全文获取类型
收费全文 | 1379篇 |
免费 | 34篇 |
国内免费 | 166篇 |
专业分类
化学 | 1265篇 |
力学 | 6篇 |
综合类 | 1篇 |
数学 | 4篇 |
物理学 | 303篇 |
出版年
2024年 | 1篇 |
2023年 | 51篇 |
2022年 | 36篇 |
2021年 | 55篇 |
2020年 | 45篇 |
2019年 | 89篇 |
2018年 | 76篇 |
2017年 | 113篇 |
2016年 | 68篇 |
2015年 | 47篇 |
2014年 | 86篇 |
2013年 | 70篇 |
2012年 | 92篇 |
2011年 | 90篇 |
2010年 | 60篇 |
2009年 | 79篇 |
2008年 | 86篇 |
2007年 | 74篇 |
2006年 | 75篇 |
2005年 | 45篇 |
2004年 | 55篇 |
2003年 | 33篇 |
2002年 | 20篇 |
2001年 | 19篇 |
2000年 | 14篇 |
1999年 | 13篇 |
1998年 | 7篇 |
1997年 | 7篇 |
1996年 | 5篇 |
1995年 | 9篇 |
1994年 | 10篇 |
1993年 | 9篇 |
1992年 | 9篇 |
1991年 | 5篇 |
1990年 | 16篇 |
1989年 | 1篇 |
1988年 | 3篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 1篇 |
排序方式: 共有1579条查询结果,搜索用时 605 毫秒
1.
A nanomaterials-based novel molecular beacon has attracted growing attentions in fluorescent assays as many nanomaterials possess excellent quenching efficiency. In this work, a gold-based nanobeacon probe was established to detect organophosphorus pesticides for the first time. The constructed gold-based nanobeacon acted as a signal indicator and could display the decreasing of the intensity in the presence of targets, which competitively bound to single strand DNA. To achieve a high sensitive probe, some parameters including solution pH, temperature and reaction time were investigated and optimized. The gold-based nanobeacon probe assay was proved to be rapid and sensitive to achieve a detection limit of 0.035 μM for isocarbophos, 0.134 μM for profenofos, 0.384 μM for phorate and 2.35 μM for omethoate, respectively. The prepared nanobeacon effectively reduced the background and improved the detection sensitivity and selectivity. The probe is stable, easy to operate and does not need sophisticated instruments. These features makes the probe feasible for screening trace organophosphorus pesticides in real samples. 相似文献
2.
设计合成了用以检测过渡金属离子的荧光化学敏感器体系,它们是由1,8-萘二酰亚胺为荧光团,多胺衍生物为金属离子受体组成.在室温下对其光物理性质的研究中发现,在没有加入过渡金属离子时,由于体系内存在有效的光诱导电子转移过程使得荧光团的荧光被淬灭.加入过渡金属离子后,金属离子受体中的氮原子和过渡金属离子之间的配位作用阻断了光诱导电子转移过程,体系的荧光增强.不同的金属离子受体表现出了和过渡金属离子不同的配位识别能力,并且通过荧光的变化传递出受体-金属离子作用的信息. 相似文献
3.
4.
5.
6.
Ana M. Piloto 《Tetrahedron》2006,62(39):9258-9267
Four carboxylic fused furans are presented as new fluorescent labels for the amino and hydroxyl functions of organic molecules. Various representative l-amino acids were chosen as models, labelled at their N-terminus and also at their side-chain. Fluorescent derivatives were obtained in high yields, and their absorption and emission properties were studied. 相似文献
7.
平面物体在曲面状态下扫描仪图像的校正理论 总被引:6,自引:5,他引:1
平面物体在曲面状态下经扫描仪扫描后,其图像将发生复杂的畸变。提出将其分类为灰度畸变、投影畸变和成像畸变。通过理论分析,提出了在二元曲面模型下对投影畸变和成像畸变进行数字校正的方法,给出了对灰度畸变进行数字校正的实用方法。 相似文献
8.
9.
As a kind of special functional microspheres, fluorescent polymer microspheres could be used in cell label and separation, blood flow assay, flow cytometer marking, chemical reaction assay,and in analyst of the transform and diffusion of particles in soil 1. However, one of the most important applications of fluorescent microspheres is in the high-throughput screening of drugs (HTS) 2. Through affinity interaction, radioactive ligands (latent drugs) are bound to fluorescent microspheres covered by receptor, and luminescence is produced by radioactivity, so ligands can be assayed and screened.In this study, we developed a technique for preparing micron-size fluorescent microspheres with different functional groups. The methods included the synthesis of micron-size polystyrene microspheres through the dispersion polymerization of styrene in different media such as ethanol,ethanol-water, and isopropanol; the functional polystyrene microspheres were prepared by introduction of functional monomers into the reaction system of styrene; the functional fluorescent microspheres were obtained by the way of dying functional microspheres in the fluorescent material's ethanol solvent.The average diameter of microspheres was in the range of 1~10 μm, and the distribution was normal distribution. The functional groups included -OH, -CHO, -COOH, -CONH2, and SO3H. The absorbing spectrum and exciting spectrum were tested, the results showed that the maximal absorbance of fluorescent microsphere was near 306.5 nm, and its maximal excitation was near 362 nm. The excitation spectrum of fluorescent material (DPO) and fluorescent microspheres were shown in figure 1, and it indicated that the developed fluorescent microspheres showed the same excitation behavior like DPO, which related to the fluorescent microspheres had stable luminescence property. 相似文献
10.
荧光钙探针试剂及其在生物分析中的应用 总被引:2,自引:0,他引:2
本文评论了荧光钙探针试剂合成、性质、结构和生物分析应用方面的进展。各种典型的荧光钙探针试剂均已分别加以讨论。 相似文献