Zn、Mg、FT3、FT4在甲亢患者血清中的含量及相关性探讨

测定了３１例甲状腺机能亢进患者血清中Ｚｎ,Ｍｇ和Ｔ３,Ｔ４含量,并对其临床意义及相关性进行了探讨,同时作正常人对照,经统计学处理。结果表明：Ｍｇ明显降低（Ｐ〈０．０１）,Ｚｎ明显升高（Ｐ〈０．０１）,ＦＴ３,ＦＴ４明显升高（Ｐ〈０．０５）,但此两种元素含量与ＰＴ３,ＰＴ４水平没有等级相关性。     

Optimization of Total Thyroxine Radioimmunoassay in Unextracted Serum by Dextran Charcoal

Specific method for accurate radioimmunoassay of free and total thyroxine in un-extracted serum has been developed. The method is used for estimation of either free thyroxine (T₄) or total T₄ by inhibition of the binding sites of T₄ to protein using TCA/NaOH mixture. The antibodies used in this study were obtained from Mallinkrodt. The separation technique used in our modification is that of dextran charcoal, this is to absorb the free fractions on charcoal.

The kinetics of antibody antigen combination, the parameters of the experiment like; charcoal concentration, antibody dilution, temp., time of incubation and radioactivity concentration were, studied to optimize the standard curve and the sensitivity of T₄RIA.

Serum samples of different thyroid cases were investigated for determination of free and total T₄ concentration to get the levels for each group of which 50 normals, 57 hyperthyroidism and 21 hypothyroidism in comparison with the classical tests of thyroid functions. Also sensitivity and variance coefficient has been carried out in comparison with other T₄RIA techniques.     

Reduced System Complexity of Heart Rate Dynamics in Patients with Hyperthyroidism: A Multiscale Entropy Analysis

Studying heart rate dynamics would help understand the effects caused by a hyperkinetic heart in patients with hyperthyroidism. By using a multiscale entropy (MSE) analysis of heart rate dynamics derived from one-channel electrocardiogram recording, we aimed to compare the system complexity of heart rate dynamics between hyperthyroid patients and control subjects. A decreased MSE complexity index (CI) computed from MSE analysis reflects reduced system complexity. Compared with the control subjects (n = 37), the hyperthyroid patients (n = 37) revealed a significant decrease (p < 0.001) in MSE CI (hyperthyroid patients 10.21 ± 0.37 versus control subjects 14.08 ± 0.21), sample entropy for each scale factor (from 1 to 9), and high frequency power (HF) as well as a significant increase (p < 0.001) in low frequency power (LF) in normalized units (LF%) and ratio of LF to HF (LF/HF). In conclusion, besides cardiac autonomic dysfunction, the system complexity of heart rate dynamics is reduced in hyperthyroidism. This finding implies that the adaptability of the heart rate regulating system is impaired in hyperthyroid patients. Additionally, it might explain the exercise intolerance experienced by hyperthyroid patients. In addition, hyperthyroid patients and control subjects could be distinguished by the MSE CI computed from MSE analysis of heart rate dynamics.     

Proteomics Profiling of the Urine of Patients with Hyperthyroidism after Anti-Thyroid Treatment

Hyperthyroidism, which is characterized by increased circulating thyroid hormone levels, alters the body’s metabolic and systemic hemodynamic balance and directly influences renal function. In this study, the urinary proteome of patients with hyperthyroidism was characterized using an untargeted proteomic approach with network analysis. Urine samples were collected from nine age-matched patients before and after carbimazole treatment. Differences in the abundance of urinary proteins between hyperthyroid and euthyroid states were determined using a 2D-DIGE coupled to MALDI-TOF mass spectrometry. Alterations in the abundance of urinary proteins, analyzed via Progenesis software, revealed a statistically significant difference in abundance in a total of 40 spots corresponding to 32 proteins, 25 up and 7 down (≥1.5-fold change, ANOVA, p ≤ 0.05). The proteins identified in the study are known to regulate processes associated with cellular metabolism, transport, and acute phase response. The notable upregulated urinary proteins were serotransferrin, transthyretin, serum albumin, ceruloplasmin, alpha-1B-glycoprotein, syntenin-1, and glutaminyl peptide cyclotransferase, whereas the three notable downregulated proteins were plasma kallikrein, protein glutamine gamma-glutamyl transferase, and serpin B3 (SERPINB3). Bioinformatic analysis using ingenuity pathway analysis (IPA) identified the dysregulation of pathways associated with cellular compromise, inflammatory response, cellular assembly, and organization and identified the involvement of the APP and AKT signaling pathways via their interactions with interleukins as the central nodes.