Development and validation of a rapid and sensitive assay for the determination of anisodamine in 50 μL of beagle dog plasma by LC–MS/MS

A simple, rapid, high‐throughput, and highly sensitive LC–MS/MS was developed to determine anisodamine in a small volume (50 μL) of beagle dog plasma using atropine sulfate as the internal standard. The analyte and internal standard were isolated from 50 μL plasma samples after a one‐step protein precipitation using Sirocco 96‐well protein precipitation filtration plates. The separation was accomplished on a Hanbon Hedera CN column (100 × 4.6 mm, 5 μm) and the run time was 4 min. A Micromass Quatro Ultima mass spectrometer equipped with an ESI source was operated in the multiple reaction monitoring mode with the precursor‐to‐product ion transitions m/z 306.0→140.0 (anisodamine) and 290.0→123.9 (atropine) used for quantitation. The method was sensitive with a low LOQ of 0.05 ng/mL, and good linearity in the range 0.05–50 ng/mL for anisodamine (r² ≥ 0.995). All the validation data, such as accuracy, intra‐ and interrun precision, were within the required limits. The method was successfully applied to the pharmacokinetic study of anisodamine hydrochloride injection in beagle dogs.     

Beagle犬体重与各脏器重量之间关系的回归分析

对４５只Ｂｅａｇｌｅ犬的体重与各脏器重量之间的关系进行了回归分析，并计算出相应的直线回归方程。对Ｂｅａｇｌｅ犬的脏器系数进行分析，显示脾脏、胸腺的变异系数较大，提示根据动物的脾脏、胸腺重量来评价其免疫功能时，要慎重考虑。     

不同月龄Beagle犬的心电图研究

对不同月龄的６６只健康Ｂｅａｇｌｅ犬采用３％戊巴比妥钠麻醉，进行了９个导联的心电图描记和分析。结果表明：健康Ｂｅａｇｌｅ犬为窦性心律：３０月龄以下育成犬心律平均为１７３．８次／分，种用犬（５０月龄以上）心率平均为１３１．７次／分；二者经统计学处理，有极显著差异。Ｐ波间期平均为０．０５１±０．０１３Ｓｅｃ。Ｐ波振幅平均０．０６１ｍｖ，Ｐ波直立在导联中占９２．６％，在ａＶＲ导联中Ｐ波倒置率９８．７５％；ＱＲＳ波间期均值为：０．０２７±０．００５Ｓｅｃ。在Ⅱ导联中范围为０．０２－０．０４Ｓ－ｅｃ。在各导联中，Ⅰ导联以ｒ波为主（５１．８５％），Ⅱ导联以Ｒｓ波多见（５０％），Ｑｒ波和ｑｒ波在ａＶＲ导联中出现较多（３８．８９％，３１．４８％）。Ｔ波在Ⅰ、Ⅱ、Ⅲ、ａｖＦ、Ⅴ１、Ⅴ２、Ⅴ３导联中直立为主，在ａｖＬ导联中倒置多见（８９．２％，７３．１％）。Ｔ波方向与ＱＲＳ波一致。Ｔ波振幅平沟０．１３７ｍｖ。Ｐ－Ｒ间期范围在此０。０７５－０．１４０Ｓｅｃ。在９个导联中，无明显Ｓ－Ｐ段偏移。Ｐ－Ｐ间隔＜０．０１Ｓｅｃ。     

导盲犬和淘汰犬肠道菌群的差异性研究

目的 探究导盲犬和淘汰犬之间肠道菌群的差异,分析两者特征性差异菌群。方法 收集16只导盲犬和10只淘汰犬的新鲜粪便样本,按照犬品种和性别分为拉布拉多雄性导盲犬(GD-ML)和淘汰犬(ED-ML)、拉布拉多雌性导盲犬(GD-FL)和淘汰犬(ED-FL)、金毛雄性导盲犬(GD-MG)和淘汰犬(ED-MG),另外从中随机抽取9只导盲犬和4只淘汰犬分为导盲犬组(GD)和淘汰犬组(ED),共分为4组配对。提取粪便样本总DNA,应用PCR-DGGE技术获得肠道菌群图谱,应用相关软件和统计学方法对每组配对进行差异性分析。结果 聚类分析结果显示,GD和ED,GD-ML和ED-ML,GD-FL、ED-FL和GD-MG均各归为一类,表明导盲犬和淘汰犬之间肠道菌群存在差异。肠道菌群多样性、丰富度和均匀度分析结果显示,每两个配对组之间多样性指数、丰富度指数和均匀度指数存在差异,但差异均无统计学意义。差异性条带测序分析结果显示：与ED相比,GD Megamonas funiformis YIT 11815菌株增多;与ED-ML相比,GD-ML Succinatimonas hippei YIT 12066、Lactobacillus vaginalis DSM 5837、Lactobacillus acidophilus NCFM、Faecalibacterium prausnitzii A2-165菌株增多,Collinsella aerofaciens ATCC 25986和Prevotella copri DSM 18205菌株减少;与ED-FL相比,GD-FL Ruminococcus gnavus AGR2154、Fusobacterium russii ATCC 25533菌株减少;与ED-MG相比,GD-MG Tropheryma whipplei str. Twist菌株增多。结论 本研究发现导盲犬和淘汰犬肠道菌群存在差异,未来对差异菌群的进一步研究可能有助于辅助导盲犬的早期筛选。     

陈与郊与其杂剧《袁氏义犬》

万历年间,戏曲家陈与郊创作的杂剧《袁氏义犬》,其题材虽来源于历史故事,却带有浓重的自传色彩。该杂剧具有两个重要的特点：一个是将王衡的杂剧《没奈何》插入第一出,采用了“戏中戏”的艺术手法,形成了别具一格的戏剧效果;另一个是陈与郊有心将该剧写成一部“寓言剧”,并通过一个寓言故事的演述来寄寓和抒写自己的内心情感。     

Dog Bone栅型MOS场效应晶体管的等效宽长比计算

Dog Bone栅型MOS场效应晶体管是一种对称性的、防辐射总剂量效应的版图结构。为了计算其等效宽长比,把它划分为常规结构的主MOS晶体管和非常规结构的边缘MOS晶体管2种类型的并联。借助Silvaco TCAD工艺与器件仿真工具构建了它的模型,分析了主MOS晶体管的宽度、长度和边缘MOS晶体管的多晶硅与有源区的交叠宽度对边缘MOS等效宽长比的影响,得到拟合的Dog Bone栅型MOS晶体管等效宽长比的计算公式。采用CSMC 0.5μm DPTMCMOS混合信号工艺制作了样管,对实验测量值和公式计算值进行比较,Dog Bone栅型MOS场效应晶体管的等效宽长比的计算公式与实验能够较好地吻合。     

Improved and Rapid Liquid Chromatographic Determination of Indomethacin in Serum

Abstract

A rapid, specific and sensitive reversed-phase liquid chromatographic (LC) assay for the quantitative determination of indomethacin in serum without extraction was developed. Chromatographic separation using flunixin meglumine as the internal standard was achieved on octadecylsilane-coated particles with a mobile phase of 0.15 M acetate buffer pH 3.0 (50% v/v), acetonitrile (30% v/v) and methanol (20% v/v). The recovery of indomethacin from serum samples in the concentration range of 0.1-25 μg/ml was 95.5 ± 5.8% and as little as 100 ng/ml of indomethacin in serum samples can be quantitated by this procedure. A serum level versus time profile of dog with intravenously administered indomethacin demonstrated the applicability of the assay.     

Fundamental frequency and tracheal pressure during three types of vocalizations elicited from anesthetized dogs

Electrical stimulation of the midbrain was used to elicit a variety of vocalizations from six anesthetized dogs. This study was conducted to investigate the ranges of and relationships between fundamental frequency of the vocalizations (F₀) and tracheal pressure (P_t) produced during the vocalizations. The vocalizations were described according to type (growl, howl, and whine); F₀ and P_t, as well as patterns of laryngeal muscle activity, were examined for each vocalization type. Natural-sounding growl and howl vocalizations were elicited from five dogs; three dogs also produced whines. With few exceptions, F₀ was categorically different for the three vocalization types (low for growls, average for howls, very high for whines). P_t values overlapped for the three vocalization types, although, on average, howls were produced with greater P_t than growls. Patterns and degrees of laryngeal muscle activity varied across and within vocalization types, but general findings were consistent with the presumed function of most of the muscles. Laryngeal muscle activity may help explain some of the variability in the acoustic and aerodynamic data.     

发情犬与未发情犬卵母细胞体外受精的研究

采集发情犬和未发情犬卵巢,回收大腔（直径大于２ｍｍ）、小腔（直径１－２ｍｍ）和不可见卵泡卵细胞,进行体外受精,结果如下：①发情组的单精受精率（３１．２％）,原核形成率（１９．４％）高于未发情组。发情组的多精受精率（１２．６％）要低于未发情组。②直径１－２ｍｍ、大于２ｍｍ卵泡组单精受精率略高于不可见卵泡组。可见卵泡组的原核形成率高于不可见卵泡组。③发情犬卵泡直径大于２ｍｍ组卵裂率高于发情犬和未发情犬     

两种麻醉剂在犬外科实验中麻醉效果的比较

目的　探讨戊巴比妥钠和苯巴比妥钠在犬外科实验中的麻醉效果。方法　实验犬 12 0只 ,随机分为两组 ,分别用 3%戊巴比妥钠注射液 (剂量 4 5mg/kg)和 5 %苯巴比妥钠注射液 (剂量 10 0mg/kg)行腹腔内注射麻醉 ,对比观察两组动物的各项麻醉指标。结果　戊巴比妥钠组的麻醉起效时间明显短于苯巴比妥钠组 (P 相似文献