A dried blood spot assay with HPLC–MS/MS for the determination of larotrectinib in mouse blood and its application to a pharmacokinetic study

Larotrectinib is a first-generation tropomyosin kinase inhibitor, approved for the treatment of solid tumors. In this paper, we present a validated dried blood spot (DBS) method for the quantitation of larotrectinib from mouse blood using HPLC–MS/MS, which was operated under multiple reaction monitoring mode. To the DBS disc cards, acidified methanol enriched with internal standard (IS; enasidenib) was added and extracted using tert-butyl methyl ether as an extraction solvent with sonication. Chromatographic separation of larotrectinib and the IS was achieved on an Atlantis dC₁₈ column using 10 mm ammonium formate–acetonitrile (30:70, v/v) delivered at a flow-rate of 0.80 ml/min. Under these optimized conditions, the retention times of larotrectinib and the IS were ~0.93 and 1.37 min, respectively. The total run time was 2.50 min. Larotrectinib and the IS were analyzed using positive ion scan mode and parent–daughter mass to charge ion (m/z) transitions of 429.1 → 342.1 and 474.1 → 267.1, respectively, were used for the quantitation. The calibration range was 1.06–5,080 ng/ml. No matrix effect or carryover was observed. Hematocrit did not influence DBS larotrectinib concentrations. All of the validation parameters met the acceptance criteria. The applicability of the validated method was shown in a mouse pharmacokinetic study.     

A review of computational modeling and deep brain stimulation: applications to Parkinson's disease

Biophysical computational models are complementary to experiments and theories, providing powerful tools for the study of neurological diseases. The focus of this review is the dynamic modeling and control strategies of Parkinson's disease (PD). In previous studies, the development of parkinsonian network dynamics modeling has made great progress. Modeling mainly focuses on the cortex-thalamus-basal ganglia (CTBG) circuit and its sub-circuits, which helps to explore the dynamic behavior of the parkinsonian network, such as synchronization. Deep brain stimulation (DBS) is an effective strategy for the treatment of PD. At present, many studies are based on the side effects of the DBS. However, the translation from modeling results to clinical disease mitigation therapy still faces huge challenges. Here, we introduce the progress of DBS improvement. Its specific purpose is to develop novel DBS treatment methods, optimize the treatment effect of DBS for each patient, and focus on the study in closed-loop DBS. Our goal is to review the inspiration and insights gained by combining the system theory with these computational models to analyze neurodynamics and optimize DBS treatment.     

Ir（Ⅳ）—KBrO3—DBS—偶氮氯膦催化分光光度法测定微量铱的研究

ＫＢｒＯ３－ＤＢＳ偶氮氯膦在０．８ｍｌ．Ｌ＾－１的Ｈ２ＳＯ４介质中长时间加热亦无明显褪色现象，但在有微量铱存在时，褪色反应在３ｍｉｎ内完成，且与Ｉｒ（Ⅳ）的浓度有良好的线性关系，据此建立了微量铱的催化褪色分光光度分析法，其检出限为０．０１μｇ．１０ｍＬ＾－１铱的含量在０．０１－０．２０μｇ．１０ｍＬ＾－１范围内符合比尔定律。     

Broadcasting by satellites

As a result of recent advances in signal processing technology and standards for video compression systems there has been a dramatic increase in interest in commercial applications. This paper reviews the current broadcasting situation from a number of viewpoints relevant to the future development of the available technologies. After a review of the context, which includes alternative new broadcasting technologies as well as alternative media capable of competing with satellite, digital technology is given a wide ranging assessment with particular attention paid to the direct broadcasting to the home by satellite application currently receiving considerable attention world-wide. The possible system architectures and subsystem design features are highlighted with the European Baseline Satellite Broadcasting specification developed by the European Digital Video Broadcasting Project gaining special attention. For completeness a brief review of the digital audio broadcasting system (DAB) is also given. In addition to the applications for direct broadcasting, applications in the professional domain for distributing television signals from studios to terrestrial transmitters, satellite up-link sites or cable heads or for satellite news gathering (SNG) are examined. Features of existing commercially available hardware to support these services are described briefly.     

双包层EYDFA增益钳制效果的理论研究

基于速率方程的离散算法,实现了环形腔控制方案下,双包层EYDFA增益钳制的分析,研究了单、多信道情况下增益、噪声指数钳制的效果。结果表明：对于单信道EYDFA,随着衰减器衰减系数的增大,EYDFA的增益钳制范围增大,但信号增益钳制的幅度减小,且信号噪声指数增大;对于多信道EYDFA,只要激光谐振不被破坏,各信道增益都能得到有效钳制。     

毫米波测云雷达回波信号衰减补偿仿真研究

针对毫米波测云雷达受云雨衰减的问题,从雷达I、Q信号角度出发,模拟毫米波测云雷达回波I、Q信号并假设一定的探测环境,进行雷达回波信号衰减补偿仿真试验.试验结果表明,雷达电磁波信号穿过雨区和云区时,在远距离处衰减量很大,通过衰减补偿,信号强度明显增强,目标信号突出.     

DBS成像技术在毫米波雷达导引头中的应用

本文介绍了DBS技术的基本概念及工作原理,并且结合毫米波雷达回波信号的特点,研究适合快速实时成像、且具有实际工程意义的DBS成像算法——SPECAN算法及其实现流程。在理论分析的基础上,给出了系统仿真的结果和性能参数,证明了理论的可行性,为实际工程上的应用打下了基础。     

卫星系统新技术--应用机遇及发展策略考虑(上)

结合市场导向下的技术驱动重要性，重点论述现代卫星系统新技术及其在中国的应用机遇和发展策略。     

匀加速运动平台下的大斜视DBS成像方法

 距离走动校正和二次相位误差补偿是匀加速运动平台下大斜视DBS成像的难点.因此,本文提出一种基于Radon变换校正距离走动、基于Radon模糊变换补偿二次相位误差的DBS成像算法,并推导了此校正和补偿算法的空域适应性条件.该算法原理简单、精度高,且无需雷达平台运动参数、视角等先验信息,可实现预定方位分辨率的DBS成像.仿真实验验证了算法的有效性.     

有线电视网络数字化进程中的几点思考

指出面对有线电视数字化 ,网络公司面临的几方面问题 ,提出目前应当着手进行的工作 ,同时对未来将会出现的问题进行简要分析并提出应对措施。