Simultaneous quantification of leukotrienes and hydroxyeicosatetraenoic acids in cell culture medium using liquid chromatography/tandem mass spectrometry

Leukotrienes (LTs) and hydroxyeicosatetraenoic acids (HETEs) are important bioactive lipid mediators that participate in various pathophysiological processes. To advance understanding of the mechanisms that regulate these mediators in physiological and pathological processes, an analytical method using liquid chromatography/tandem mass spectrometry for the simultaneous quantification of LTB₄, LTC₄, LTD₄, LTE₄, 5‐HETE_, 8‐HETE, 12‐HETE and 15‐HETE in cell culture media was developed. A Supel?‐Select HLB solid‐phase extraction cartridge was used for sample preparation. The compounds were separated on a C₁₈ column using gradient elution with acetonitrile–water–formic acid (20:80:0.1, v/v/v) and acetonitrile–formic acid (100:0.1, v/v). The calibration curves of LTB₄, LTD₄, LTE₄ and HETEs were linear in the range of 0.025–10 ng/mL, and the calibration curve of LTC₄ was linear in the range of 0.25–10 ng/mL. Validation assessment showed that the method was highly reliable with good accuracy and precision. The stability of LTs and HETEs was also investigated. Using the developed method, we measured LTs and HETEs in the culture supernatant of the human mast cell line HMC‐1. The present method could facilitate investigations of the mechanisms that regulate the production, release and signaling of LTs and HETEs. Copyright © 2014 John Wiley & Sons, Ltd.     

全球宽带接入市场发展分析分析

通过对目前宽带线路数最多的10个国家和地区、2003年上半年新增线路最多的10个国家、全球宽带业务普及率最高的10个国家和地区、部分运营商住宅DSL业务的第一年费用以及2000～2002年全球入门级住宅DSL业务价格的发展趋势等图表的分析,阐明了全球宽带接入市场的发展状况,指出了其市场规模继续扩大、经济发达国家宽带线路增长速度较快、普及率的差距逐渐缩小以及业务价格趋于稳定等特点.     

通信性能评估的仿真实验研究

研究了流量控制和差错控制对通信性能的综合影响，着重分析了停止-等待协议和连续ARQ协议的优缺点，讨论了信道利用率和帧长的关系。介绍了几种多点接人技术的基本原理，如CSMA和CSMA／CD，并给出了各自相应的性能仿真曲线。所设计的通信性能评估实验，仿真界面形象丰富，与实验知识点紧密结合，可以大大提高实验教学的效率和质量。     

Scheduling Algorithms for Packet-Oriented MAC Protocols in Wireless Multimedia Systems

Future generation wireless multimediacommunications will require efficient Medium AccessControl (MAC) protocols able to guarantee suitable Qualityof Service (QoS) levels for different traffic classes whileachieving a high utilization of radio resources. This paperproposes a new scheduling technique to be adopted at the MAClevel in wireless access systems, named Dynamic Scheduling-Time DivisionDuplexing (DS-TDD), that efficiently managesvideo, voice, Web and background traffics. A theoretical approachis proposed in this paper to evaluate the DS-TDD performance withvoice and Web traffics. Simulation results have permitted tohighlight the following promising characteristics of the DS-TDDscheme: (i) a high capacity of real-time traffics isattained with a QoS insensitive to Web and background trafficloads; (ii) a high throughput can be guaranteed whilepreserving the QoS levels of the different traffic classes;(iii) heavier downlink traffic loads do not modify the QoSof uplink traffics. Finally, extensive comparisons with differentscheduling schemes proposed in the literature have permitted tohighlight the better performanceof DS-TDD.     

数字电视的条件接收系统

数字电视的兴起使人们能够欣赏到高质量的电视节目,伴随数字电视的成熟,条件接收系统(CAS)得到很好的发展。主要介绍数字电视条件接收系统相关技术的发展、基本功能、安全机制、工作原理及其安全性要求。     

创建互联网业务价值链

为促进互联网的良性发展,要使设备制造商、电信运营商、虚拟运营商/集团用户、内容提供商和最终用户构成一条互联网业务价值链,价值链的创建对互联网接入设备提出了更高的要求,华为公司的Quid-wayTMA8010Expert多业务综合接入平台可构建宽带城域网分组语音解决方案、可平滑演进为下一代网络中的媒体网关、提供窄带价值链、提供唯一号码业务以及实现多业务综合接入。     

一种实现最佳用户检测的非线性优化神经网络

本文提出并讨论了实现码分多址（CDMA）系统上最佳多用户检测（MUD）的一种神经网络方法。该方法通过将最佳多用户检测视为非线性优化组合问题，利用神经网络能有效求解非线性优化问题的优势，导出了一种非线性优化神经网络来实现最佳多用户检测，理论分析和计算机模拟表明，所提出的神经网络具有可实时应用的动态性能和较传统方法优越得多的误码率性能和抗多址干扰的性能。     

UHF频段卫星移动通信系统若干问题的探讨

本文根据我国陆海空移动用户的需求与特点，借鉴国外经验，提出了建设ＵＨＦ频段卫星移动通信系统的建议；对照已建成的Ｃ波段卫星固定通信系统，分析了其优缺点；最后深入探讨了应用中的若干技术问题。     

欧洲无线DSL的发展现状及市场前景

对欧洲的立法者来说，目前最为关注的课题之一便是如何解决数字鸿沟这一困扰欧洲大陆信息与通信技术整体发展水平的矛盾。向人们提供高速因特网接入服务是消除数字鸿沟的有效手段，而无线数字用户线技术以及其成低、通信质量好、覆盖范围大及灵活性等优势成为宽带接入技术中的佼佼者，并获得很多欧洲国家电信运营商的青睐。     

非关联双模场对Kerr介质中电子绝热跃迁转移现象的影响

本文研究了充满Kerr介质的高Q腔中非关联双模相干态场与级联三能级原子非共振相互作用情况下,电子的绝热跃迁转移现象与非关联场之间的关系。数值计算显示:级联三能级原子在与非关联双模相干态的相互作用下,该现象依然存在,但呈现不同于原子与关联场相互作用的新特征。