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对短程飞行时间法(tim e-of-flight,TOF)中推算冷原子温度的理论拟合公式与近似拟合公式进行了误差分析与比较。研究表明:对于使用短程飞行吸收光谱信号推测冷原子团温度,当探测光光斑半径与冷原子团高斯半径之比k小于0.2时,理论拟合公式和近似拟合公式能很好的相符,随着探测光光斑半径与冷原子团高斯半径比值的逐渐增大,用近似拟合公式所得TOF吸收信号与用理论拟合公式所得TOF吸收信号的误差也将逐渐增大,当比值为0.5时,用近似拟合公式所得TOF吸收信号的误差将增大到20%。 相似文献
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Dr. Alexander Kyrychenko Prof. Dr. Alexey S. Ladokhin 《Chemical record (New York, N.Y.)》2024,24(2):e202300232
Fluorescence spectroscopy provides numerous methodological tools for structural and functional studies of biological macromolecules and their complexes. All fluorescence-based approaches require either existence of an intrinsic probe or an introduction of an extrinsic one. Moreover, studies of complex systems often require an additional introduction of a specific quencher molecule acting in combination with a fluorophore to provide structural or thermodynamic information. Here, we review the fundamentals and summarize the latest progress in applications of different classes of fluorescent probes and their specific quenchers, aimed at studies of protein folding and protein-membrane interactions. Specifically, we discuss various environment-sensitive dyes, FRET probes, probes for short-distance measurements, and several probe-quencher pairs for studies of membrane penetration of proteins and peptides. The goals of this review are: (a) to familiarize the readership with the general concept that complex biological systems often require both a probe and a quencher to decipher mechanistic details of functioning and (b) to provide example of the immediate applications of the described methods. 相似文献
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介绍采用短程飞行时间吸收谱测量铯原子磁光阱(MOT) 中冷原子温度的基本原理及实验实现.与通常的飞行时间方法不同,采用短程飞行时间吸收谱来测量MOT 中冷原子云的温度.在MOT 区域正下方若干毫米处入射一束圆柱状共振探测光束(实验中对于h=3mm,5mm,8mm的情况均作了研究),释放冷原子云,在其膨胀和自由下落过程中穿过探测光束,即可由光电探测器测得飞行时间吸收谱,由此推得MOT中冷原子的温度.
关键词:
磁光阱
冷原子
飞行时间
短程飞行时间
铯原子 相似文献
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本文列举了一些常规的和先进的核磁共振技术在药物天然产物研究中的应用,指出核磁共振波谱在天然化合物结构测定以及其他有关化学研究中起着重要作用。 相似文献
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The recording of digital hologram at short distance and reconstruction using convolution approach 下载免费PDF全文
By adopting in-line lensless Fourier setup and phase-shifting
technique, we recorded the phase-shifting digital hologram at short
distance. As the Fresnel diffraction condition is no longer valid,
the convolution approach is chosen for the reconstruction. However,
the simulated reference wave for the reconstruction would suffer
from severe under-sampling due to the comparatively large pixel
size. To solve this problem, sinc-interpolation is introduced to get
the pixel-size of the hologram reduced prior to the reconstruction.
The experimental results show that an object image of high fidelity
is obtained with this method. 相似文献
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