高频电离子手术联合爱宝疗浓缩液治疗尖锐湿疣的疗效观察

目的：探讨高频电离子手术联合爱宝疗浓缩液治疗尖锐湿疣的疗效；方法：对180例尖锐湿疣患者疣体分别用手术、高频电离子和电离子联合爱宝疗治疗，比较治疗有效率、复发率、创面出血和愈合情况。结果：电离子治疗组的有效率、复发率和创面出血发生率与手术治疗组相比有统计学差异，但创面不佳愈合的发生率高于联合治疗组（P＜0.05)。结论：高频电离子手术治疗方法是治疗尖锐湿疣较佳的方法，而且创面应用爱宝疗有助于愈合。     

CO2激光联合不同方法使用干扰素治疗尖锐湿疣疗效观察

目的观察CO 2激光联合不同方法使用干扰素治疗尖锐湿疣疗效.方法140患者随机分为四组，给予不同治疗，比较四组的复发率.结果A,B,C和D组的复发率分别是37.1%, 18.92%,9.4%和16.67%.两两比较有显著性差异.(P<0.05)结论CO 2激光联合重组人a-2b干扰素皮损内封闭能显著降低尖锐湿疣的复发.     

Determination of Camptothecin and 10-Hydroxycamptothecin in Camptotheca acuminata by LC-ESI-MS/MS

A rapid and sensitive liquid chromatography-tandem mass spectrometry method with multiple reaction monitoring was developed for the simultaneous determination of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata. The separation of camptothecin and 10-hydroxycamptothecin was performed on an Agilent Eclipse XDB-C18 column with a mixture of methanol and water (1:1, v/v) containing 0.2% formic acid as a mobile phase. The limits of detection of camptothecin and 10-hydroxycamptothecin were 4.0 ng/mL and 7.0 ng/mL (S/N = 3), respectively. Analysis took 10 minutes, making the method suitable for rapid determination of camptothecin and 10-hydroxycamptothecin in C. acuminata.     

宫颈湿疣临床诊治研究

目的探讨宫颈湿疣阴道镜下的诊断价值及激光治疗的疗效.方法自1999年9轮?000年9月间就诊于阴道镜门诊的177例患者均作阴道镜检察并作镜下活检.治疗方法CO2激光气化治疗.结果其中经临床诊断为宫颈尖锐湿疣的为43例,经阴道镜诊断为宫颈尖锐湿疣81例,扁平型湿疣80例,故宫颈湿疣的临床诊断检出率24.3%,漏诊率75.7%,阴道镜诊断检出率91.0%,漏诊率9%.在96例扁平型患者中,一次治愈74例(占77.1%),二次治愈20例(占20.8%),三次治愈2例(占2.1%).81例尖锐型患者中,一次治愈78例(占96.3%),二次治愈3例(占3.7%).177例宫颈湿疣,一次治愈率85.9%,二次治愈率98.9%,三次治愈率100%.结论阴道镜检查为诊断宫颈湿疣的一种敏感性高的诊断方法,CO2激光是一种安全,可靠,有效的治疗方法.     

5-氨基酮戊酸光动力疗法联合CO2激光治疗尖锐湿疣疗效的系统评价

目的：系统评价5-氨基酮戊酸光动力疗法（ALA-PDT）联合CO2激光与CO2激光单用比较治疗尖锐湿疣的疗效和安全性。方法：计算机检索CNKI、VIP、Google学术收索引擎和万方数据知识服务平台（2001至2011.11）,按照拟定的纳入及排除标准选择文献、提取资料和进行质量评价,采用RevMan 5.0软件对纳入文献进行Meta分析。结果：共纳入10个随机对照实验,合计841例患者。Meta分析结果表明,5-氨基酮戊酸光动力疗法联合CO2激光治疗复发率低于CO2激光治疗〔OR=0.15,95%CI（0.10,0.22）,P〈0.00001〕;治疗组治愈率高于对照组〔OR=6.93,95%C1（4.18,11.49）,P〈0.00001〕两种治疗方案产生的不良反应相当,差异无统计学意义〔OR=1.22,95%C1（0.42,3.59）,P=0.71〕。结论：5-氨基酮戊酸光动力疗法联合CO2激光治疗尖锐湿疣能有效降低复发率,提高临床治愈率。但由于纳入研究质量普遍较低,期待更多合理设计的随机对照实验提供高质量的证据。     

MOLECULAR CLONING OF A NEW VARIANT OF HUMAN PAPILLOMAVIRUS TYPE 6 ISOLATED FROM A CHINESE WOMAN AND EXPRESSION OF ITS L1 GENE IN E. coli——MOLECULAR CLONING & EXPRESSION OF HPV6 GENE

A human papillomavirus genome DNA of 7.9 kb from a Chinese woman with genital condyloma acuminata was cloned in Bam HI site of pAT153. According to the results obtained from Southern blotting, restriction mapping as well as partial DNA sequencing, the isolated genome (HPV6BV) had obvious variance and was referred to as a new variant of HPV6 found in China the first time. HPV6BV L1 gene was successfully expressed in E. coli as a fusion protein with pUR288. The β-galactosidase/L1 fusion protein reacted with both β-galactosidase antiserum and HPV antibody using Western blot technique. The E. coli-produced fusion protein, possessing HPV antigenicity, may provide a reagent for clinical diagnosis and epidemiological survey.     

喜树叶中喜树碱含量的高效液相色谱分析

建立了一种简便、快速、准确的喜树叶片中喜树碱含量的高效液相色谱分析方法 ;采用英国HPLCTechnology公司的TechsphereODS柱 (25cm×4.6mmID ,5μm) ,流动相乙腈 -水 (体积比4∶6) ,流速1.0mL·min -1,检测波长254nm ,柱温25℃ ,进样量10μL ;样品制备方法以61 % (φ)乙醇为溶剂 ,50℃下超声提取喜树叶粉10min;HPLC法测定喜树碱的含量 ;该法的RSD为2.5% (n=6) ,平均回收率为96 %。     

Neuropeltis acuminata (P. Beauv.): Investigation of the Chemical Variability and In Vitro Anti-inflammatory Activity of the Leaf Essential Oil from the Ivorian Species

The variability of chemical composition of the leaf essential oil (EO) from Neuropeltis acuminata, a climbing liana growing wild in Ivory Coast, was investigated for the first time. The in vitro anti-inflammatory activity was also evaluated. Thirty oil samples were isolated from leaves collected in three forests of the country and analyzed using a combination of Column Chromatography (CC), Gas Chromatography with Retention Indices (GC(FID)), Gas Chromatography-Mass Spectrometry (GC-MS), and ¹³Carbon-Nuclear Magnetic Resonance (¹³C-NMR). Fractionation by CC led to the first-time isolation from natural source of δ-cadinen-11-ol, whose structural elucidation by one dimension (1D) and 2D-NMR spectroscopy is reported here. Finally, 103 constituents accounting for 95.7 to 99.6% of the samples’ compositions were identified. As significant variations of the major constituents were observed, the 30 oil compositions were submitted to hierarchical cluster and principal components analyses. Five distinct groups were evidenced: Group I, dominated by (E)-β-caryophyllene, kessane, and δ-cadinene, while the main constituents of Group II were germacrene B, ledol, α-humulene, (E)-γ-bisabolen-12-ol, and γ-elemene. Group III exhibited guaiol, germacrene D, atractylone, (E)-γ-bisabolen-12-ol, δ-cadinene and bulnesol as main compounds. Group IV was dominated by (E)-nerolidol, guaiol, selina-4(15),7(11)-diene and bulnesol, whereas (E)-β-caryophyllene, α-humulene and α-muurolene were the prevalent compounds of Group V. As the harvest took place in the same dry season in the three forests, the observed chemical variability could be related to harvest sites, which includes climatic and pedologic factors, although genetic factors could not be excluded. The leaf oil sample S24 behaved as a high inhibitor of LipOXygenase (LOX) activity (half maximum Inhibitory Concentration, IC₅₀: 0.059 ± 0.001 mg mL⁻¹), suggesting an anti-inflammatory potential.     

激光治疗肛管-直肠下端尖锐湿疣28例分析

目的:本文用CO_2激光和Nd:YAG激光治疗肛管及直肠下端尖锐湿疣,并对分布、伴发部位、激光治疗方法及疗效进行分析。方法用2％利多卡因注射液局部浸润麻醉,CO_2激光直接气化,少数病人用卤化银CO_2光纤激光或Nd:YAG气化,激光气止血。位置较深的病人在肛门镜下进行。结果28例病人经1—5次治疗,总有效率96％,安全,可靠,无并发症。结论:激光治疗尖锐湿疣被公认为简便及有效的方法之一,对肛管及直肠下端的尖锐湿疣,配光纤的激光治疗更有其独特优越性,如经窥镜下对目不可及的病灶更为简便有效。     

CO2激光治疗尖锐湿疣的疗效观察及复发病例分析

观察CO2激光治疗尖锐湿疣(CA)的疗效,探讨术后复发的原因.经对临床确诊的146例CA患者进行192次治疗,结果表明,CO2激光治疗CA疗效确切,如能同时病因治疗和配合免疫增强剂、中医中药辩证治疗,可降低CA复发.