基于实数编码的免疫遗传算法研究

针对标准遗传算法(SGA)搜索效率低、收敛速度慢等缺陷，在免疫遗传算法的基础上提出了基于实数编码的免疫遗传算法(RIGA)。研究表明，RIGA对SGA的改进是有效可行的，显示出稳健的全局优化、计算量少而解的精度高等特点，具有较高的应用价值。     

免疫算法在车辆调度问题中的应用

免疫算法是模仿生物体高度进化、复杂的免疫系统仿生的一种智能化启发式算法。本文根据车辆调度问题的具体情况，应用免疫算法解决车辆调度中路线安排问题，并提出了一种基于分组匹配的亲和力的计算方法。实验结果表明，免疫算法能有效地应用于车辆调度中路线安排问题。     

基于移动Agent技术的医学图像检索方法

针对现有医学图像检索方法中，检索算法速度较慢、准确性较低以及远程检索时占用大量带宽等问题，设计并实现了一种基于移动Agent技术的医学图像检索方法，重点讨论了该方法实现的关键技术，包括提升框架下的M带整数小波变换和提取多通道纹理特征的检索算法．模拟实验结果表明，该方法实时性较强，占用网络带宽较少，采用的算法运算速度提高近10倍、运算复杂度降低约50％，提取的纹理特征能充分表达图像内容，较好地满足了医学图像的检索要求，实现了对医学图像库快速较准确的检索．     

基于多Agent的宏观经济智能预测决策支持系统研究

在分析智能决策支持系统的发展基础上，将人工智能思想，特别是Agent应用于构建智能宏观经济预测决策支持系统。提出面向任务的Agent设计思想，以任务为核心设计多种类型Agent共同完成预测决策任务，给出基于多Agent的系统组成结构，并给出系统中不同类型Agent的具体实现结构，同时探讨了整个系统的运行机制以及系统中多Agent之间的合作。整个系统的构建过程也是面向Agent的程序设计的过程。     

Anti-metatype antibodies in immunoassays

In this review anti-metatype antibodies are described invoking new principles in immunoassay development. Anti-metatype antibodies are immunological reagents specific for the conformation of the liganded antibody active site which do not interact with bound ligand or unliganded antibody. Relationships between anti-metatype antibody reactivity and the ligand-induced conformational state of monoclonal antibodies are reviewed with emphasis on the fluorescein hapten as a small molecule model system. One characteristic result of the interaction of anti-metatype antibodies with liganded antibodies is a significant delay in the dissociation rate (k₂) of the ligand bound within the primary immune complex. The latter is an important consideration for assay development. Polyclonal and monoclonal anti-metatype antibody reagents are characterized in terms of their differential effects on the ligand dissociation rate. Anti-metatype antibody reactivity is further discussed in terms of protein-protein specificity patterns and relative interactions with idiotype-family members, structural derivatives, and site-specific mutants. Incorporation of principles inherent in the anti-metatype concept and their application to assay development are summarized.Abbreviations D₂O deuterium oxide - Fab 50 kd antibody fragment containing V_HC_H1 + V_LC_L domains - FITC(I) fluorescein isothiocyanate (isomer I) - Fv 26 kd fragment of the antibody molecule containing the variable domains of the H and L chains - Ig immunoglobulin - IgG immunoglobulin G with a mol. wt. of 150 kd. - IgM immunoglobulin M with a mol. wt. of 10⁶d - Id idiotype - Ka antibody affinity (k₁/k₂) in M^–1 - k₁ second order rate of ligand association in M^–1s^–1 - k₂ first order rate of ligand dissociation in s^–1 - K_D dissociation constant or the reciprocal of the affinity constant (1/Ka) - Mab monoclonal antibody - Met metatype - NMR nuclear magnetic resonance - SCA single chain Fv derivative containing a synthetic linker between the two variable domains - V_H variable domain of the antibody H chain - V_L variable domain of the antibody L chain     

硒的抗突变性作用与机体免疫功能的研究

研究了在三氯甲烷致突变性试验中加入硒的影响。结果显示硒能减少Ａｍｅｓ试验中三氯甲烷诱变的回变菌落数，能使小鼠骨髓嗜多染红细胞微核率和鳙鱼外周血有核红细胞微核率明显下降。并研究了硒在细胞免疫试验中的作用，结果显示硒能使正常鼠和荷瘤鼠的肺和脾的ＮＫ细胞活性提高，能促进其脾Ｔ淋巴细胞的增殖反应，还能增加外周血Ｔ淋巴细胞数。     

Fluorescence immunological determination of immunoglobulin G in human serum by high-performance liquid gel-permeation chromatography

Summary A high-performance liquid gel-permeation chromatographic method is described for the determination of human serum immunoglobulin G (IgG) by separating the fluorescent immuno complex from the free fluorescence-labeled antibody. Fluorescence-labeled antibody used in this study was fluorescein isothiocyanate (FITC)-labeled Fab fragment goat anti-human IgG (anti-IgG Fab). Immuno complexes and antibody of different molecular sizes can be separated. FITC-labeled anti-IgG Fab was added to the serum and the mixture is passed through the column. An immuno complex separates as well-delineated peak in the column void volume, and was measured by the fluorescence of the column eluate (Ex=490nm, Em=520nm). The total analysis time for a serum sample was approximately 15min. The minimum detection limit was 25 mg/dl. The relative standard deviation was below 2% (peak area). The results of the HPL-GPC analysis correlate well with those obtained by laser nephelometric assay (r=0.992).     

Adaptive Constrained Control of Unknown Strict Feedback Nonlinear Systems With Dead Zone Input北大核心CSCD

研究了具有死区输入的预设约束未知高阶严格反馈非线性系统的控制问题,提出了一种基于免疫函数的自抗扰预设漏斗约束自适应控制策略。首先,针对系统内部的未知问题,采用免疫函数与扩张状态观测器结合对系统内部未知项进行观测;其次,通过Lyapunov方法与漏斗控制相结合设计控制器,使得跟踪误差能够维持在预先设定的漏斗约束范围内;同时,利用双曲正切函数速率变化快这一特性设计自适应控制律,引入指令滤波器避免反步法中重复求导问题,分析证明了闭环系统所有信号的有界性。仿真实例表明了控制方法的有效性。     

Hopf bifurcation of a tumor immune model with time delay

In this paper, a tumor immune model with time delay is studied. First, the stability of nonnegative equilibria is analyzed. Then the time delay τ is selected as a bifurcation parameter and the existence of Hopf bifurcation is proved. Finally, by using the canonical method and the central manifold theory, the criteria for judging the direction and stability of Hopf bifurcation are given.     

An Absence of Non-specific Immune Response towards para-Sulphonato-calix[n]arenes

The effects of para-Sulphonato-calix[4]arene, para-Sulphonato-calix[6]arene and para-Sulphonato-calix[8]arene on the activation of NADPH oxidase in neutrophils has been studied. All three molecules do not induce NADPH oxidase activation, and hence do not stimulate neutrophils. Measurement of cell viability demonstrates that these three water-soluble calix[n]arene derivatives are not cytotoxic.