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Beatrice Malgesini Eduard Felder Nicola Mongelli Gianluca Papeo 《Molecular diversity》2009,13(1):53-56
Polyfluorinated N-α-Fmoc--Boc-l-lysine represents the best-in-class among a set of polyfluorinated amino acids (PFAs) which are useful tools for 19F NMR-Based Screening. In this communication, optimized reaction conditions that allowed for the multi-gram preparation of
this unnatural amino acid are reported. 相似文献
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The technical and practical aspects of 19F NMR‐based screening against a macromolecular target are analyzed in detail. A novel method utilizing the relaxation of 19F homonuclear double quantum coherence is proposed for performing NMR‐based binding assays in a direct‐ or competition‐mode format. A combined strategy based on 19F NMR chemical shift prediction, 2D 19F NMR DOSY, and 2D 19F–1H NMR long‐range COSY experiments is presented for the deconvolution of complex mixtures of fluorinated molecules generated by either addition of single compounds or by chemical synthesis. The approaches presented here allow the screening of complex mixtures, even in the case where the exact composition is not known, and the rapid identification of the binders contained in the mixtures. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
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