摘花序和打顶对怀牛膝叶绿素荧光特性和品质的影响

以现蕾期怀牛膝为材料,研究了摘除全部花序、打顶等处理对怀牛膝生育性状特征、叶绿素含量、叶绿素荧光特性、根部产量和多糖含量的影响.结果表明,现蕾期摘除全部花序后,怀牛膝植株生长健壮,叶绿素含量升高,Fw/Fo,Fv/Fm和ETR升高.潜在光合性能增强,根部产量和多糖含量升高;打顶后植株生殖生长旺盛,根部增产效果不明显.     

怀牛膝对未孕大鼠子宫平滑肌峰电活动的影响

在 Wistar未孕大鼠子宫角浆膜面上埋植一对 Ag- Ag Cl双极电极 ,腹腔注射不同剂量怀牛膝水煎剂后观察大鼠在体子宫平滑肌峰电活动的改变 .结果显示 :怀牛膝水煎剂显著增加未孕大鼠子宫肌电爆发波的最大振幅和峰面积 (P <0 .0 5 ) ,可延长爆发波持续时间和增加爆发波动作电位个数 (P <0 .0 5 ) ;怀牛膝水煎剂明显增大未孕大鼠子宫肌电单波的正波最大振幅、负波最大振幅和峰面积 (P <0 .0 5 ) .怀牛膝显著兴奋在体子宫平滑肌峰电活动的效应类似催产素 .     

吲哚丁酸对怀牛膝幼苗生长及谷胱甘肽抗氧化酶系统的影响

对怀牛膝幼苗施加不同浓度的吲哚丁酸(IBA),研究IBA对其幼苗生长及谷胱甘肽抗氧化酶系统的影响.结果显示:1.0mg/L IBA能同时显著提高怀牛膝叶片中谷胱甘肽过氧化物酶(GPx)、谷胱甘肽还原酶(GR)、谷胱甘肽S-转移酶(GST)的活性及蛋白质含量(P<0.05),并能促进其幼苗株高、根长和根干重的增加.结论:1.0mg/L IBA促进了牛膝幼苗的生长,提高了叶片中谷胱甘肽抗氧化酶系统的活性及蛋白质含量,有效的增强了植株的抗逆性.     

链霉素对怀牛膝中钾、钙、铁元素含量的影响

以怀牛膝风筝棵和核桃纹2个品种为材料,研究了链霉素对怀牛膝中K,Ca,Fe元素含量的影响,原子吸收光谱法测定结果表明风筝棵和核桃纹中均含有K,Ca,Fe元素,且3种元素的含量差别非常明显,KCaFe.与对照相比,低浓度链霉素(50mg/L)处理使得怀牛膝中K,Ca,Fe元素含量增加,而高浓度链霉素(100mg/L)处理却使K,Fe元素含量降低.链霉素对风筝棵和核桃纹2个品种中K,Ca,Fe元素含量的影响程度不同,对风筝棵的影响程度较大.本研究结果表明,怀牛膝的两个品种风筝棵和核桃纹中的K,Ca,Fe元素含量有明显差别,且链霉素对其产生的影响也不同,链霉素对怀牛膝中微量元素影响的具体机制还不清楚,需要进一步研究.     

Achyranthes bidentata Blume extract promotes neuronal growth in cultured embryonic rat hippocampal neurons

We have prepared an aqueous extract of Achyranthes bidentata Blume, a commonly prescribed Chinese medicinal herb, and reported, in previous studies, that A. bidentata extract benefits nerve growth and prevents neuron apoptosis. In this study, we investigated the actions of A. bidentata extract on survival and growth of primarily cultured rat hippocampal neurons. The morphological observation revealed that neurite growth from hippocampal neurons was significantly enhanced by A. bidentata extract with similar effects to those induced by nerve growth factor (NGF), and the greatest neurite growth appeared on treatment with A. bidentata extract at 1 μg/ml for 24 h. DNA microarray analysis indicated that there were 25 upregulated genes and 47 downregulated genes exhibiting significantly differential expression in hippocampal neurons treated with A. bidentata extract at 1 μg/ml for 6 h when compared to those in untreated hippocampal neurons. Real-time quantitative RT-PCR and Western blot analysis demonstrated that the expression of growth-associated protein-43 in hippocampal neurons was upregulated at both mRNA and protein levels after treatment with A. bidentata extract, and the optimal dosage of the extract was also 1 μg/ml. These data confirm that A. bidentata extract could promote in vitro hippocampal neuronal growth in a dose- and time-dependent manner.     

怀牛膝愈伤组织诱导及分化的研究

对怀牛膝叶片和茎段愈伤组织的诱导及分化进行了研究.结果表明:KT 0.5 mg/L+2,4-D 1.5 mg/L对叶片愈伤组织的诱导效果最好,KT 0.5 mg/L+NAA 1.5 mg/L对茎段愈伤组织的诱导效果最好,出愈率均达100%;KT 0.5 mg/L+NAA 1.5 mg/L有利于叶片形成的愈伤组织分化为根,6-BA 0.5 mg/L+NAA 1.5 mg/L则有利于茎段形成的愈伤组织分化为芽.     

怀牛膝多糖对肺腺癌细胞系凋亡的调节

目的:研究怀牛膝多糖的抗肿瘤作用.方法:应用MTT法检测怀牛膝多糖在体外对肺腺癌细胞系(LTEP-a-2)增殖的调节,同时采用流式细胞术(FCM)检测其对LTEP-a-2细胞周期和凋亡的影响.结果:怀牛膝多糖对LTEP-a-2的增殖具有抑制作用,并可以诱导其凋亡.结论:怀牛膝多糖在体外能抑制肺癌细胞的生长,可作为一种抗肺癌药物.     

溶剂浮选法分离富集怀牛膝中总甾酮

应用溶剂浮选法于怀牛膝中甾酮的分离和富集,对溶剂浮选的条件(包括浮选溶剂的选择,浮选时溶液的酸度条件,通入氮气的流速及浮选过程所需时间等)作了研究和优化,所选定的优化条件如下:①选择正丁醇作为溶剂;②浮选时溶液酸度为pH 5;③通氮的最佳速率为40 mL·min-1;④浮选过程时间选定为80 min.用紫外分光光度法在247 nm波长处测定经浮选后正丁醇有机相吸光度,从而估量分离所得甾酮的量及浮选效率.经试验证明:与溶剂萃取法相比较,溶剂浮选法分离和富集怀牛膝中甾酮在效率及所需时间上具有明显的优越性.