闽产土牛膝抑菌活性的研究

用传统煎煮中药的方法制备土牛膝药液,对其中能抑制诱发慢性扁桃体炎的需氧致病菌的有效成分进行分析.结果表明,能抑制诱发慢性扁桃体炎的需氧致病菌的有效成分主要为水溶性物质,并且其相对分子量大于10 0 0 0 .     

植物生长物质对牛膝生长及主要药用成分积累的影响

采用不同植物生长物质组合对牛膝幼苗进行喷施处理和植物化学分析相结合的方法,研究植物生长物质对牛膝生长及其主要药用成分合成的影响.结果表明:1.0mg·L-1 IBA+1.0mg·L-1 MJ(T4)协同处理最有利于牛膝地下部分生物量的增加;1.0mg·L-1 IBA+1.0mg·L-1 6-BA(T3)协同处理最有利于牛膝根中齐墩果酸的积累;1.0mg·L-1 IBA+2.0mg·L-1 NAA(T2)和1.0mg·L-1 IBA+1.0mg·L-1 6-BA(T3)处理最有利于牛膝根中蜕皮甾酮的积累.综合考虑各项指标,1.0mg·L-1 IBA+1.0mg·L-1 6-BA(T3)处理无论是对牛膝的生长还是主要药用成分三萜皂苷和蜕皮甾酮的积累均比对照有显著性提高,最有利于牛膝药材产量和品质的提高.     

牛膝中齐墩果酸和β-蜕皮甾酮的HPLC法含量测定及指纹图谱研究

建立了中药牛膝的HPLC指纹图谱并同时测定其中齐墩果酸和β-蜕皮甾酮的含量。采用Agilent ZORBAX SB C18色谱柱,流动相为乙腈 0.6%甲酸,柱温为25 ℃,检测波长为260 nm,进行了13批次牛膝药材的分析。牛膝药材的HPLC指纹图谱共有16个共有峰,4个特征峰,定量测定了其中两个峰β 蜕皮甾酮和齐墩果酸。通过相似度分析初步断定,不同地域的牛膝药材在质量上存在明显的差异。该研究为建立牛膝药材的质量控制体系提供了方法和技术支持。     

摘花序和打顶对怀牛膝叶绿素荧光特性和品质的影响

以现蕾期怀牛膝为材料,研究了摘除全部花序、打顶等处理对怀牛膝生育性状特征、叶绿素含量、叶绿素荧光特性、根部产量和多糖含量的影响.结果表明,现蕾期摘除全部花序后,怀牛膝植株生长健壮,叶绿素含量升高,Fw/Fo,Fv/Fm和ETR升高.潜在光合性能增强,根部产量和多糖含量升高;打顶后植株生殖生长旺盛,根部增产效果不明显.     

钾营养对牛膝生物量和多糖含量的影响

研究了钾元素对牛膝生物量和牛膝根中可溶性多糖含量的影响,设定了4个钾离子水平,测定了根和冠的生物量,根的增粗情况以及根中K^ 浓度和可溶性多糖含量。发现牛膝根可缓冲环境[K^ ]对其植株生长的影响。且对K^ 有很强的富积能力。K^ 可促使营养物质向牛膝根运输,促进牛膝根的增粗,提高单株牛膝根中可溶性多糖含量。     

Studies on Synthesis and Antitumor Activity of Phosphorylated Achyranthes bidentata Polysaccharide (P-AbPS)

ＩｎｔｒｏｄｕｃｔｉｏｎＩｔｉｓｗｅｌｌｋｎｏｗｎｔｈａｔｔｈｅｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅｓａｒｅｃａ ｐａｂｌｅｏｆｒｅｖｅａｌｉｎｇａｖａｒｉｅｔｙｏｆｂｉｏｌｏｇｉｃａｌａｃｔｉｖｉｔｉｅｓｉｎｃｌｕｄ ｉｎｇｔｕｍｏｒ ｇｒｏｗｔｈｉｎｈｉｂｉｔｏｒｙａｃｔｉｖｉｔｙ .1Ｐｈｏｓｐｈｏｒｙｌａｔｉｏｎｏｆｐｏｌｙｓａｃｃｈａｒｉｄｅｓｈａｓａｔｔｒａｃｔｅｄｗｉｄｅｓｐｒｅａｄａｔｔｅｎｔｉｏｎｂｅｃａｕｓｅｉｔｗａｓｒｅｐｏｒｔｅｄｔｈａｔｔｈｅｙｓｈｏｗａｎｔｉｔｕｍｏｒａｃｔ…     

羟乙基化牛膝多糖的合成及其活性研究

以环氧乙烷为羟乙基化试剂，在碱性水溶液中，对牛膝多糖进行羟乙基化，经 过丙酮沉淀、膜分离、Sephadex G-25柱层析等分离方法得到羟乙基化牛膝多糖纯 品，检测其理化性质，并通过甲基化、GC-MS分析，初步确证糖链中羟乙基主要取 代在葡萄糖6位和困糖的1位上。药理实验表明，羟乙基化牛膝多糖对荷Lewis肺癌 小鼠NK活性具有一定促进作用。     

Hydroxyethylation of Achryranthes bidentata Polysaccharide and Investigation of Their Antitumor Activity

The hydroxyethylated Achyranthes bidentata polysaccharides (Abps) were synthesized. The distribution of substituents in these derivatives was studied using HPLC and NMR analysis. The results show that the substituents are not homogeneously distributed along the Abps chain and the reactivity of the hydroxyl groups in the sugar units is OH‐4 ? OH‐3>OH‐6≈OH‐1. Bioassay showed that the derivative with a low degree of substitution (DS) was the best choice to inhibit the growth of Lewis pulmonary carcinoma.     

吲哚丁酸对怀牛膝幼苗生长及谷胱甘肽抗氧化酶系统的影响

对怀牛膝幼苗施加不同浓度的吲哚丁酸(IBA),研究IBA对其幼苗生长及谷胱甘肽抗氧化酶系统的影响.结果显示:1.0mg/L IBA能同时显著提高怀牛膝叶片中谷胱甘肽过氧化物酶(GPx)、谷胱甘肽还原酶(GR)、谷胱甘肽S-转移酶(GST)的活性及蛋白质含量(P<0.05),并能促进其幼苗株高、根长和根干重的增加.结论:1.0mg/L IBA促进了牛膝幼苗的生长,提高了叶片中谷胱甘肽抗氧化酶系统的活性及蛋白质含量,有效的增强了植株的抗逆性.     

A Multi-Omics Analysis Reveals Anti-Osteoporosis Mechanism of Four Components from Crude and Salt-Processed Achyranthes bidentata Blume in Ovariectomized Rats

The root of Achyranthes bidentata Blume (AB) is a well-known traditional Chinese medicine for treating osteoporosis. Plenty of studies focused on the pharmacological mechanism of the whole extract; however, the contribution of different components to the anti-osteoporosis effect remains unknown. The aim of this study is to explore the anti-osteoporosis mechanism of different components of crude and salt-processed AB under the guidance of network pharmacology, metabolomics, and microbiomics. First, network pharmacology analysis was applied to constructing the compound-target-disease network of AB to provide a holistic view. Second, the anti-osteoporosis effects of the four components were evaluated in female Wistar rats. The subjects were divided into a normal group, a model group, a 17α-estradiol (E2)-treated group, a polysaccharide-component-treated groups, and a polysaccharide-knockout-component-treated groups. All the serum, urine, and feces samples of the six groups were collected after 16 weeks of treatment. Biochemical and microcomputed tomography (μCT) parameters were also acquired. Coupled with orthogonal partial least-squares discrimination analysis, one dimensional nuclear magnetic resonance (NMR) was used to monitor serum metabolic alterations. A total of twenty-two biomarkers, including lipids, amino acids, polyunsaturated fatty acids, glucose, and so on were identified for the different components-treated groups. Through pathway analysis, it is indicated that glyoxylate and dicarboxylate metabolism, glycine, serine, and threonine metabolism, alanine, aspartate, and glutamate metabolism, d-glutamine, and d-glutamate metabolism were the major intervened pathways. Levels of these biomarkers shifted away from the model group and were restored to normal after treatment with the four components. In addition, 16S rDNA sequencing demonstrated that the abundance of Anaerofilum, Rothia, and Turicibacter bacteria was positively correlated with an anti-osteoporosis effect, whereas the abundance of Oscillospira was negatively correlated. The osteoprotective effect of the polysaccharide components of crude and salt-processed AB is related to the regulation of the abundance of these gut microbiota.