良性与恶性多形性腺瘤组织结构的FTIR光谱研究

结合口腔医院临床需要,利用PE公司的Spectrum GX傅里叶变换红外光谱仪,通过对5例良性、4例恶性多形性腺瘤组织的FTIR光谱测试、计算与分析,进行了多形性腺瘤组织结构的FTIR光谱对比研究。测试结果表明： (1)同种性质组织的FTIR光谱表现出了良好的一致性,揭示了吸收峰体现了生物组织的特征红外谱;(2)良性与恶性瘤的FTIR吸收峰峰位及其强度有若干处不同,揭示了恶性肿瘤中蛋白质的酰胺Ⅱ带氢键化程度降低,而脂类的氢键化程度提高;(3)峰高比计算结果表明,良性多形性腺瘤相比,恶性瘤中核酸的含量相对于胶原蛋白的含量增加,脂类的含量相对于蛋白质的含量增加。     

Preparation of gelatin/Ag NPs under ultrasound condition: A potent and green bio-nanocomposite for the treatment of pleomorphic hepatocellular carcinoma,morris hepatoma,and novikoff hepatoma

Regarding applicative, facile, green chemical research, a bio-inspired approach is being reported for the sonochemical synthesis of Ag nanoparticles by gelatin as a natural reducing/stabilizing and solid support agent without using any toxic and harmful reagent. The biosynthesized Ag NPs@gelatin bionanocomposite were characterized by advanced physicochemical techniques like ultraviolet–visible (UV–Vis), Fourier Transformed Infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), High-Resolution Transmission Electron Microscopy (HR-TEM), Energy Dispersive X-ray spectroscopy (EDX), and X-ray Diffraction (XRD) study. It has been established that gelatin-stabilized silver nanoparticles have a spherical shape with a mean diameter from ～5–10 nm. To survey the antioxidant potentials of Ag NPs@gelatin, one of the common antioxidant techniques i.e., DPPH was used. The results of DPPH test proved excellent antioxidant properties of Ag NPs@gelatin in comparison to the positive control used i.e., butylated hydroxytoluene. The IC50 of Ag NPs@gelatin was 250 µg/mL in the antioxidant test. Determination of anti-liver cancer effects of Ag NPs@gelatin was carried out by the MTT assay and against pleomorphic hepatocellular carcinoma (SNU-387), morris hepatoma (McA-RH7777), and novikoff hepatoma (N1-S1 Fudr) cell lines. The results of MTT assay confirmed removing SNU-387, McA-RH7777, and N1-S1 Fudr cell lines after treating with low concentrations of Ag NPs@gelatin. The IC50 of the Ag NPs@gelatin was 192, 144, and 228 µg/mL against SNU-387, McA-RH7777, and N1-S1 Fudr cell lines, respectively. The best result of anti-human liver cancer effects of Ag NPs@gelatin against the above cell lines was seen in the case of the N1-S1 Fudr cell line. According to the above results, significant anti-liver cancer effects of Ag NPs@gelatin is confirmed.     

傅里叶红外光谱法用于体表检测涎腺多形性腺瘤的研究

应用中红外光纤法检测了20例涎腺多形性腺瘤患者腺体部位的体表皮肤,对得到的红外光谱进行分析比较,发现多形性腺瘤、多形性腺瘤癌变及正常腺体体表皮肤红外光谱的主要特征吸收峰存在差异,可以从各特征吸收峰的峰位、峰形及不同谱峰的强度比的变化来初步判定肿瘤的性质。根据红外光谱判断其中2例已经发生癌变,与病理结果一致。从红外光谱的特征来看,多形性腺瘤是正常涎腺组织向多形性腺瘤癌变转变的中间过程。