A study for quality evaluation of Taxilli Herba from different hosts based on fingerprint-activity relationship modeling and multivariate statistical analysis

In this study, a fingerprint-activity relationship modeling between chemical fingerprints and antirheumatic activity was established, and multivariate statistical analysis was used to evaluate the quality of Taxilli Herba (TH) from different hosts. Characteristic fingerprints of 20 batches of TH samples were generated by high-performance liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (HPLC-Triple TOF-MS/MS), and the similarity analysis was calculated based on thirteen common characteristic peaks by hierarchical clustering analysis (HCA). Subsequently, nine efficacy markers were discovered by combining fingerprints and antirheumatic activity through grey correlation analysis (GCA) and bivariate correlation analysis (BCA). Meanwhile, the content of 5 constituents in 9 markers was determined by high-performance liquid chromatography coupled with triple quadrupole-linear ion trap tandem mass spectrometry (HPLC-QTRAP-MS/MS). The comprehensive quality of TH was assessed using multivariate statistical analysis, including principal components analysis (PCA) and technique for order preference by similarity to ideal solution (TOPSIS). The results showed that a high dose of TH extract could markedly ameliorate arthritis damage compared to other doses, with flavonoids playing an important role in the antirheumatic activity. The comprehensive quality of samples from Morus alba L. (SS) was superior to those from Liquidambar formosana Hance (FXS). The present study will demonstrate the markers associated with efficacy, and provide an applicable strategy for more comprehensive quality control and evaluation of TH.     

动物实验管理委员会的职责

介绍了美国、英国、法国、日本、德国、澳大利亚等国家有关实验动物的法律法规,介绍了各国各级动物实验管理委员会的组成、职责和管理.呼吁我国应尽快地健全法制化管理,加强基层动物实验管理委员会的建设和明确其职责.     

湖北省高校1971～1990年间教职工全死因回顾性整群抽样调查

通过对湖北省高校近20年教职工死因调查,报道了该省高校1971～1990年20年间共死亡教职工680人,总死亡率为329.462/10万的情况。前三位死因顺位依次为恶性肿瘤、脑血管病、心血管病。恶性肿瘤的前三位死因顺位是肝癌、肺癌、胃癌。死亡年龄分布40岁以上占死亡总数的90.15％。同时对不同职别、性别群体的死因进行了分析,对有关因素略作讨论,并提出高校应积极开展健康教育、建立与完善健康咨询、推行综合健康医学模式等教职工的最佳保健方案。     

Critical evaluation of the determination of pharmaceuticals, personal care products, phenolic endocrine disrupters and faecal steroids by GC/MS and PTV-GC/MS in environmental waters

An analytical method is described for the determination of a broad range of emerging and priority pollutants, together with sewage molecular markers in environmental waters. The step-by-step study of the GC/MS analyses focuses on the effects of experimental variables using a large volume injection (LVI) technique [a programmed temperature-vaporising (PTV) inlet], the evaluation of a clean-up step using classical and newer sorbents (i.e. Al-N, Fl, NH₂, PSA, Si, CN and DIOL), and the revision of how organic matter [i.e. humic acids (HA) content] affects method performance. Reproducibility and recoveries from spiked coastal water samples at different analyte concentrations (100, 250 and 500 ng L⁻¹) as well as with different levels of spiked humic acids (2, 10 and 20 mg L⁻¹) are reported indicating a good performance of the extraction procedure with low levels of HA (<10 mg L⁻¹). The presence of HA is a critical parameter during the solid-phase extraction (SPE) procedures. Of the clean-up sorbents tested, CN and DIOL proved most efficient in cleaning-up the extracts with recoveries in the range of 66-77% and 100-114%, respectively for the selected analytes. Both GC/MS and PTV-GC/MS instrumental configurations were tested using final sewage effluents, riverine, estuarine and coastal water samples. However, limited applicability of the PTV inlet is reported for environmental applications, affording only a modest improvement in chromatographic signal-to-noise ratios.     

Changes in Hip and Knee Muscle Strength in Patients Following Total Hip Arthroplasty

Objective: To investigate changes in hip and knee muscle strength in patients before and after total hip arthroplasty (THA) in comparison with that in healthy adults. Methods: The study included 21 women who underwent unilateral THA (THA group) and 21 age-matched healthy women (healthy group). Maximal isometric strengths of hip flexors, extensors, and abductors, and knee extensors and flexors were measured before surgery and at 4 weeks and 6 months after surgery. Results: Before surgery, muscle strength on both sides, except for hip flexors on the uninvolved side, was significantly lower in the THA group than the corresponding muscle strength in the healthy group. Up to 6 months after THA, strength of all muscle groups on both sides was significantly improved compared with their preoperative status, although the knee extensor strength on the involved side temporarily worsened at 4 weeks. However, the strength of hip extensors and knee extensors on the involved side, and hip abductors on both sides in the THA group remained below that in the healthy group. Conclusions: Our results suggest that rehabilitation specialists should consider increasing the focus on the uninvolved side and encourage patients to continue strength training beyond 6 months after surgery.     

Spectrophotometric determination of nitrite with p-nitroaniline and 2-methyl-8-quinolinol in hexadecyl-trimethylammonium bromide solution

Nitrite ion at low concentrations is determined spectrophotometrically by diazotization of p-nitroaniline and coupling of the diazonium salt with 2-methyl-8-quinolinol. The resulting dye is solubilized in hexadecyltrimethylammonium bromide micelles. The molar absorptivity is 4.72 × 10⁴ l mol^-1 cm^-1, and the Sandell sensitivity is 9.7 × 10^-4 μg cm^-2. Some interferences are reported, and preconcentration by evaporation is evaluated. The solubility of hexadecyltrimethylammonium bromide in water was determined as a function of temperature; the Krafft point is 19.6°C. Salting-in of the surfactant by potassium nitrate is described.     

《体育保健学》教学提问原则的探索

在<体育保健学>的教学中,教师向学生所做的高质量的提问,有助于提高教学质量,调动学生的学习积极性,较好地完成教学任务并实现教学目的.提问内容的确定、难易的把握及提问的系统性,应贯彻典型性和启发性相结合、适度与梯度相统一、回顾与前瞻相连接的原则.     

做好一名高职数学教师的一点体会

要做好一名高职数学教师,必须做到专心、用心、热心、信心、耐心及关心。专心提高自己的业务素质,用心结合学生的专业,热心帮助和关心学生,树立信心,坚持不懈,就一定能成为一名优秀的高职数学教师。     

略论高校管理理念的变革

现代社会对高校管理的认识不应仅仅停留在科学管理的层面上,还应体现人本管理的精神,并且将两者有机地结合起来。高等学校的教师和科研人员的成熟程度以及高等学校的职能活动,决定教师和科研人员是高校管理的决策主体,行政人员是高校管理的执行主体。但现在"行政权力主导"的管理理念在我国高校影响很深。现实要求用"学术权力和行政权力各司其职"的新管理理念来指导高校的改革,并通过改革来强化这种新的管理理念。