A study for quality evaluation of Taxilli Herba from different hosts based on fingerprint-activity relationship modeling and multivariate statistical analysis

In this study, a fingerprint-activity relationship modeling between chemical fingerprints and antirheumatic activity was established, and multivariate statistical analysis was used to evaluate the quality of Taxilli Herba (TH) from different hosts. Characteristic fingerprints of 20 batches of TH samples were generated by high-performance liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (HPLC-Triple TOF-MS/MS), and the similarity analysis was calculated based on thirteen common characteristic peaks by hierarchical clustering analysis (HCA). Subsequently, nine efficacy markers were discovered by combining fingerprints and antirheumatic activity through grey correlation analysis (GCA) and bivariate correlation analysis (BCA). Meanwhile, the content of 5 constituents in 9 markers was determined by high-performance liquid chromatography coupled with triple quadrupole-linear ion trap tandem mass spectrometry (HPLC-QTRAP-MS/MS). The comprehensive quality of TH was assessed using multivariate statistical analysis, including principal components analysis (PCA) and technique for order preference by similarity to ideal solution (TOPSIS). The results showed that a high dose of TH extract could markedly ameliorate arthritis damage compared to other doses, with flavonoids playing an important role in the antirheumatic activity. The comprehensive quality of samples from Morus alba L. (SS) was superior to those from Liquidambar formosana Hance (FXS). The present study will demonstrate the markers associated with efficacy, and provide an applicable strategy for more comprehensive quality control and evaluation of TH.     

接骨草染色体核型分析

以接骨草为实验材料,按常规制片方法,观察中期分裂相并进行显微摄影。染色体类型按Levan方法分类,其核型为k(2n)=34=18m 16 sm。根据Sebb ins的染色体核型的不对称类型,接骨草染色体属于“2B”型。     

微红梢斑螟的研究

微红梢斑螟(Dioryctria rubella Hampson),是松类梢果重要害虫,分布全国,幼虫钻蛀主梢及球果,在南京每年发生2～3代,以幼虫在枯梢及树皮伤口内越冬,次年5月转移为害当年新梢。成由于6至8月出现,生活史不整齐。本文报道各虫期生物学、生态学特性及防治试验结果,并讨论了防治策略问题。     

基于adina的土钉支护有限元数值模拟

建立土钉支护的非线性有限元模型,土体本构采用Mohr-Coulomb模型,土钉之间采用Goodman单元模拟,能够模拟基坑的开挖与支护的施工过程。对某一具体基坑工程进行了模拟计算,取得了较满意的结果。     

人工林青梅木纤维长度变化规律初探

对人工林青梅木材进行了离析.通过观测,得到如下结果：木纤维长度在树木幼期随离髓距离（生长轮数）的增加而增加,并随离地高度的增加先增加,达到最大后下降.全株纤维长度范围240μm～1 600μm,平均长773.3μm.     

Simultaneous identification and analysis of cassane diterpenoids in Caesalpinia minax Hance by high‐performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry

Cassane diterpenoids were successfully and simultaneously identified in Caesalpinia minax Hance by high‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry. A total of 59 peaks were detected, and among them 51 compounds, including 41 furanocassane diterpenoids, 10 furanolactone cassane diterpenoids were simultaneously identified and characterized on the basis of the protonated molecule, retention behavior, and fragments in MS². Ten compounds, including seven novel compounds, were identified or tentatively identified for the first time in C. minax. In a positive ion mode, the fragmentation pathways of cassane diterpenoids were also analyzed for the first time. The relative amounts of the five main diterpenoids (caesalpinin L, caesalpinin F₂, bondcellpin C, caesalpinin E, and ξ‐caesalmin) were simultaneously quantified by high‐performance liquid chromatography. Results showed that the newly discovered and known components of C. minax can be used to determine the material basis of bioactivity; this method can also be applied to analyze cassane diterpenoids in herbal medicines from the genus Caesalpinia belonging to the family Fabaceae.     

枫香根际解有机磷细菌筛选及其促生效应

从枫香（Liquidambar formosana Hance）根际土壤中分离和鉴定有效解磷菌（PSB），并研究其在溶磷方面的应用。     

Extraction and isolation of diphenylheptanes and flavonoids from Alpinia officinarum Hance using supercritical fluid extraction followed by supercritical fluid chromatography

In this paper, an off-line combination method of supercritical fluid extraction and supercritical fluid chromatography was developed for the selective extraction and isolation of diphenylheptanes and flavonoids from Alpinia officinarum Hance. The enrichment of target components was successfully achieved using supercritical fluid extraction with the following conditions (8% ethanol as co-solvent at 45°C and 30 MPa for 30 min). Taking full advantage of the complementarity of supercritical fluid chromatography stationary phases, a two-step preparative supercritical fluid chromatography strategy was constructed. The extract was firstly divided into seven fractions on a Diol column (250 × 20 mm internal diameter, 10 μm) within 8 min by gradient elution increasing from 5% to 20% modifier (methanol) at 55 ml/min and 15 MPa. Then the seven fractions were separated by using a 1-AA or a DEA column (250 × 19 mm internal diameter, 5 μm) at 50 ml/min and 13.5 MPa. This two-step strategy showed superior separation ability for structural analogs. As a result, seven compounds, including four diphenylheptanes and three flavonoids with high purity, were successfully obtained. The developed method is also helpful for the extraction and isolation of other structural analogs of traditional Chinese medicines.     

珊瑚姜化学成分及精油研究开发进展

珊瑚姜是少数民族的一种民间习用药物,具有很大的潜在药用价值,对2003年以来珊瑚姜化学成分及精油的研究与开发进展进行了综述,指出深入研究珊瑚姜的非挥发油化学组分,寻找其有用的活性物质,开辟新的药用价值是其今后的研究方向。     

制备色谱法分离纯化高良姜黄酮中高良姜素和山柰素

建立了制备型高效液相色谱(Prep-HPLC)分离高良姜黄酮中高良姜素和山柰素的方法。高良姜黄酮经HPD-600树脂吸附洗脱纯化后,采用Prep-HPLC分离高良姜黄酮中高良姜素和山柰素。制备色谱条件:流动相为甲醇-0.6%(v/v)乙酸水溶液(58:42, v/v),柱温为常温,流速为7.0 mL/min,检测波长为360 nm,进样量为700 μ L,上样质量浓度为10.0 g/L。分离的单体由质谱和核磁共振氢谱、碳谱鉴定确证为高良姜素和山柰素,HPLC外标法定量,纯度分别为99.5%和99.7%。该方法分离效果好、高效、低毒,可用于高良姜中高良姜素和山柰素的分离制备。