糖蛋白/胆红素钙水溶性复合物的形成及光谱研究

采用傅立叶变换红外光谱(FT-IR)，结合减法、二阶导、退卷积和曲线拟合等先进光谱技术，紫外可见光谱，ICP-AES分析方法，对不同质量比的α-酸性糖蛋白(AGP)和胆红素钙(CaBR)反应后的滤液进行了研究。结果表明，AGP和CaBR之间存在着较强的相互作用，导致AGP二级结构的有序性增加，CaBR的水溶性增大，两者形成了可溶性的AGP/CaBR复合物。     

磁疗手术胆结石的红外光谱观测

本文报道用Nicolet 170 SXFT-IR红外光谱仪观测磁疗手术胆结石、磁疗破碎排出胆结石及服中草药破碎胆结石的红外光谱,分析并比较了它们的光谱特征。     

磷脂水解与胆固醇型胆结石时的可能生成机理

用胆酸盐-磷脂-胆固醇-水体系模拟胆汗的化学行为。在体系中发现，磷脂可 以发生降解形成脂肪酸。而在胆汗中，磷脂在增溶固醇的过程中起到了十分重要的 作用。依此，我们提出磷脂的水解可能与胆固醇型胆结石的生成有密切关系。     

色素类胆结石难溶原因的分析

本文采用不同的溶剂对溶解前后的胆色素类结石进行了主要元素的控制，同时对特征色素类结石进行了有机元素分析，通过比较溶解前后超微结构和各种元素含量的变化，认为胆红素与金属离子，尤其是钙及其后二阶金属离子，形成了胆色素盐或配位络合物，并与糖蛋白等螯合成了大分子复合物。这是胆色素类结石难溶的主要原因。     

胆结石中蛋白质的傅里叶变换红外光谱和表面增强拉曼光谱研究

用氯仿、乙醚、乙醇和盐酸等溶剂溶解一组人体胆结石，获取难溶物；用傅里叶变换红外光谱和表面增强拉曼光谱对难溶剩余物进行研究。结果显示，结石难溶物主要由胆红素盐和蛋白质组成，结石中的蛋白质的二级结构以α螺旋和无规卷曲构象为主，其中α螺旋构象成分较多。讨论了蛋白质在胆结石形成中的作用。     

Investigation of Surface-Enhanced Raman Spectra of Human Gallstones on Colloidal Silver

The surface-enhanced Raman scatterihg (SERS) spectra of human gallstones is investigated. Complicated Raman bands were due to multiple components that include bilirubin, bilirubinate salts, cholesterol, fatty salts and proteins. The results suggest that besides bilirubin and cholesterol, fatty salts and proteins may play an important role in the formation of gallstone. The experimental data supply an useful information for the research of the formation mechanism of gallstones.     

胆结石的SEM和EDS研究

本文用电子显微镜对１５例胆固醇类结石和１５例胆色素类结石进行了超微结构的观察，并用能谱仪对原子序数大于１１的元素进行了分析，相对比较了用不同溶剂溶解后的胆色素类结石的微观结构和元素组成，同时还分析了胆色素类结石中碳，氢和氮的含量，根据以上结果，对色素类结石可能存在的物质及其成石机制进行了讨论。     

Investigation of Surface-Enhanced Raman Spectra of Human Gallstones on Colloidal Silver

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Nd:YAG激光器在治疗胆结石方面的应用与展望

重点介绍了激光碎石术的机理及Nd：YAG激光碎石机的发展状况,并根据我国现状,提出现阶段对于激光碎石机的研究重点放在降低成本,并指出只有提高碎石效率及安全性,才能使激光碎石机更加广泛地应用于结石病的治疗。     

Application of nonionic surfactants combining hydrophobic and hydrophilic cholelitholytic solvents on dissolution of gallstones

Application of nonionic surfactants in chemolitholysis was developed to combine two immiscible cholelitholytic solvents, MTBE and EDTA, into a homogeneous solution to increase the efficiency of gallstone dissolution. Eight kinds of Sinopol were employed to investigate and prepare the homogeneous solution, which included MTBE, EDTA (2wt.%) aqueous solution and the Sinopol, thereby, an in vitro study of chemolitholysis was carried out. Three kinds of gallstones were characterized by FTIR and SEM. The dissolution percentage of three kinds of gallstones in the homogeneous (Sinopol mixed) solution was evaluated and a comparison of the average dissolution percentage between these Sinopol mixed solutions and traditional cholelitholytic solvents was made. The composition of gallstones was classified into oil-soluble and water-soluble groups. Furthermore, the hydrophobic and hydrophilic properties of solvents corresponding to oil-soluble and water-soluble gallstone components were analysed and discussed with a phase diagram.