The in vitro Test for Pyrogens in Radiopharmaceuticals

The procedure and results of determination of pyrogenic substances in radiopharmaceutical preparations by in vitro method based on the reaction between bacterial endotoxine and Limulus a Amebocyte Lysate (LAL) have been presented in the paper. The advantage of this method as compared to the test in experimental animals performed so far has also been analyzed and proved by the fact that it enables avoidance of introduction of radioactivity in experimental animals and radiation effect on the results obtained for efficiency of investigated preparations. The in vitro method is also quick to perform and requires only small quantities of used radiopharmaceutical preparation.