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Summary A rapid, simple and reproducible reversed-phase high-performance liquid chromatographic method was developed for the quantitative determination of curcumin, demethoxycurcumin and bis-demethoxycurcumin in ethanolic extracts of turmeric. The pigments were separated on a styrene-divinylbenzene copolymer column (Hamilton PRP-1), using an acetonitrile-water (5545, v/v) mobile phase. The pigments were monitored with a diode-array detector at 425 nm. The limit of detection was 10.2 ng curcumin, 11.1 ng demethoxycurcumin and 6.2 ng bis-demethoxycurcumin. Comparison of HPLC and spectrophotometric results for the determination of the total curcuminoid content for a number of turmeric samples, reveal that the spectrophotometric method invariably yielded higher results, indicating an overestimation of curcuminoids. 相似文献
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Isocratic reversed phase high performance liquid chromatographic (HPLC) method using RP C18 column was developed for simultaneous
determination of the curcuminoids. Mobile phase consisted of acetonitrile:0.1% trifluro-acetic acid (50:50) and flow rate
was 1.5 mL min−1 and elution was monitored at 420 nm. Validation in selected conditions showed that the chosen method is sensitive, selective,
precise and reproducible with linear response of detector for the simultaneous determination of curcumin (C), demethoxycurcumin
(DMC) and bis-demethoxycurcumin (BDMC). The limits of detection were 27.99, 31.91 and 21.81 ng mL−1 for C, DMC and BDMC, respectively. Limits of quantitation for C, DMC and BDMC, were 84.84, 96.72 and 66.10 ng mL−1, respectively. Linear range was form 100 to 600 ng mL−1. The mean ± SD percent recoveries of curcuminoids were 99.87 ± 0.34, 100.09 ± 0.48 and 100.10 ± 0.60% of C, DMC and BDMC,
respectively. Further, the method was used for quantitation of curcuminoids from turmeric rhizome. 相似文献
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Sreeraj Gopi Augustine Amalraj Shintu Jude K.T. Benson Preetha Balakrishnan Józef T. Haponiuk 《高分子科学杂志,A辑:纯化学与应用化学》2019,56(4):327-340
Turmeric spent, a by-product of turmeric processing industries, was used as a source to prepare nanofibers (NF). The chemical treatments methods followed by acid hydrolysis accompanied with high pressure homogenization were used to prepare NF. The resulting turmeric nanofibers (TNF) were characterized by Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray diffraction (XRD) and thermogravimetric analysis (TGA/DTA). The TNF presented needle like structure, high thermal stability, an average width of 38.5?nm, average length of 245.7?nm, and giving an aspect ratio (L/D) of 23.15. The prepared TNF showed pronounced antimicrobial activity against Bacillus cereus, Escherichia Coli, Salmonella typhimurium and Staphylococcus aureus and also registered good antioxidant activity. The results showed that TNF were successfully obtained from turmeric spent and might be potentially applied in different fields, such as pharmaceutical, biological active species, nutraceuticals, components for food industries and bionanocomposites. 相似文献
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Summary The conditions for thin-layer chromatographic determination of curcumine in turmeric root powder and spice-mixtures have been optimized. Separation of the methanolic extract on silica gel thin-layer plates with chloroform-acetic acid (8020) gave the best results. The curcumine can be directly determined on the layer by fluorometric scanning, after conversion to rubrocurcumine with a mixture of boric acid and oxalic acid as spray reagent. Determination of the amount of turmeric root powder in spice mixtures by determining the curcumine content is only possible if the curcumine content of the turmeric root powder used is known because different turmeric root powders may have different curcumine contents. 相似文献
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