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Simultaneous quantitative determination of 20 active components in the traditional Chinese medicine formula Zhi‐Zi‐Da‐Huang decoction by liquid chromatography coupled with mass spectrometry: application to study the chemical composition variations in different combinations
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Zheng Tang Ran Yin Kaishun Bi Heyun Zhu Fei Han Kelin Chen Fenrong Wang 《Biomedical chromatography : BMC》2015,29(9):1406-1414
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Yongtao Bai Lihua Song Yongheng Zhang Guoliang Dai Weidong Zhang Shanshan Song Hong Sun Wen Jing Meijuan Xu Wenzheng Ju 《Biomedical chromatography : BMC》2019,33(9)
A highly selective and efficient LC–MS/MS method was developed to determine the plasma concentration of magnolol, hesperidin, neohesperidin and geniposide following oral administration of Zhi‐Zi‐Hou‐Po decoction in normal and depressed rats. Plasma samples were pretreated by protein precipitation with methanol. Chromatographic separation was performed on an XTerra® MS C18 column using a gradient elution with a mobile phase composed of acetonitrile–0.1% aqueous formic acid. The proposed method was validated to be specific, accurate and precise for the analytes determination in plasma samples. The calibration curves displayed good linearity over definite concentration ranges for the analytes. The intra‐ and inter‐day precision of the proposed method at three different levels were all within <11.13% and the relative errors ranged from ?8.46 to 8.93%. The recovery of the four compounds ranged from 82.72 to 89.08% and no apparent matrix effect was observed during sample analysis. After full validation, the established method was successfully applied for comparing the pharmacokinetics of four components between normal and depressed rats. The results showed that the AUC and Cmax of four analytes in depressed rats were significantly different from those in normal rats and might provide helpful information to guide the clinical use of Zhi‐Zi‐Hou‐Po to treat depression. 相似文献
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《Journal of separation science》2017,40(14):2941-2949
A novel hollow‐fiber liquid‐phase microextraction based on oil‐in‐salt was proposed and introduced for the simultaneous extraction and enrichment of the main active compounds of hesperidin, honokiol, shikonin, magnolol, emodin, and β,β′‐dimethylacrylshikonin in a formula of Zi‐Cao‐Cheng‐Qi decoction and the single herb, Fructus Aurantii Immaturus , Cortex Magnoliae Officinalis , Radix et Rhizoma , and Lithospermum erythrorhizon , composing the formula prior to their analysis by high‐performance liquid chromatography. The results obtained by the proposed procedure were compared with those obtained by conventional hollow‐fiber liquid‐phase microextraction, and the proposed procedure mechanism was described. In the procedure, a hollow‐fiber segment was first immersed in organic solvent to fill the solvent in the fiber lumen and wall pore, and then the fiber was again immersed into sodium chloride solution to cover a thin salt membrane on the fiber wall pore filling organic solvent. Under the optimum conditions, the enrichment factors of the analytes were 0.6–109.4, linearities were 0.002–12 μg/mL with r 2 ≥ 0.9950, detection limits were 0.6–12 ng/mL, respectively. The results showed that oil‐in‐salt hollow‐fiber liquid‐phase microextraction is a simple and effective sample pretreatment procedure and suitable for the simultaneous extraction and concentration of trace‐level active compounds in traditional Chinese medicine. 相似文献
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UV/Vis光谱法研究红子色素的稳定性 总被引:1,自引:0,他引:1
本文报导了从红子提取,分离天然色素及酸碱和温度等对该色素稳定性的影响。 相似文献
6.
Identification of chemical constituents in traditional Chinese medicine formula using HPLC coupled with linear ion trap‐Orbitrap MS from high doses of medicinal materials to equivalent doses of formula: Study on Xiang‐Sha‐Liu‐Jun‐Zi‐Jia‐Jian granules
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Zhiwei Lu Qing Wang Meiling Wang Yuehong Liu Shuang Fu Xiaoyan Gao Xudong Tang 《Journal of separation science》2016,39(9):1619-1627
High‐resolution mass spectrometry has been a powerful tool for the research of chemical constituents in traditional Chinese medicine (TCM) formulas. However, the chromatographic peaks were difficult to discriminate clearly in data collection or analysis because of the complexity and the greatly different content of the constituents in TCM formula, which increased the difficulty of identification. In this study, a high‐performance liquid chromatography coupled with linear ion trap‐Orbitrap mass spectrometry based strategy focused on the comprehensive identification of TCM formula constituents was developed. Identification was carried out from a high dose of medicinal materials to equivalent dose of formula. Meanwhile, combined with mass spectrometry data, chromatographic behaviors, reference standards and previous reports, the identification of constituents in Xiang‐Sha‐Liu‐Jun‐Zi‐Jia‐Jian granules was described. 169 compounds were unambiguously or tentatively characterized, mainly including flavonoids, alkaloids, triterpenic acids, triterpene saponins, lactones, sesquiterpenoids and some other compounds. Among them, 11 compounds were unambiguously confirmed by comparing with reference standards. These results demonstrated that the method was effective and reliable for comprehensive identification of constituents of Xiang‐Sha‐Liu‐Jun‐Zi‐Jia‐Jian granules extracts and reveal the material basis of its therapeutic effects. This strategy might propose a research idea for the characterization of multi‐constituents in TCM formula. 相似文献
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Identification of the constituents and metabolites in rats after oral administration of Zi Shen Formula by UPLC‐Q‐TOF/MS combined pattern recognition analysis
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《Biomedical chromatography : BMC》2018,32(2)
An ultra‐high‐performance liquid chromatography mass spectrometry method was established to detect and identify the chemical constituents of Zi Shen Formula (ZSF) and its metabolites in serum, urine and feces, after oral administration to rats. A total of 68 compounds were characterized in ZSF extracts. In vivo, 38 prototype components and 32 metabolites of ZSF were tentatively identified in rat serum, urine and feces. Seven metabolic pathways including demethylation, hydroxylation, oxidation, sulfation, glucuronidation, methylation and de‐caffeoyl were proposed to be involved in the generation of these metabolites. It was found that glucuronidation, methylation and demethylation were the major metabolic processes of alkaloids, while demethylation, methylation, sulfation and de‐caffeoyl were the major metabolic pathways of phenylethanoid glycosides. The main metabolic pathways of steroidal saponins were oxidation and isotype reactions. These findings are significant for our understanding of the metabolism of ZSF. The proposed metabolic pathways of bioactive components might be crucial for further studies of the mechanisms of action and pharmacokinetic evaluations of ZSF. 相似文献
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东晋道士发明火药新说 总被引:1,自引:0,他引:1
唐代道士最先发明火药的说法似乎已为学界所普遍接受.但是通过对东晋道教炼丹经诀<太上八景四蕊紫浆五珠降生神丹方经>的研究,发现还在东晋时,道士炼丹配方中所用的雄黄、雌黄、硝石和薰陆香等粉末状药物,事实上就已经包含了传统火药中"一硝、二黄、三木炭"的基本组成成份,而且为了防止爆炸的发生,道士们还有意采取了多种防范措施.这表明最初的火药雏形似可上溯至东晋甚至更早,从而有可能改写唐代火药发明说这一影响巨大的结论,并将火药的发明时间至少提前约550年. 相似文献
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雷震子护康胶囊制剂抗氧化和对DNA损伤保护作用研究 总被引:3,自引:0,他引:3
雷震子护康胶囊以低聚原花青素、灵芝多糖、绞股蓝总皂甙及一定量的维生素C等为主要成分而制成,有多种保健功能。为研究该复方制剂的抗氧化作用,采用七种化学发光体系和一种比色体系研究了雷震子直接清除活性氧、活性氮和对^.OH引起的DNA损伤的保护作用。结果表明雷震子能有效清除O2^-.、^.OH、H2O2、ONOO^-和全血嗜中性白细胞“呼吸爆发”产生的活性氧,抑制脂质过氧化,保护DNA免受^.OH的氧化损伤。这些结果提示,雷震子是一种有效的抗氧化复方制剂,其强烈的抗氧化活性可能是其保健功能的重要药理基础之一。 相似文献
10.
Rapid discovery of absorbed constituents and metabolites in rat plasma after the oral administration of Zi Shen Wan using high‐throughput UHPLC–MS with a multivariate analysis approach
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Xian‐Na Li Aihua Zhang Hui Sun Yanhua Song Di Zou Xijun Wang 《Journal of separation science》2016,39(24):4700-4711
Zi Shen Wan is a typical formula consisting of three herbs, Phellodendri Amurensis Cortex, Rhizoma Anemarrhenae, and Cortex Cinnamomi, and has been widely used for treating prostatitis and infection diseases. However, it lacks in‐depth research of the constituents of Zi Shen Wan in vivo and in vitro. In this work, ultra high performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry and MassLynx software was established to characterize the chemical compositions of Zi Shen Wan in vivo and in vitro. In total, 92 peaks were characterized in vitro and 33 peaks were characterized in vivo based on mass spectrometry and tandem mass spectrometry data. Among the 33 compounds characterized in rat plasma, 22 prototype components absorbed in rat serum and 11 metabolites were identified in vivo. This work was fully reports the chemical constituents of traditional Chinese formula of Zi Shen Wan, it demonstrated that ultra high performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry coupled to MassLynx software and multivariate data processing approach could be successfully applied for rapid screening and comprehensive analysis of chemical constituents in vitro and prototype components or metabolites in vivo of traditional Chinese medicine. 相似文献