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用电桥研究光敏电阻的光电特性 总被引:2,自引:1,他引:1
利用平衡电桥和非平衡电桥可以研究光敏电阻的光电特性,该实验的原理涵盖电学、光学、半导体物理学等基础知识.通过实验学生可认识到光敏材料的光电特性,并学会一些基本仪器的组装和调试,掌握一种将非电量转化为电量进行动态测量的方法. 相似文献
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A Phosphole Oxide Based Fluorescent Dye with Exceptional Resistance to Photobleaching: A Practical Tool for Continuous Imaging in STED Microscopy
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Dr. Chenguang Wang Prof. Dr. Aiko Fukazawa Prof. Dr. Masayasu Taki Dr. Yoshikatsu Sato Prof. Dr. Tetsuya Higashiyama Prof. Dr. Shigehiro Yamaguchi 《Angewandte Chemie (International ed. in English)》2015,54(50):15213-15217
The development of stimulated emission depletion (STED) microscopy represented a major breakthrough in cellular and molecular biology. However, the intense laser beams required for both excitation and STED usually provoke rapid photobleaching of fluorescent molecular probes, which significantly limits the performance and practical utility of STED microscopy. We herein developed a photoresistant fluorescent dye C‐Naphox as a practical tool for STED imaging. With excitation using either a λ=405 or 488 nm laser in protic solvents, C‐Naphox exhibited an intense red/orange fluorescence (quantum yield ΦF>0.7) with a large Stokes shift (circa 5900 cm?1). Even after irradiation with a Xe lamp (300 W, λex=460 nm, full width at half maximum (FWHM)=11 nm) for 12 hours, 99.5 % of C‐Naphox remained intact. The high photoresistance of C‐Naphox allowed repeated STED imaging of HeLa cells. Even after recording 50 STED images, 83 % of the initial fluorescence intensity persisted. 相似文献
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