全文获取类型
收费全文 | 181篇 |
免费 | 8篇 |
国内免费 | 10篇 |
专业分类
化学 | 98篇 |
晶体学 | 1篇 |
力学 | 2篇 |
数学 | 7篇 |
物理学 | 91篇 |
出版年
2024年 | 1篇 |
2023年 | 5篇 |
2022年 | 5篇 |
2021年 | 3篇 |
2020年 | 3篇 |
2019年 | 1篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 7篇 |
2014年 | 11篇 |
2013年 | 20篇 |
2012年 | 19篇 |
2011年 | 22篇 |
2010年 | 12篇 |
2009年 | 15篇 |
2008年 | 11篇 |
2007年 | 10篇 |
2006年 | 5篇 |
2005年 | 7篇 |
2004年 | 2篇 |
2003年 | 3篇 |
2002年 | 5篇 |
2001年 | 2篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1997年 | 1篇 |
1996年 | 4篇 |
1994年 | 1篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1985年 | 1篇 |
排序方式: 共有199条查询结果,搜索用时 15 毫秒
1.
稀土在鼠肝中的物种分布 总被引:23,自引:0,他引:23
采用 ICP-MS、微量蛋白层析分离技术 ,研究了长时间、低剂量混合稀土及短时间、高剂量硝酸镧分别对大鼠及小鼠作用后 ,稀土在鼠肝中的代谢累积及其物种分布 .结果表明 ,鼠肝中各稀土含量随给药剂量的增加而增大 ,其累积速度大小顺序为 La>Ce>Nd>Pr;经柱层析分离未得到与稀土特异结合的鼠肝蛋白 ;稀土可与多种鼠肝蛋白 (包括多种重要的酶 )结合 ,在一定条件下 ,稀土与其结合蛋白可发生解离 相似文献
2.
Development of a method for very low level selenium determination in water soluble protein and peptide fractions, obtained after various separation procedures, is presented. A hydride generation atomic fluorescence spectrometry (HG-AFS) detection system was optimised and the influence of Cu(II), Sb(V), As(III) and HNO3 interferences in the measurement of Se by HG-AFS was investigated. A destruction procedure using HNO3 and H2O2 was also optimised and the average recovery of the digestion of a solution of selenomethioneine was 92 ± 4% (n=14). Combination of this digestion with the detection system gave reliable results. Accuracy was tested by comparison with two independent methods. A very low detection limit (DL) of 0.2 ng/g of measuring solution was achieved. The whole procedure from weighing to measuring was performed in the same Teflon tube. The addition of HNO3 to the fractions before long term storage at -20°C was necessary to prevent adsorption on the test tubes.Selenium was measured in water soluble protein and peptide fractions obtained after extraction, and Sephadex G-75 chromatography performed on liver samples from: i) hens exposed to As2O3, ii) hens fed with a high fat feed and iii) the certified reference material dogfish liver (CRM DOLT-2). Because of the very low DL we were able to observe the Se distribution in chromatographic fractions of samples of organisms which were not exposed to excess amounts of Se. The presence of selenium associated with metallothioneins was observed. 相似文献
3.
Zwickl H Traxler E Staettner S Parzefall W Grasl-Kraupp B Karner J Schulte-Hermann R Gerner C 《Electrophoresis》2005,26(14):2779-2785
The secretome of cells and tissues may reflect a broad variety of pathological conditions and thus represents a rich source of biomarkers. The identity of secreted proteins, usually isolated from cell supernatants or body fluids, is hardly accessible by direct proteome analysis, because these proteins are often masked by high amounts of proteins actually not secreted by the investigated cells. Here, we present a novel method for the specific detection of proteins secreted by human tissue specimen as well as cultured cells and chose liver as a model. The method is based on the metabolic labelling of proteins synthesized during a limited incubation period. Then, the cell supernatant is filtered, precipitated, and subjected to two-dimensional gel electrophoresis. Whereas fluorography detected a large number of proteins derived from residual plasma and dead cells, the autoradiographs selectively displayed genuinely secreted proteins. We demonstrate the feasibility of this approach by means of the secretomes of the hepatocellular carcinoma-derived cell line HepG2 and human liver slices. The selective identification of cell- and tissue-specific protein secretion profiles may help to identify novel sets of biomarkers for wide clinical applications. 相似文献
4.
猪肝酯酸PLE(Pig Liver Esterase)对双酯具有特殊的不对称水解性,而且可获得高产率和高光学纯的不对称水解产物。本文简要介绍PLE作为一种有效的酶催化剂,在温和的条件下,对双酯的不对称水解反应和在不对称合成中应用的近况。 相似文献
5.
Socaciu C Jessel R Diehl HA 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2000,56(14):133-2809
The carotenoids beta-carotene (BC), lycopene (LYC), lutein (LUT), zeaxanthin (ZEA), canthaxanthin (CTX) and astaxanthin (ASTA) have been incorporated into pig liver microsomes. Effective incorporation concentrations in the range of about 1-6 nmol/mg microsomal protein were obtained. A stability test at room temperature revealed that after 3 h BC and LYC had decayed totally whereas, gradually, CTX (46%), LUT (21%), ASTA (17%) and ZEA (5%) decayed. Biophysical parameters of the microsomal membrane were changed hardly by the incorporation of carotenoids. A small rigidification may occur. Membrane anisotropy seems to offer only a small tolerance for incorporation of carotenoids and seems to limit the achievable incorporation concentrations of the carotenoids into microsomes. Microsomes instead of liposomes should be preferred as a membrane model to study mutual effects of carotenoids and membrane dynamics. 相似文献
6.
7.
《Arabian Journal of Chemistry》2020,13(2):4386-4403
Platinum Nanoparticles (PtNPs) are synthesized from the Anbara fruit (Phoenix dactylifera L.) and are characterized using various spectroscopic analytical methods. These PtNPs were used to study the Hepatotoxic and Hepatoprotective effects on acute liver damage caused by CCl4 in Wister rats. Seventy-two rats of both sexes are divided into twelve groups and are treated with PtNPs and aqueous Anbara extract (AAE). Histopathological examinations were performed to identify the toxic effects on the vital organs of the rats. Hepatoprotective activity was monitored by observing the serobiochemical and hematological parameters and the intensity of hepatic marker enzymes alanine transferase (ALT), aspartate amino transferase (AST) and alkaline phosphatase (ALP) in the organs such as liver, intestine and kidney. Considerable experimental results were obtained when compared with the standard drug Silymarine. The PtNPs and AAE were proven to have protective activity of enzymes in the liver of Wister rats. 相似文献
8.
9.
10.
Ralph Weissleder David D. Stark Guillermo Elizondo Peter F. Hahn Carolyn Compton Sanjay Saini Jack Wittenberg Joseph T. Ferrucci 《Magnetic resonance imaging》1988,6(6):675-681
Thirteen patients with biopsy proven hepatic lymphoma (2 Hodgkin, 11 Non-Hodgkin) and a control group of 15 patients with hepatic metastases were analyzed quantitatively and qualitatively by MRI. Focal hepatic lymphoma was most reliably detected (eight of eight patients) and appeared hypointense relative to liver on T1 weighted (CNR − 7.4 ± 2.3) and hyperintense on T2 weighted (CNR + 8.4 ± 2.9) images. The mean T1 and T2 relaxation times of focal hepatic lymphoma (T1 = 832 ± 234 msec, T2 = 84 ± 16 ms) differed significantly from adjacent non-tumorous liver (T1 = 420 ± 121 ms, T2 = 51 ± 9 ms; p < 0.05), however CNR values and relaxation times were similar to those of hepatic metastases. Diffuse hepatic lymphoma (microscopic periportal infiltration) was undetectable by MRI in three patients by either morphologic features or quantitative criteria. A mixed pattern of hepatic lymphoma (focal lesions and diffuse infiltration) showed focal areas of slightly decreased signal intensity on T1 weighted images (CNR = −1.7 ± 0.4) while T2 weighted images revealed multiple regions of focal hyperintensity (CNR = +13.3 ± 8.4) superimposed on a diffusely hyperintense liver. Our experience demonstrates that either T1 or T2 weighted techniques are useful in detecting focal and that T2 weighted techniques are useful in detecting mixed hepatic lymphoma. Conventional image derived relaxation time measurements and quantitative parameters were of no additional diagnostic value. 相似文献