Transport engineering in microbial cell factories producing plant-specialized metabolites

Transport engineering strategies use altered expression of transporter proteins to change metabolite distribution within an organism. The production of plant specialized metabolites in microbial cell factories encounters a set of challenges that could benefit from the implementation of transport engineering technology. The range of challenges includes premature pathway termination due to secretion of intermediates, feedback inhibition due to inefficient export of final products, low yields in bioconversion processes due to inefficient import of precursors, and poor connectivity between subcellular compartments expressing different parts of complex biosynthetic pathways. We highlight the latest examples of transport engineering in microbial cell factories producing plant specialized metabolites, identify the current knowledge gap, and propose future research for advancing the field.     

细胞工厂与生物炼制

生物炼制是以可再生生物质资源为原料,生产能源与化工产品的新兴工业模式。是转变经济增长模式,保障社会经济可持续发展的重大战略需求。微生物细胞工厂是生物炼制技术至关重要的核心。世界各国纷纷设立重大研究计划支持细胞工厂的研究,以期获得生物炼制技术的领先地位。本文简要概括了细胞工厂和生物炼制这一新兴工业模式,回顾了生物炼制细胞工厂的重大计划和进展,讨论了目前亟待解决的关键问题和研究对策。     

Enzyme display technology for lignocellulosic biomass valorization by yeast cell factories

1. Download : Download high-res image (297KB)
2. Download : Download full-size image

     

Metabolic Engineering of Microbial Cell Factories for Biosynthesis of Flavonoids: A Review

Flavonoids belong to a class of plant secondary metabolites that have a polyphenol structure. Flavonoids show extensive biological activity, such as antioxidative, anti-inflammatory, anti-mutagenic, anti-cancer, and antibacterial properties, so they are widely used in the food, pharmaceutical, and nutraceutical industries. However, traditional sources of flavonoids are no longer sufficient to meet current demands. In recent years, with the clarification of the biosynthetic pathway of flavonoids and the development of synthetic biology, it has become possible to use synthetic metabolic engineering methods with microorganisms as hosts to produce flavonoids. This article mainly reviews the biosynthetic pathways of flavonoids and the development of microbial expression systems for the production of flavonoids in order to provide a useful reference for further research on synthetic metabolic engineering of flavonoids. Meanwhile, the application of co-culture systems in the biosynthesis of flavonoids is emphasized in this review.     

细胞工厂与生物炼制

生物炼制是以可再生生物质资源为原料,生产能源与化工产品的新兴工业模式.是转变经济增长模式,保障社会经济可持续发展的重大战略需求.微生物细胞工厂是生物炼制技术至关重要的核心.世界各国纷纷设立重大研究计划支持细胞工厂的研究,以期获得生物炼制技术的领先地位.本文简要概括了细胞工厂和生物炼制这一新兴工业模式,回顾了生物炼制细胞工厂的重大计划和进展,讨论了目前亟待解决的关键问题和研究对策.     

Current Advances in the Bacterial Toolbox for the Biotechnological Production of Monoterpene-Based Aroma Compounds

Monoterpenes are plant secondary metabolites, widely used in industrial processes as precursors of important aroma compounds, such as vanillin and (−)-menthol. However, the physicochemical properties of monoterpenes make difficult their conventional conversion into value-added aromas. Biocatalysis, either by using whole cells or enzymes, may overcome such drawbacks in terms of purity of the final product, ecological and economic constraints of the current catalysis processes or extraction from plant material. In particular, the ability of oxidative enzymes (e.g., oxygenases) to modify the monoterpene backbone, with high regio- and stereo-selectivity, is attractive for the production of “natural” aromas for the flavor and fragrances industries. We review the research efforts carried out in the molecular analysis of bacterial monoterpene catabolic pathways and biochemical characterization of the respective key oxidative enzymes, with particular focus on the most relevant precursors, β-pinene, limonene and β-myrcene. The presented overview of the current state of art demonstrates that the specialized enzymatic repertoires of monoterpene-catabolizing bacteria are expanding the toolbox towards the tailored and sustainable biotechnological production of values-added aroma compounds (e.g., isonovalal, α-terpineol, and carvone isomers) whose implementation must be supported by the current advances in systems biology and metabolic engineering approaches.     

基于DL/T645规约的嵌入式工业物联控制节点设计

随着互联网、大数据、物联网等技术的飞跃发展,以实现智能制造为目标的“互联网+工业”行动计划已拉开了大幕;为满足智慧工厂的发展需求,一种具有实时高效、安全准确的现场数据传输的物联控制节点成为了关键;文章针对传统制造工厂通信系统的传输速率低、网络建设复杂、可靠性差、维护困难等缺点;提出了一种基于DL/T645规约的电力线载波数据采集节点,以STM32微控制器、载波收发模块、节点工厂设备接口等硬件,以DL/T645通信规约为核心设计物联节点,保障工厂现场数据的实时稳定采集,为构建监控和管理一体化的智慧工厂提供实时性强、可靠性高的通信系统。