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Using spatially modulated illumination (SMI) light microscopy it is possible to measure the sizes of fluorescent structures that have an extension far below the conventional optical resolution limit (“subresolution size”). Presently, the sizes are determined as the object extension along the optical axis of the SMI microscope. For this, however, “a priori” assumptions on the fluorochrome distribution (“shape”) within the examined fluorescent structure have to be made. Usually it is assumed that the fluorochrome follows a Gauss-distribution or a spherical distribution. In this report we overcome the necessity to make an assumption on the shape of the fluorochrome distribution. We introduce two new experimentally obtained parameters which allow the determination of a shape measure to describe the spatial distribution of the fluorescent dye. This becomes possible by independent measurements with different excitation wavelengths. As an example, we present shape parameter measurements on individual fluorescent microspheres with a nominal geometrical diameter (“size”) of 190 nm. In the case investigated, the experimental shape correlated well with a homogeneous fluorochrome distribution (“spherical shape”) but not with a variety of other “shapes”. 相似文献
3.
Endoscopic Observation of Tissue by Narrowband Illumination 总被引:6,自引:0,他引:6
Kazuhiro Gono Kenji Yamazaki Nobuyuki Doguchi Tetsuo Nonami Takashi Obi Masahiro Yamaguchi Nagaaki Ohyama Hirohisa Machida Yasushi Sano Shigeaki Yoshida Yasuo Hamamoto Takao Endo 《Optical Review》2003,10(4):211-215
We propose a new illumination method for a medical endoscope: narrow band imaging (NBI), in which the spectral bandwidth of the filtered light is narrowed. To confirm how the spectral specifications of the filtered light influence a reproduced image, an experiment was conducted observing the endoscopic images of the back mucosa of a human tongue. In addition, the effect of NBI on endoscopic images was investigated through preliminary clinical tests in colonoscopy and upper gastrointestinal endoscopy. It has been shown that NBI can enhance the capillary pattern and the crypt pattern on the mucosa. These patterns are useful features for diagnosing an early cancer. 相似文献
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Endoscopic illumination systems conduct light down a built-in fibre-optic bundle to illuminate the object. Many modern endoscopes employ wide-field imaging optical systems and therefore require an illuminating beam of large divergence. This is commonly provided by placing a strong diverging lens at the far end of the fibre bundle, but it results in a poor uniformity of illumination in the object space. This paper describes the design of a telescopic illumination system to overcome this problem. 相似文献
6.
Jos A. Gmez-Pedrero Juan A. Quiroga M. Jos Terrn-Lpez Daniel Crespo 《Optics and Lasers in Engineering》2006,44(12):1297-1310
In this paper we present the application of a direct demodulation method for the measurement of surface topography by means of Shadow-Moiré. In our set-up, we use three LEDs (with green, red and blue peak wavelengths) to illuminate the grating. Due to the different position of these light sources, a polychromatic Shadow-Moiré fringe pattern is produced, which can be described as the superposition of three monochromatic (red, green and blue) fringe patterns. Taking the image of this polychromatic fringe pattern with a RGB CCD camera, we get a monochromatic fringe pattern stored at each RGB channel of the CCD. The direct demodulation algorithm employed uses these fringe patterns to calculate the wrapped phase map. After unwrapping the phase map using a standard multi-grid technique, we implemented an automatic procedure to detect the area of interest of the phase map by removing low modulation zones and to calculate the absolute value of the phase. In this way it is possible to determine the topography of a surface with a single RGB snapshot maintaining a simple experimental set-up, which is an important feature, especially for the study of dynamic phenomena such as deformations. We present the experimental results obtained after measuring different objects with both smooth and rough surface textures. 相似文献
7.
According to the concept of the recognized visual space of illumination (RVSI) the lightness of an object surface is perceived in relation to its conceptualized size. To prove this proposition the lightness of gray test patches was judged when they were located at various positions inside an illuminated space composed of two rooms in the depth direction from a subject. No retinal image arrangement was changed in the test patch and its immediate surroundings, but the front room had walls, floors and furniture lower in lightness by the amount of N1.5 than the back room to make the RVSI of the former smaller despite the illuminance in the entire space being the same. The results showed that the apparent lightness of the patches was perceived higher by amount of about 13 in L units for the N4 test patch and about 20 for N6 when the patches were located in the front room, in accordance with the prediction. It was stressed that the experiment of lightness judgment should be conducted in a three dimensional space rather than two dimensional plane as done by several investigators. 相似文献
8.
The sensing light source of the line scan camera cannot be fully exposed in a low light environment due to the extremely small number of photons and high noise, which leads to a reduction in image quality. A multi-scale fusion residual encoder-decoder (FRED) was proposed to solve the problem. By directly learning the end-to-end mapping between light and dark images, FRED can enhance the image's brightness with the details and colors of the original image fully restored. A residual block (RB) was added to the network structure to increase feature diversity and speed up network training. Moreover, the addition of a dense context feature aggregation module (DCFAM) made up for the deficiency of spatial information in the deep network by aggregating the context's global multi-scale features. The experimental results show that the FRED is superior to most other algorithms in visual effect and quantitative evaluation of peak signa-to-noise ratio (PSNR) and structural similarity index measure (SSIM). For the factor that FRED can restore the brightness of images while representing the edge and color of the image effectively, a satisfactory visual quality is obtained under the enhancement of low-light. 相似文献
9.
《Organic Electronics》2014,15(1):299-305
A direct comparison of charge transport and electronic traps in representative polymer–fullerene blend, poly (3-hexylthiophene) (P3HT) and 1-(3-methoxycarbonyl)-propyl-1-phenyl-(6,6)C61 (PCBM), is carried out in dark and illuminated conditions based on the measurements of temperature-dependent current–voltage characteristics. In dark condition, the charge transport presents a transition from Ohmic to trap-limited current. While the trap-filled space charge limited current is observed under illumination at the same applied bias. From evaluations of trap density and energy distribution by a differential method, it is reveal that the diverse charge transport in dark and illuminated conditions is mainly caused by the different trap states distribution, which strongly affects the space charges and the electrical field in P3HT: PCBM blends. 相似文献
10.
A robust system for backlit keyboard inspection is revealed. The backlit keyboard not only has changeable diverse colors but also has the laser marking keys. The keys on the keyboard can be divided into regions of function keys, normal keys, and number keys. However, there might have some types of defects:incorrect illuminating area, non-uniform illumination of specified inspection region (IR), and incorrect luminance and intensity of individual key. Since the illumination features of backlit keyboard are too complex to inspect for human inspector in the production line, an auto-mated inspection system for the backlit keyboard is proposed in this paper. The system was designed into the operation module and inspection module. A set of image processing methods were developed for these defects inspection. Some experimental results demonstrate the robustness and effectiveness of the proposed system. 相似文献