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1.
This study describes the development of a simple, enzyme-free, label-free, sensitive, and selective system for detecting adenosine based on the use of Tween 20-stabilized gold nanoparticles (Tween 20-AuNPs) as an efficient fluorescence quencher for boron dipyrromethene-conjugated adenosine 5′-triphosphate (BODIPY-ATP) and as a recognition element for adenosine. BODIPY-ATP can interact with Tween 20-AuNPs through the coordination between the adenine group of BODIPY-ATP and Au atoms on the NP surface, thereby causing the fluorescence quenching of BODIPY-ATP through the nanometal surface energy transfer (NSET) effect. When adenosine attaches to the NP surface, the attached adenosine exhibits additional electrostatic attraction to BODIPY-ATP. As a result, the presence of adenosine enhances the efficiency of AuNPs in fluorescence quenching of BODIPY-ATP. The AuNP-induced fluorescence quenching of BODIPY-ATP progressively increased with an increase in the concentration of adenosine; the detection limit at a signal-to-noise ratio of 3 for adenosine was determined to be 60 nM. The selectivity of the proposed system was more than 1000-fold for adenosine over any adenosine analogs and other nucleotides. The proposed system combined with a phenylboronic acid-containing column was successfully applied to the determination of adenosine in urine.  相似文献   
2.
张荣  曲宏伟 《微电子学》1998,28(6):437-439
制作压力传感器时,在二氧化硅层上淀积多晶硅膜,既可利用优良的机械特性,又可保证压敏电阻与衬底间具有良好的绝缘性,由此可大大提高器件的温度特性。介绍了一种多晶硅压力传感器的原理和设计。实验结果表明,这类传感器具有灵敏度好,精度高等特点,电路工作范围为0-250℃,且具有良好的温度稳定性。  相似文献   
3.
钢管内径光电检测装置   总被引:3,自引:0,他引:3  
马宏  宋路 《半导体光电》1996,17(3):261-266
介绍了一种检测细长钢管内径的光电检测装置。该装置利用线阵CCD作传感器,采用8031单片机作为主控制。  相似文献   
4.
A novel measurement method of temperature based on the phenomena that the phase difference between principle polarization states in the optical retarder is function of temperature is described. The polarization state of optical beam is changed as it passes through the optical retarder, which depends on the temperature. The temperature of optical retarder is determined by comparison of the power difference between principal polarization states. We demonstrate successfully the temperature measurement by using a polarization maintaining fiber as the optical retarder. With a 100 mm length of the fiber optic retarder, the change rate of phase difference on temperature was 0.236 rad/°C and the measurement error was ±0.038°C over the temperature range of −2.6 – +3.4°C. With a 11.5 mm length of the fiber optic retarder, the change rate of phase difference on temperature was 0.021 rad/°C and the measurement error was ±0.79°C over the temperature range of −8.5 – +86.5°C.  相似文献   
5.
基于SDB/SOI材料和硅微机械加工技术,提出了一种含微参比电极和择优差分补偿单元的背面引线PH-ISFET/压力传感器的新结构。并且设计了高稳定的可调芯片自恒温系统,以减少硅材料对温度敏感的效应。这种结构既方便地实现敏感元和信号处理电路的完全隔离,又有效地改善了敏感特性和稳定性。初步实验结果证实了新结构设计是成功的。  相似文献   
6.
液晶微位移传感器   总被引:2,自引:0,他引:2  
史永基 《半导体光电》1994,15(3):259-262
液晶微位移传感器的工作原理是以负介电各向异性的向列相液晶材料的动态散射效应为基础的,文中给出了这种传感器的结构,测量方法、精度、应用范围和实验结果;研究了在阈值电压附近光的吸收和散射,讨论了该传感器的稳定性和遥控方法。  相似文献   
7.
吴古棣 《电讯技术》1993,33(2):62-68
本文介绍国外战场雷达的现状,包括轻小型化、武器定位及低空目标探测等几个方面。  相似文献   
8.
微量稀土加入铝或铝合金中,如果加入方法、数量、时间得当,可明显改善材料的多项性能.稀土对铝及铝合金的影响规律和作用机理,已取得了一些进展,但对稀土铝合金的热力学性质,尤其是溶解态稀土的研究少有报导[‘,2].本工作采用L即.95C。。。。FZ。。多晶作为固体电解  相似文献   
9.
Song Z  Lü J  Zhao T 《Talanta》2001,53(6):2510-1177
A novel chemiluminescence (CL) sensor for isoniazid combined with flow-injection technology is presented in this paper. The analytical reagents, luminol and ferricyanide, were both immobilized on an anion-exchange column. The CL signal produced by the reaction between luminol and ferricyanide, which were eluted from the column through sodium phosphate injection, was decreased in the presence of isoniazid. The decreased CL intensity was linear with isoniazid concentration in the range 0.001–1.0 μg·ml−1; and the detection limit was 0.35 ng·ml−1 (3s). The whole process, including sampling and washing, could be completed in 2 min with a relative standard deviation of less than 4.1%. The sensor could be reused more than 400 times and has been applied for the determination of isoniazid in pharmaceutical preparations.  相似文献   
10.
This paper introduces a mathematical model which makes it possible both to determine the concentration of photosynthetic herbicides and to obtain a quantitative parameter in order to compare their activity using a previously described sensing system. The working principle involves the changes in absorption properties at 860 nm of the reaction centre (RC) isolated from the bacteria Rhodobacter sphaeroides when photosynthetic herbicides are present. The method has been used for the determination and activity comparison of five photosynthetic herbicides: diuron, atrazine, terbutryn, terbuthylazine and simazine. Detection limits obtained were 2.2, 0.75, 0.046, 0.25, and 1.4 μM, respectively. The resulting order for the different herbicides according to their action on RC was: terbutryn > terbuthylazine > atrazine > simazine > diuron.  相似文献   
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