新疆红景天红色素的提取及理化性质的研究

对用索氏提取法和乙醇浸取法提取红景天红色素进行了比较，并探讨浸取的浓度，浸取温度等因素，指出了最佳生产工艺条件，从红景天中提取的天红色素是一种安全无毒的食用色素，本文还研究了几种食品添加剂对红景天红色素稳定性的影响，并对色素的耐氧化性进行了探讨，旨在为红景天红色素的开发利用提供有实用价值的信息。     

天然和人工培植红景天挥发油的对比研究

应用ＧＣ－ＭＳ和ＧＣ－ＩＲ两种联机法对比研究了天然和人工培植红景天挥发油、分别鉴定出２７个和２４个化合物。ＭＳ给出结构信息和ＩＲ给出官能团信息相结合，提高了分析测试复杂样品的可靠性。     

Characterization of the chemical constituents in Hongjingtian injection by liquid chromatography quadrupole time‐of‐flight mass spectrometry

Hongjingtian injection is made from Rhodiola wallichiana and used in the treatment of stable angina pectoris associated with coronary heart disease. In this study, the chemical constituents in Hongjingtian injection were comprehensively studied using liquid chromatography quadrupole time‐of‐flight mass spectrometry. A total of 49 compounds were identified or assumed, including 10 organic acids, nine phenylethanoids, 10 phenylpropanoids, two flavonoid glycosides, seven monoterpene glycosides, seven octylglycosides and four other types of compounds. The structures of seven compounds were confirmed by comparing their retention times, MS and UV spectra with the corresponding authentic standards. Amongst the 49 compounds, 35 were firstly found in R. wallichiana, while they have been reported in other species of the genus Rhodiola, including Rhodiola crenulata, Rhodiola sacra, Rhodiola rosea and Rhodiola kirilowii. The possible fragmentation pathways in the mass spectrometry of the major types of compounds are proposed and summarized. Our study demonstrates a rapid method for characterizing the chemical constituents present in the Hongjingtian injection, which could also be applied to the identification of chemical constituents in other TCM formulae containing R. wallichiana.     

玫瑰红景天药用历史及功能简介

玫瑰红景天具有上千年的药用历史,是我国传统中药中的瑰宝。大量研究表明,玫瑰红景天提取物对人体中枢神经系统、心血管系统以及内分泌系统具有保护和调节作用,尤其在抗疲劳、抗抑郁、抗肿瘤及提高人体免疫力等方面具有独特的药效。本文介绍了玫瑰红景天的药用开发历史及其特征活性成分的功能,以期提高人们对这种传统中药的认识,为进一步开发玫瑰红景天的药用功能提供借鉴和参考。     

RP‐HPLC determination of phenylalkanoids and monoterpenoids in Rhodiola rosea and identification by LC‐ESI‐TOF

An HPLC method permitting the simultaneous determination of fourteen analytes (phenylalkanoids and monoterpenoids) from the roots of Rhodiola rosea was developed. A separation was achieved within 35 min using C₁₈ column material and a water–acetonitrile mobile phase, both containing a 0.05% phosphoric acid gradient system and a temperature of 53°C. The method was validated for linearity, repeatability, limits of detection and limits of quantification. The limits of detection and limits of quantification of 14 phenylalkanoids and monoterpenoids were found to be 0.20–1.0 and 0.5–3.5 µg/mL, respectively. The wavelengths used for quantification of phenylalkanoids and monoterpenoids with a diode array detector were 205, 220 and 251 nm. The method was used to analyze the roots of two species of Rhodiola and commercial extracts of R. rosea and provides preliminary evidence of phytochemical differences between North American and Eurasian populations of R. rosea. LC–mass spectrometry coupled with electrospray ionization (ESI) interface method is described for the identification of phenylalkanoids and monoterpenoids in various Rhodiola samples. This method involved the use of the [M + H]⁺, [M + NH₄]⁺ and [M + Na]⁺ ions in the positive ion mode with extractive ion monitoring (EIM). Copyright © 2009 John Wiley & Sons, Ltd.     

电感耦合等离子体-原子发射光谱法测定红景天中锌、铁、锰和钛

用电感耦合等离子体-原子发射光谱法测定红景天中锌、铁、锰和钛等元素。使用与分析样品基体相接近的标准样品和控制一致的测定条件,克服了物理干扰和基体效应的影响,选择波长为233.0,273.9,257.6,334.9nm4条谱线分别作为测定锌、铁、锰和钛的分析线,测得4种元素的检出限(3s)为0.004—0.05μg/mL。本法测定红景天中4种金属元素的含量,相对标准偏差(n=7)在2.2%—4.2%之间,加标回收率在96.4%—115.2%之间。     

一种高山红景天成分FT—IR和FT—Ramnm光谱的研究

本文对高山红景天提取物中的一种新化合物进行了红外和拉曼光谱的测定,并对主要谱带进行了归属和讨论。     

长白山地区几种红景天品种的傅里叶变换红外光谱法鉴别研究

分别用石油醚,氯仿,甲醇对长白山地区的野生和栽培高山红景天和长白红景天进行了提取,并首次对提取物进行了傅里叶变换红外光谱法测定,还用红景天块做了相应的验证研究。结果表明,长白山地区的野生和栽培高山红景天和长白红景天的红外光谱图谱有明显不同,故可用作鉴别其品种或评价其质量的一种手段。     

大花红景天多糖RCPS分离纯化及单糖组成分析

采用水提醇沉方法提取大花红景天粗多糖RCP(rhodiola crenulata polysaccharide),并通过乙醇分级沉淀,Sevag法脱除蛋白,葡聚糖凝胶柱色谱纯化等手段,得到一种多糖RCPS。采用苯酚-硫酸法测定了总糖含量,UV和IR等方法考察了多糖性质,凝胶渗透色谱-示差检测法测定多糖的纯度及分子量范围及分布,GC法鉴定单糖组成及其摩尔比值。结果表明,多糖RCPS为淡黄色粉末状物质,易溶于水,总糖含量为99.11%。紫外光谱分析显示,在195 nm 波长处有明显吸收峰,在260和280 nm等处无吸收峰,说明被测物为多糖,且不含核酸及蛋白质;红外吸收光谱分析表明,在3 424.83,2 934.10,1 742.11,1 438.96,1 261.40, 1 103.54,832.86 cm-1处均有明显的多糖特征吸收,主要由鼠李糖,阿拉伯糖,木糖,甘露糖,葡萄糖,半乳糖和半乳糖醛酸组成,其摩尔比值为1∶2.96∶0.21∶0.26∶0.08∶0.58∶0.15。     

The Effectiveness of Rhodiola rosea L. Preparations in Alleviating Various Aspects of Life-Stress Symptoms and Stress-Induced Conditions—Encouraging Clinical Evidence

Rhodiola rosea L. has a long history of use in traditional medicine to stimulate the nervous system, treat stress-induced fatigue and depression, enhance physical performance and work productivity and treat gastrointestinal ailments and impotence. Apart from its well-established traditional use, a significant number of publications on the clinical efficacy of various R. rosea preparations can be found in the literature. The majority of these studies are related to the efficacy of R. rosea in terms of cognitive functions and mental performance, including various symptoms of life-stress, fatigue and burnout. The beneficial effects of this medicinal plant on enhancing physical performance have also been evaluated in professional athletes and non-trained individuals. Moreover, even though most evidence originates from pre-clinical trials, several clinical studies have additionally demonstrated the remediating effects of R. rosea on cardiovascular and reproductive health by addressing non-specific stress damage and reversing or healing the disrupted physiologies and disfunctions. Overall, in accordance with its aim, the results presented in this review provide an encouraging basis for the clinical efficacy of R. rosea preparations in managing various aspects of stress-induced conditions.