Diffusion-limited energy transfer from Tb3+ → Nd3+ and Tb3+ → Ho3+ in DMSO

The energy transfer phenomenon has been studied from Tb³⁺ → Nd³⁺ and Tb³⁺ → Ho³⁺ in DMSO. A diffusion limited dipole-dipole mechanism of energy transfer is suggested for both systems. At high acceptor concentrations, P_da depends linearly on C² consistent with the Fong and Diestler theory of energy transfer. However, at low acceptor concentrations the observations of time evolutions of Tb³⁺ luminescence decay following flash excitation has enabled us to examine diffusion limited energy transfer from Tb³⁺ to Nd³⁺ and Tb³⁺ to Ho³⁺ in DMSO.     

DEVELOPMENT AND IDENTIFICATION OF CHINESE SPRING Ta1 kr ph1b PLANTS AND THEIR USE ON GENETIC TRANSFER OF DESIRABLE GENES FROM Aegilops

Chinese Spring Tal kr phlb plants were developed by hybridizing Tal common wheat and Chinese Spring phlb mutant and backcrosses. When Chinese Spring Tal kr phlb plants were crossed with some wild species of Triticeae, much more seeds of wide hybridization can be obtained without artificial emasculation and pollination because of the existence of Tat and kr genes. The homoeologous chromosomes between common wheat and the wild species of Triticeae can also pair and exhibit cross-over in the hybrid F1 plants because of the existence of the phlb gene. So that more alien desirable genes can be transferred to common wheat rapidly. Therefore, Chinese Spring Tal kr phlb plants become a powerful tool for wide hybridization and transferring alien desirable genes to common wheat.     

Application of a polyaniline based ammonium sensor for the amperometric immunoassay of a urease conjugated Tal 1 protein

An amperometric immunoassay was developed by coupling the enzyme-linked immunosorbent assay (ELISA) microtiter-plate system with a polyaniline-perfluorosulfonated ionomer composite (PA/NF) electrode incorporated flow injection analysis (FIA) system and used for the analysis of Tal 1 protein, found in leukemic T cell. Rabbit polyclonal antibody (pAb) against Tal 1 and urease-pAb were used, respectively as the captured protein and enzyme labeled conjugate for sandwich immunoassay of Tal 1. Characteristics of the PA/NF electrode such as reproducibility, stability and sensitivity were studied. The detection limits of the PA/NF electrode for NH₄⁺ and urease were found to be 5 μM and 0.05 nM, respectively. Assay conditions such as the amount of pAb needed for coating the plate, the concentration of urease-pAb conjugate appropriate for the immunoreaction and the incubation time for urea to react with the bound urease-pAb in the microtiter-wells were also studied. A detection limit as lower as 0.5 ng/ml and a dynamic range of 1.0-100 ng/ml were found for the immunoassay of Tal 1 protein with the developed immunoassay system.     

Emission spectra of CH(A 2Δ) from methane in an argon flowing afterglow

The CH A ²ΔX²Π emission was observed in the flowing afterglow reaction of charged species of argon with CH₄. Energetic considerations show that metastable argon ions, Ar^+M(⁴D, ⁴F, ²F, ²G, are plausible candidates, although the contribution of Ar²⁺ cannot be completely excluded. The CH A ²ΔX²Π spectrum was analyzed; the ratio of vibrational populations for the υ′ = 0 and 1 states, P_vib(υ′ = 0), is estimated to be 0.56 ± 0.17, and the effective rotational temperatures are (3.0 ± 1.5) × 10³ and (1.7 ± 0.4) × 10³ K, respectively.     

Sideritis romana L. subsp. purpurea (Tal. ex Benth.) Heywood,a new chemotype from Montenegro

A study on essential oil fractions of the Western Balkan endemic Sideritis romana L. subsp. purpurea (Tal. ex Benth.) Heywood collected in Montenegro is reported. The 24-h systematic steam distillation extraction procedure was performed. The gas chromatographic/mass spectrometric (GC/MS) analysis of the fractions showed γ-elemene and spathulenol as two main constituents, revealing a new chemotype of this plant species. Although varying in the content of these two main compounds, which makes the fractions quite different between each other, evaluation of the anti-Candida activity showed the lack of any significant efficacy.