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子宫内低铅暴露对婴儿早期神经行为影响的研究 总被引:2,自引:0,他引:2
为探讨低铅暴露对婴儿智能发育的影响,随机选择足月顺产新生儿110例,0.48 μmol/L>脐血铅水平≥0.24 μmol/L,作为观察组;同一时期配对足月顺产新生儿110例,脐血铅水平<0.24 μmol/L,作为对照组;采用新生儿20项新生儿神经行为、Gesell婴幼儿发育检查量表,在出生24~36 h和3月龄时进行测试.结果表明,观察组神经行为总得分显著性低于对照组;头竖立得分显著低于对照组,头竖立时间较对照组缩短;3月龄观察组适应性、语言能力和个人社会交往显著低于对照组. 提示血铅水平低于0.48 μmol/L时,仍可对婴儿神经行为发育产生不良影响. 相似文献
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高效液相色谱-质谱联用测定婴幼儿配方奶粉中的左旋肉碱 总被引:2,自引:0,他引:2
建立了婴幼儿配方奶粉中左旋肉碱含量的高效液相色谱-质谱联用测定方法。采用C4色谱柱(4.6mm×250 mm,5μm)进行分离,以甲醇-水(10∶90)为流动相,柱温25℃,流速1.0 mL.min-1,进样量为10μL,柱后分流比为1∶3,质谱采用多离子反应监测(MRM)方式进行检测,正离子模式,定量离子为m/z 162.2→103.1。在优化条件下,样品在10 min内完成分析,左旋肉碱在0.40~10.0 mg.L-1范围内线性关系良好(r=0.999 3)。方法的加标回收率为87%~106%,检出限(S/N=3)和定量下限(S/N=10)分别为39.0、116.9 ng。该方法操作简便、灵敏度高、重复性好,可用于婴幼儿配方奶粉中左旋肉碱的测定。 相似文献
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婴幼儿低发锌、铜、铁、钙与疾病的关系探讨 总被引:6,自引:1,他引:6
对846例低发锌、456例低发铜、488例低发铁以及595例低发钙婴幼儿与疾病的关系进行了探讨。结果显示,低发锌婴幼儿中反复呼吸道感染、食欲不振和异食癖的患病率分别为71.16%、57.57%和11.47%;低发铜和低发铁婴儿的贫血率分别为15.79%和73.16%,呼吸道感染的患病率分别为9.43%和35.66%:低发钙者中佝楼病的发病率为81.51%。 相似文献
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该文建立了婴幼儿配方乳粉中5种游离核苷酸的检测方法。婴幼儿配方乳粉经乙二胺四乙酸二钠和氯化钠溶液提取,强阴离子(SAX)固相萃取柱净化,通过反相Atlantis T3色谱柱分离,二极管阵列检测器检测,外标法定量。结果表明:方法抗干扰能力强、准确度高,尤其对羊奶粉净化效果好、分离度高。5种游离核苷酸在0.5~10 mg/L范围内线性关系良好(r≥0.999);添加水平在0.05~0.50 g/kg时,回收率为91.1%~112%,相对标准偏差为2.3%~4.7%;检出限为0.0010~0.0015 g/kg,定量限为0.0030~0.0045 g/kg。对乳粉质控样品进行检测,结果与中位值比较的偏差在10%以内。该方法可为监管部门提供技术支持,为乳品行业健康发展提供保障。 相似文献
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基于高分辨质谱技术的婴幼儿食品中过敏原蛋白质的高灵敏检测 总被引:2,自引:0,他引:2
基于高效液相色谱-串联质谱(LC-MS/MS)技术,选择稳定性好、灵敏度高的特征肽段,利用平行反应监测(PRM)技术,实现了多类过敏原蛋白质的高灵敏度同时检测,并成功应用于婴幼儿食品中过敏原成分的分析。对于婴幼儿食品中蛋白质的提取,与传统的丙酮沉淀法比,采用膜上原位样品预处理方法(i-FASP)可实现更高的蛋白质提取效率和抗干扰能力。所检测的过敏原蛋白质的定量限(LOQ)最小可达到0.028 mg/L,其线性范围最宽可跨越4个数量级,且线性关系良好(相关系数R~2≥0.99)。该方法为食品中过敏原蛋白质组学快速分析提供了一种可靠的分析方法。 相似文献
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Neeta Devi Sharma Ishwar D Sharma Rajeshwar Singh Chandel John C Wise 《International journal of environmental analytical chemistry》2016,96(3):225-236
This study documents the levels of pesticide residues in milk samples of mothers from Himachal Pradesh, India, and time trend comparison of pesticide load based on various studies conducted around the world. The regional difference in xenobiotic levels of breast milk varied with demographic characteristics of mothers and altitudinal variations. The single or multiple pesticides contamination of p,p′-DDE, p,p′-DDT and chlorpyrifos was revealed in 27.45% mothers’ milk samples. Among these p,p′-DDE was the major contaminant found in 26.79% samples followed by p,p′-DDT (1.31%) and chlorpyrifos (0.65%). However, residues of other 26 pesticides comprising organochlorines, organophosphorus and synthetic pyrethroids included in this study were below detectable limit (BDL). The determination of a low DDT/DDE ratio (0.050) indicated past exposure of mothers to DDT from the environment. The pesticide residues level in samples drawn from 14 branded infant formulae was BDL. The calculated infants’ daily intake (DI) of DDT was 0.0015 mg kg?1 body weight per day compared with a decade-old study (0.021 mg kg?1 body weight per day) suggesting a sharp decline in the residue levels of these pesticides in the Himalayan region. The trend comparison with past studies conducted around the world indicate a decline in the levels of organochlorine pesticide residues in mothers’ milk and further drop of DI in infants. However, such comparisons confer very limited utilisation of data generated on pesticide load in mothers’ milk and simultaneous infants’ DI due to lack of proper research protocol. 相似文献
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建立了一种同时测定婴幼儿配方乳粉中4种可选择成分(胆碱、左旋肉碱、牛磺酸和肌醇)的高效液相色谱-串联三重四极杆质谱(HPLC-MS/MS)分析方法。样品经温水溶解后用亚铁氰化钾和乙酸锌沉淀蛋白,上清液过滤后采用HSS T3色谱柱分离,三重四极杆质谱仪检测,胆碱和左旋肉碱使用内标法定量,牛磺酸和肌醇使用外标法定量。在最优化条件下,胆碱和左旋肉碱在0.01~2.0 mg/L范围内,牛磺酸和肌醇在0.1~2.0 mg/L范围内呈良好的线性关系,相关系数均大于0.997;胆碱和左旋肉碱的检出限均为1.5 mg/kg,牛磺酸和肌醇的检出限均为15 mg/kg。4种化合物的回收率为87.5%~102.4%,相对标准偏差(RSD,n=6)为3.0%~7.3%。该方法灵敏度高、净化效果好、定量准确,适用于婴幼儿配方乳粉中胆碱、左旋肉碱、牛磺酸和肌醇的同时快速检测。 相似文献
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儿童哮喘与血铅水平关系的研究 总被引:1,自引:0,他引:1
目的 :为探讨儿童铅中毒与哮喘的相互关系。方法 :采用电感耦合高频等离子体原子发射光谱法对 66例哮喘患儿及 60例健康儿童进行了血铅含量检测。结果 :( 1 )哮喘患儿血铅水平明显高于正常对照组 ;( 2 )血铅水平与哮喘严重度为正相关 ,r=0 0 93 ,P <0 0 5 ;( 3 )哮喘组与对照组儿童血铅水平t检验 ,有显著性差异 ,t=2 1 ,P <0 0 5。结论 :儿童哮喘存在着血铅水平升高 ,两者可能互为因果关系 相似文献
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Aurora Martín-Calero 《Talanta》2009,79(3):590-597
The beneficial effects of several ionic liquids (ILs) as mobile phase additives in high-performance liquid chromatography with electrochemical detection for the determination of six heterocyclic aromatic amines (HAs) have been evaluated for first-time. The studied ionic liquids were 1-butyl-3-methylimidazolium tetrafluoroborate (BMIm-BF4), 1-hexyl-3-methylimidazolium tetrafluoroborate (HMIm-BF4) and 1-methyl-3-octylimidazolium tetrafluoroborate (MOIm-BF4). Several chromatographic parameters have been evaluated in the presence or absence of ILs, or using ammonium acetate as the most common mobile phase additive, with three different C18 stationary phases. The effect of the acetonitrile content was also addressed. In general, best resolution, lower peak-widths (up to 72.1% lower) and lower retention factors are obtained when using ILs rather than ammonium acetate as mobile phase additives. The main improvement was obtained in the baseline noise, being 360% less noisy for BMIm-BF4, 310% for HMIm-BF4, and 227% for MOIm-BF4, when compared to ammonium acetate at +1000 mV. Different chromatographic methods using the best conditions for each IL were also evaluated and compared. Finally, the best chromatographic conditions using 1 mM of BMIm-BF4 as mobile phase additive, the Nova-Pak® C18 column, 19% (v/v) of acetonitrile content in the mobile phase, and +1000 mV in the ECD, have been applied for the chromatographic analysis of six HAs contained in meat-based infant foods. The whole extraction method of meat-based infant foods using focused microwave-assisted extraction and solid-phase extraction has also been optimized. Extraction efficiencies up to 89% and detection limits ranged between 9.30 and 0.165 ng g−1 have been obtained under optimized conditions. 相似文献