Synthesis of orthogonal end functionalized oligoethylene glycols of defined lengths

The synthesis of oligoethylene glycols of defined lengths possessing different end functionalities is described. The utility of these molecules towards the development of a generic membrane anchor is demonstrated.     

基于联合分步APIT定位算法的改进

本文研究了无线传感网络( Wireless Sensor Network,WSNs)的节点定位问题,并针对APIT由于锚节点在低密度环境下的节点误判和节点失效等问题给出了改进,在APICT定位算法的基础提出了联合分步定位算法UNION-APICT(Union Approximate Point-In-Circumcircle Test),该算法是结合连通性的测距技术,RSSI测距技术以及质心定位和APICT等技术,来联合解决对未知节点定位问题。通过仿真实验结果表明,改进后的UNION-APICT在APICT算法的基础之上平均定位误差减少了10%-25%,定位性能有了明显的提升;随着通信半径R和最大探测距离rmax的增加,定位误差也在逐渐减小,该算法较APIT和APICT定位算法在锚节点密度、节点覆盖率和定位精度上都有所提高。     

Semisynthesis of Functional Glycosylphosphatidylinositol‐Anchored Proteins

Glypiation is a common posttranslational modification of eukaryotic proteins involving the attachment of a glycosylphosphatidylinositol (GPI) glycolipid. GPIs contain a conserved phosphoglycan that is modified in a cell‐ and tissue‐specific manner. GPI complexity suggests roles in biological processes and effects on the attached protein, but the difficulties to get homogeneous material have hindered studies. We disclose a one‐pot intein‐mediated ligation (OPL) to obtain GPI‐anchored proteins. The strategy enables the glypiation of folded and denatured proteins with a natural linkage to the glycolipid. Using the strategy, glypiated eGFP, Thy1, and the Plasmodium berghei protein MSP1₁₉ were prepared. Glypiation did not alter the structure of eGFP and MSP1₁₉ proteins in solution, but it induced a strong pro‐inflammatory response in vitro. The strategy provides access to glypiated proteins to elucidate the activity of this modification and for use as vaccine candidates against parasitic infections.     

输电铁塔四地脚螺栓塔脚板抗拉承载力试验与计算方法研究

针对架空输电线路铁塔中常用的四地脚螺栓塔脚板进行了抗拉承载力试验和理论计算方法研究。首先,选取8个试件进行塔脚板抗拉承载力试验研究,分析塔脚底板板厚及有无加劲板对塔脚板抗拉承载力的影响;其次,通过有限元模拟塔脚板的应力分布情况,并结合“塑性分析,弹性设计”的思想,提出了一种基于屈服线理论的四地脚螺栓塔脚板抗拉承载力计算公式;最后,与试验结果、有限元仿真结果、已有技术规定中的计算结果进行了对比。结果表明:建议公式与试验结果和仿真结果较为吻合,相关技术规定中的计算结果偏于保守,验证了本文建议公式的精确性;底板厚度和加劲板的作用对塔脚板承载力影响较大。研究结果可为输电铁塔四地脚螺栓塔脚板结构设计提供参考。     

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利用模拟装置实现了卡瓦与套管在地面上的实验，使用电测的方法测量胀卡时套管外表面的应变．由实验数据获得计算网格节点处的应变，同时利用单位咬合力作用套管的有限元结果进行数值法计算，获得卡瓦各咬合齿对套管咬合力的分布，为卡瓦的优化设计提供了可靠数据．     

Comparison of monomethoxy‐, dimethoxy‐, and trimethoxysilane anchor groups for surface‐initiated RAFT polymerization from silica surfaces

The immobilization of reversible addition–fragmentation chain transfer (RAFT) agents on silica for surface‐initiated RAFT polymerizations (SI‐RAFT) via the Z‐group approach was studied systematically in dependence of the functionality of the RAFT‐agent anchor group. Monoalkoxy‐, dialkoxy‐, and trialkoxy silyl ether groups were incorporated into trithiocarbonate‐type RAFT agents and bound to planar silica surfaces as well as to silica nanoparticles. The immobilization efficiency and the structure of the bound RAFT‐agent film varied strongly in dependence of the used solvent (toluene vs. 1,2‐dimethoxyethane) and the anchor group functionality, as evidenced by atomic force microscopy, transmission electron microscopy, dynamic light scattering, and UV/Vis spectroscopy. Surface‐initiated RAFT polymerizations using functionalized silica nanoparticles revealed that grafted oligomers, which often occur in SI‐RAFT, are not formed within the crosslinked structures that originate from the immobilization, and that RAFT‐agent films that show less aggregation during the immobilization are more efficient during SI‐RAFT in terms of polymer grafting density. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2015 , 53, 103–113     

单链系泊浮体受外碰撞时的锚链动态张力

介绍系泊浮体受外碰撞时锚链动态张力计算方法,并对动态张力的若干问题进行了讨论．     

Examining ligand-protein interactions with binding-energy landscapes

Binding-energy landscapes are used to investigate the thermodynamics of molecular recognition for the pteridine ring, a recognition anchor in binding with dihydrofolate reductase, and two molecules with the same shape but different heteroatom substitutions. The relative importance of hydrogen bonding and hydrophobic interactions in this system is analyzed by comparing these three different decorations of the pteridine scaffold. Received: 5 May 1998 / Accepted: 3 September 1998 / Published online: 17 December 1998     

偶应力理论下拉力型锚固体的受力特性

锚固体的受力特征及其影响因素是锚固体设计的重要依据,直接影响锚固效果。传统的经典弹性理论没有考虑应变梯度的影响。偶应力理论引进弯曲曲率,考虑了弯曲效应对介质变形特性的影响。基于偶应力理论,建立了平面应变问题的有限元计算模型,研究锚固体锚固段界面上的剪应力分布、锚固体轴力分布、偶应力的尺度效应以及弹性模量和围压对锚固力的影响,并将偶应力理论的计算结果和经典弹性理论的计算结果进行了比较。结果表明,在偶应力理论下,锚固体锚固段界面的剪应力有所减小,特别是峰值处的剪应力减小明显;岩土的弹性模量越大,锚固界面局部剪应力越大;锚固力随着围压的增大而增大,偶应力尺度效应明显。     

基于噪声误差模型的不规则区域室内定位和锚点分布优化

结合实际工业背景,研究了一类在不规则区域且误差不服从高斯分布的室内无线定位问题.给出了噪声误差模型, 在对多个传统定位算法进行性能分析的基础上,研究了待定位区域内锚点阵列的分布, 改进了多锚点阵列下的定位方法,并提出基于Delaunay三角剖分锚点分布优化模型和求解方法.