An effective and economical method for the storage of plasma samples using a novel freeze-drying device

Biological samples, especially plasma samples, are conventionally stored under freezing conditions to maintain sample integrity prior to the detections of analytes. However, the storage of samples in a low-temperature environment is electric energy consuming, and the preparation of samples prior to analytes detection may be complicated. In this work, an effective and economical method was proposed to freeze-dry the samples using a novel device to allow subsequent storage of samples at ambient temperature. The sample preparations integrated in the new method are simple and easy to follow. Analytes were directly extracted with the extraction agent before sample injections. This new method was validated with quality control (QC) samples of levetiracetam and mycophenolic acid (MPA), and it was also applied to the pharmacokinetic (PK) studies of both drugs in healthy volunteers. When QC samples were stored and prepared with the new method, the detections of analytes were accurate and repeatable, and the analytes maintained stability for a long time. The PK studies of levetiracetam and MPA in healthy volunteers showed that the PK parameters of analytes stored with the new method were consistent with those stored with the conventional method. In conclusion, this effective and economical method is a practical option in reality and can play a big role in clinical and scientific drug researches.     

在不同条件下冻干猪主动脉的过程及结果的分析

本实验将猪主动脉血管的冷冻干燥与微CT扫描相结合,对不同一次干燥和二次干燥温度下的猪主动脉血管进行非侵入的扫描.通过计算样品的失水率,比较出快冻与慢冻对样品的影响,及不同一次干燥和二次干燥温度下对冻干的猪主动脉血管造成的差异,并比较这些差异,得出冻干的最优条件.同时,也对不同冻干条件下样品表面折皱的照片和CT扫描的图像...