A simple and efficient one-pot synthesis of 1H-benzimidazole derivatives using thiamine hydrochloride (VB1) as the organocatalyst from o-phenylenediamine and aldehyde in dimethylformamide is described. Compared to classical reaction conditions, this new method consistently has the advantages of excellent yields, metal-ion-free procedure, and good recovery and reusablity of catalyst. 相似文献
Abstract An efficient and convenient protocol of broad scope for the synthesis of quinoxaline by cyclization-oxidation of phenacyl bromide with equimolar amount of phenylenediamine at room temperature catalyzed by thiamine in the form of N-heterocyclic carbene (NHC). High efficiency, inexpensiveness, and non-toxicity are the interesting features of the catalyst, which make it eco-friendly and highly attractive. 相似文献
Abstract Cd2+ ion was used as an electrochemical indicator to detect VB1 or Vc using square ware voltammetry (SWV) at a mercury film‐coated glassy carbon (GC) electrode. At pH=10 NH3‐NH4Cl buffer, a new cathodic peak was found at ?0.360 V (vs.SCE) by addition of thiamine, and the peak current of SWV was linear with the concentration of thiamine in the range of 1×10?6 to 4×10?3 M. On the other hand, the SWV peak current of Cd2+ at ?0.856 V linearly decreased with addition of ascorbic acid in the range of 6×10?6~10?3 M. The effects of interference, such as citric acid, DL‐malic acid, and calcium panlothenate, on thiamine or ascorbic acid determination were investigated. This method was successfully applied to the determination of thiamine or in pharmaceutical preparation. 相似文献
Abstract The potential of the continuous addition of reagent (CAR) technique, developed for reactions on the millisecond time scale, in fluorimetric reaction-rate methods, is evaluated. The approach was applied to the classical fast reaction involving the oxidation of thiamine to fluorescent thiochrome effected by potassium ferricyanide in basic solutions. Practical problems associated with this system were readily avoided by using the CAR technique. Several kinetic methods have been developed for the determination of thiamine; all are very sentitive (detection limit, 2. 5x10?9 M) compared to other fluorimetric equilibrium and reaction-rate methods and are suitable for routine analyses (sample throughput, 100 h?1). 相似文献
RNA Lego : The use of natural riboswitch aptamers in synthetic RNA switches (see picture) should broaden the scope of artificial RNA regulators dramatically. It is shown that thiamine pyrophosphate (TPP) aptamers can be used in engineered devices as very sensitive switches of gene expression in unmodified organisms. The approach demonstrates that intrinsic metabolites can be utilized as external effectors of cellular functions.
The x-ray crystal structure of thiamine bromide hydrate was studied. The crystals are monoclinic, a = 12.348, b = 24.852, c = 11.682 ,= 113.67°, Z = 4, space group P21/c. The crystal structure is constructed of two crystallographically independent thiamine cations, bromide anions, and water molecules. The thiamine cations have the F-conformation. The structure contains intermolecular short contacts involving S and N atoms of the thiazole ring.相似文献