Disjointly homogeneous Banach lattices: Duality and complementation

We study several properties of disjointly homogeneous Banach lattices with a special focus on two questions: the self-duality of this class and the existence of disjoint sequences spanning complemented subspaces. Various results around these problems are given. In particular, we provide examples of reflexive disjointly homogeneous spaces whose dual spaces are not disjointly homogeneous.     

RNA detection using peptide-inserted <Emphasis Type="Italic">Renilla</Emphasis> luciferase

A novel complementation system with short peptide-inserted-Renilla luciferase (PI-Rluc) and split-RNA probes was constructed for noninvasive RNA detection. The RNA binding peptides HIV-1 Rev and BIV Tat were used as inserted peptides. They display induced fit conformational changes upon binding to specific RNAs and trigger complementation or discomplementation of Rluc. Split-RNA probes were designed to reform the peptide binding site upon hybridization with arbitrarily selected target RNA. This set of recombinant protein and split-RNA probes enabled a high degree of sensitivity in RNA detection. In this study, we show that the Rluc system is comparable to Fluc, but that its detection limit for arbitrarily selected RNA (at least 100 pM) exceeds that of Fluc by approximately two orders of magnitude.