A statistical model for global optimization by means of select and clone

A statistical model tor giobai optimization is constructed generalizing some properties ofthe Wiener process to the multidimensional case. A new approach, which is similar to the Branch and Bound approach, is proposed to the construction of algorithms based on statistical models. A two dimensional version of the algorithm is implemented, and test results are presented     

The join of two minimal clones and the meet of two maximal clones

We construct two minimal clones on any finite set such that the join of the two clones contains all operations. Dually, we exhibit two maximal clones on any finite set with at least three elements such that the intersection of the two clones is the trivial clone containing projections only. Received October 20, 1998; accepted in final form December 16, 1998.     

Construction and Expression of Human Survivin and Preparation of Its Polyclonal Antibody

Survivin, a novel member of inhibitor of apoptosis（IAP） protein family, is aberrantly expressed in cancer but undetectable in normal, differentiated adult tissues. The cancer-specific expression of survivin, coupled with its importance in inhibiting cell death and in regulating cell division makes it a useful diagnostic marker of cancer and a potential target for cancer treatment. Survivin cDNA amplified from the total RNA of 293 cells through RT-PCR was cloned into prokaryotic expression vector pRSET-B. The recombinant plasmid pRSET-B-Surv was expressed in E.coli BL21, and the relative molecule mass（Mr） of expressed fusion protein was approximately 21000. The recombinant protein was purified through Ni^2＋ affinity chromatography column and characterized by SDS-PAGE and Western blot. The purified recombinant protein was then injected into rabbits, and antisurvivin polyclonal antibody with a high titer was obtained.     

基于免疫克隆算法的认知无线电多信道联合感知方法

该文研究了认知无线电多信道联合感知的数学模型,并将此模型转换为以认知系统总吞吐率最大化为目标的带约束优化问题,进而提出一种基于免疫克隆的多信道联合感知算法。仿真实验表明所提算法在收敛速度以及稳定性上均明显优于已有的基于遗传算法的感知方法,并且所提算法在对主用户造成相同的干扰条件下能提供更高的系统总吞吐率。     

Method for clone checking

A new method for simple and fast clone checking is described. We combined the Pyrosequencing technology with a preprogrammed nucleotide dispensation strategy for fast analysis of DNA constructs. To test this method, the N-terminus region of plasmids constructed for the production of recombinant apyrase was analyzed. Of the ten plasmids tested, seven constructs were correct, two constructs showed one base deletion, and one construct showed deletion of a 195 bp fragment. The preprogrammed nucleotide dispensation strategy allowed the identification of the sequence downstream of the deletions. Thus, this method determines both the location and nature of possible artifacts.     

Evaluation of thermal behavior of latex membranes from genetically improved rubber tree (Hevea brasiliensis)

Thermal stability, thermal decomposition process, residual mass, temperature of glass transition (T_g) and temperature dependence of storage modulus (E’), were determined for latex membranes prepared from six clones of Hevea brasiliensis: IAC 331, IAC 332, IAC 333 and IAC 334 grown at experimental plantations of Instituto Agronômico de Campinas (IAC) in Votuporanga, São Paulo State, Brazil. Latex membranes from GT1 and RRIM 600 Asian matrix clones were used as references. The thermal behavior of latex membranes from genetically improved rubber trees was characterized using thermogravimetry/derivative thermogravimetry (TG/DTG), differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMA). The thermal behavior of latex from clones studied in the present work showed similar features of the clones previously reported (IAC 40, IAC 300, IAC 301, IAC 328, IAC 329 and IAC 330), with mass loss in four consecutive steps, except IAC 333, which showed an additional mass loss step.     

On clones, transformation monoids, and finite Boolean algebras

We prove that, for a finite Boolean algebra, there exist only finitely many clones which consist of polynomial functions of the algebra and contain the monoid of its unary polynomial functions. Received January 7, 2000; accepted in final form December 13, 2000.     

基于免疫克隆选择算法的图像分割

图像分割是图像处理领域中不可缺少的一个分支。该文基于阈值分割方法,将免疫克隆选择优化算法应用到图像分割中,提出了一种新的图像分割算法。详细叙述了算法机理,并对算法复杂度进行了理论分析以及实验数据比较。在仿真实验中,将遗传算法和免疫克隆选择算法分别独立运行10次,对10次得到的阈值以及均值、方差进行了比较,并将函数评价次数作为算法复杂度的评价指标。该文算法不仅能够对图像进行准确的分割,而且在同样的种群规模下能够以较少的迭代代数和较低的函数评价次数得到最优阈值。仿真结果表明,该方法应用在图像分割中是可行的、有效的。     

SPAP2基因的克隆、融合表达及分离纯化

本文构建的表达载体pGex-2T-SPAP2CT在大肠杆菌中表达出可融性蛋白, 其分子量为46 000, 经纯化后得到产率为10%、 纯度大于90%的GST-SPAP2CT蛋白.     

A Generalized Version of the Baker–Pixley Theorem

A famous theorem by Baker and Pixley states that the term functions of a finite algebra with a (d + 1)-ary near-unanimity term are precisely the functions under which all subalgebras of the algebra's dth power are closed. In this paper, we generalize the theorem to a completely abstract level. Indeed, we obtain a version of the theorem that is stated in purely category-theoretic terms, making it applicable in any concrete or abstract category. To motivate this rather abstract result, we also discuss some of its concrete applications.