Distribution of ghrelin-like immunoreactive cells in amphioxus, Branchiostoma belcheri – A study of immunohistochemistry

The distribution of ghrelin-like immunoreactive cells in amphioxus （Branchiostoma belcheri） was investigated by using immunohistochemical staining with rabbit antiserum against synthetical mammalian ghrelin. The results showed that ghrelin-like immunoreactive cells were distributed widely in the nervous system, Hatschek＇s pit, wheel organ, digestive tract and gonads （ovary and testis）. In nervous system, ghrelin-like immunoreactive neurons and their protrusions were distributed specifically on the dorsal side, ventral side and funnel part of brain vesicle, with a few dispersive immunoreactive nerve cells and their fibers in nerve tube. Ghrelin-like immunoreactivities were also detected in Hatschek＇s pit epithelial cells and wheel organ cells, with positive substance located along cell membrane. In digestive tract, ghrelin-like immunoreactive cells existed in hepatic diverticulum, anterior and posterior region of midgut, and could be classified into two types, closed- and opened-type endocrine cells. The number of positive cells was most in hepatic diverticulum, secondary in posterior region of midgut and least in anterior region of midgut. In gonads, ghrelin-like immunoreactive substance was detected in oogonia, oocytes and follicle cells in ovary at the small and large growth stages and in early spermatogenic cells and Sertoli cells in testis. The extensive distribution of ghrelin-like cells in amphioxus suggested that these kinds of cells are conservative in evolution and diversified in function. At the same time, we found for the first time that ghrelin-like immunoreactive cells existed in brain vesicle and Hatschek＇s pit, which provided new morphological evidence for the existence of an activation pathway between brain vesicle and Hatschek＇s pit for the regulation of growth hormone excretion.     

拟黑多刺蚁(Polyrhachis vicina)性腺雌激素相关受体基因的表达

采用原位杂交方法对拟黑多刺蚁(Polyrhachis vicina)蚁后卵巢中和雄蚁精巢中pvERR mRNA的表达进行了检测.结果表明,pvERR mRNA在卵子发生过程中滤泡细胞的胞质和生长期、卵黄发生期的卵母细胞有表达.在变形期的精细胞胞质和成熟精子头部有很强的表达.     

家鸭属间杂种及其亲本生殖腺组织化学的比较研究

对性成熟前后的金定鸭，正番鸭及其属间杂种正交系Ｆ１性腺中糖元，ＡＣＰ，ＡＫＰ，ＬＤＨ及其同工酶以及３β－ＨＳＤ和１７β－ＨＳＤ作了组织化学定位。与亲本相比，１１０日龄前后的杂 种Ｆ１曲精细管已处于异常分裂状态，２１０日龄左右的Ｆ１曲精细管内ＡＣＰ活性极强，其精子形成过程在精细胞早期中止。杂种Ｆ１精细胞无法变态可能与支持细胞功能异常以及曲精细管内缺乏适合精子形成的微环境有关。     

繁殖季节黄颡鱼[Pseudobugrus fucridraco(Rich)]性腺的研究

记叙了5月份野生黄颡鱼性腺的基本情况,描述了黄颡鱼雄性生殖细胞和雌性生殖细胞的形态特征及发生规律.     

口虾蛄Oratosquilla oratoria （De Huan）性腺特征及卵巢组织学观察

本文描述了口虾蛄精巢和卵巢的基本特征，并对卵巢进行了组织切片显微观察。结果认为，口虾蛄精巢由１对很细的管状结构组成，精子圆球形，无鞭毛。卵巢左右两叶，对称且相互粘连。卵母细胞发育分为生长期、卵黄形成前期、卵黄形成期和成熟期四期。卵巢发育分为未发育期、发育期、成熟前期、成熟期和产卵期五期。在发育期和成熟前期的卵巢中，左右两叶各有一个“Ｓ”形的“增殖中心”。