scoring 〈70 (4%: 4 sacs/99 embryos). The pregnancy rate of patients with embryos scoring ≥70 using the combined scoring system (66.7%) was significantly higher (P〈0.001) than that of patients with embryos scoring ≥20 using the cumulative embryo score (59.0%). Conclusion: The results suggest that selecting embryos with a high score (≥70) using the combined scoring system could increase the implantation rate and pregnancy rate, and that using a scoring system combining assessments of human zygotes and pre-implantation embryos might predict IVF outcomes more accurately than using a cumulative embryo score. 相似文献
6.
7.
Masakuni SUZUKI 《Proceedings of the Japan Academy. Series B, Physical and biological sciences》2014,90(5):184-201
Assisted reproductive technology (ART) such as in vitro fertilization (IVF) and embryo transfer (ET) has been essential in the treatment of infertility. The world’s first IVF-ET baby was born in 1978 based on the technique developed by Dr. Robert Edwards and Dr. Patrick Steptoe.1) In Japan, the first IVF-ET birth was reported in 1983 by Prof. Masakuni Suzuki at Tohoku University School of Medicine.2,3)IVF-ET is a procedure used to achieve pregnancy that consists of extracting oocytes from an infertile woman, fertilizing them in vitro, and transferring fertilized eggs into the patient’s uterine cavity (Fig. (Fig.1).1). Since the first report of successful IVF-ET, numerous techniques related to ART, such as cryopreservation of oocytes and embryos, gamete intrafallopian transfer (GIFT), and microinsemination, have been developed and refined (Table (Table11).Open in a separate windowFigure 1. In vitro fertilization and embryo transfer process. A: Induction of ovulation: After menstruation, ovulation is induced to produce multiple oocytes. B: Testing: 1. Follicular diameter is measured using ultrasound. 2. Blood estradiol (E2) levels are measured. 3. Oocyte maturity is assessed with urinary Luteinizing Hormone (LH) measurements. C: Oocyte retrieval: Ultrasound-guided transvaginal follicle puncture is used to collect oocytes. D: Semen collection: Semen is collected from the husband. E: Sperm preparation: Semen is separated into multiple layers on a thickener effluent, and separated using a centrifuge. (400 × g, 30 min). F: Insemination: The semen and oocytes are placed together in a dish with culture media. G: Confirmation of fertilization: Fertilization is confirmed on the first day after oocyte retrieval. H: Embryo transfer: On the third or fifth day after oocyte retrieval, fertilized eggs are transferred to the uterus through a catheter.
Open in a separate windowHerein we describe the history of basic research in IVF-ET that led to human applications, how the birth of the first IVF-ET baby was achieved in Japan, the current status of ART in Japan, issues related to ART, and future prospects for ART. 相似文献
Table 1.
Timeline of reproductive technologies in Japan1983 | First baby conceived through in vitro fertilization was born | Tohoku University (Sendai, Miyagi Prefecture) |
1985 | First baby born using gamete intrafallopian transfer | Koshigaya Municipal Hospital (Koshigaya, Saitama Prefecture) |
1989 | First baby born from embryo cryopreservation and transfer | Tokyo Dental College Ichikawa General Hospital (Tokyo) |
1992 | First baby born from microinsemination (zona opening method) | Suzuki Memorial Hospital (Iwanuma, Miyagi Prefecture) |
1994 | First baby born from microinsemination (intracytoplasmic sperm injection) | Fukushima Medical University (Fukushima, Fukushima Prefecture) |
2001 | First baby born from in vitro fertilization after cryopreservation of mature oocytes | Suzuki Memorial Hospital (Iwanuma, Miyagi Prefecture) |
8.
研究探讨了不同体外培养条件下牛体外受精胚胎的发育速度及囊胚细胞数,从而了解不同体外培养系统对牛体外受精胚胎质量的影响.实验一中将体外受精卵分别在SOF+牛输卵管上皮细胞(BOEC)和TCM199+BOEC两种培养系统内培养,在这两种培养条件下囊胚发育率分别为22.2%和21.2%,囊胚细胞数分别为113.3±5.0和97.9±8.3,受精后第6~9d的囊胚出现率分别为40.1%a、37.0%、19.2%b、2.7%和13.5%c、32.7%、36.5%d、17.3%(a>c,P<0.01,d>b,P<0.05),表明牛体外受精卵在SOF+BOEC中的发育速度快于在TCM199+BOEC中.实验二中将体外受精卵分别培养于SOF+BOEC、SOF+牛卵丘细胞、SOF+BSA三种培养系统中,结果囊胚发育率分别为35.5%e、29.4%和22.4%f(e>f,P<0.01),囊胚细胞数分别为117.3±8.0g、94.2±9.3和90.2±9.4h(g>h,P<0.05).实验三观察了体外受精胚胎在SOF+BOEC培养条件下发育速度与囊胚细胞数的关系,结果第6~9d的囊胚细胞数分别为110.8±10.2i、128. 相似文献
9.
牛体外受精胚胎成份明确培养系统的建立 总被引:3,自引:0,他引:3
以 SOF为基本培养液 ,分别添加血清、PVA以及同时添加 PVA、肌醇和柠檬酸钠 ,进行牛体外受精卵的发育培养 ,三个处理组的囊胚发育率分别为 :3 2 .6%、1 1 .9%和 1 5 .9% ,血清处理组极显著高于两个 PVA处理组 (p<0 .0 1 ) .在此基础上 ,将受精处理后的卵子去掉卵丘细胞后 ,培养于 SOF PVA 肌醇 柠檬酸钠培养液内 ,以未去掉卵丘细胞的卵子为对照 ,两个处理组的囊胚发育率分别为 1 0 .8%和 2 1 .1 % .二者间没有显著差异 .结果表明没有血清和卵丘细胞的成份明确的培养系统能够支持牛体外受精卵发育至囊胚阶段 . 相似文献
10.
对哺乳动物体外受精(IVF)过程中多精受精的发生原因进行了阐述,指出多精受精的发生是影响各种哺乳动物体外受精效率的重要原因,其中在猪和人的IVF中表现得尤为突出。大量研究表明,除了物种的特异性以外,不完全的卵母细胞胞质成熟、异常透明带、高精子浓度、受精培养基中不适当的添加物以及在受精过程中的其他一些异常因素都与多精受精密切相关。 相似文献