Extractive Spectrophotometric Determination of Chlorprothixene Hydrochloride

ABSTRACT

A simple, rapid and accurate method for determination of chlorprothixene hydrochloride is presented. It is based on the liability of the chlorprothixene tertiary amine group nitrogen atom to form ion pair complexes with niobium (V) thiocyanate complex. The formed compound is insoluble in water but well soluble in some organic solvents. The reaction is followed spectrophotometrically by measuring the absorbance at λ= 362 nm. This property has been successfully used for the extractive spectrophotometric determination of chlorprothixene. Beer's law was obeyed in the range 9- 50 μg/ml of chlorprothixene hydrochloride. The method is suitable to the determination of analyte in commercial products. The results were compared with those obtained by the official procedure.     

Quantitative High Performance Liquid Chromatographic Determination of Bamipine Combined with Tricyclic Antidepressants and/or Antipsychotics in Pharmaceutical Formulations

Abstract

An advanced reserved phase High Performance Liquid Chromatographic (HPLC) method using UV detection, at 251, is presented for the simultaneous determination of bamipine combined with tricyclic antidepressants and/or antipsychotics in various pharmaceutical formulations. Sample analyses were performed on a bonded reserved-phase C-2,5 μm, 250 × 4.6 mm ID (Lichrosorb RP-2)column using aqueous ammonium acetate at constant ionic strength 0.015 M and acetonitrile 35: 65 as eluent, at a flow rate of 0.9 ml/min. The pH was adjusted to 4.7 with acetic acid. The presence of acetate buffer both shortens the elution time and improves the symmetry of the chromatographic peaks. The retention time was for bamipine, prochlorperazine, trifluoperazine, thioridazine, chlorprothixene, haloperidol, clomipramine, trimipramine 8.83, 11.83, 11.50, 11.08, 10.42, 8.05, 10.25, 9.67 min respectively. Linearity and precision data have been assessed. The method also involves an investigation of the dependence of the capacity ratio k′ on the pH and the polarity of the mobile phase.     

荧光光谱法研究氯普噻吨的氧化反应及其分析应用

氯普噻吨本身不具有荧光 ,但氧化后可形成具有强荧光的产物 .本文系统地研究了以稀硝酸为氧化剂对氯普噻吨的氧化作用 ,提出了高灵敏度测量氯普噻吨的荧光光度分析新方法 .结果表明 :荧光强度与氯普噻吨的浓度在 0 .0 0 4～ 0 .1m g/ L范围内呈良好的线性关系 .该法简便 ,快速 ,灵敏度高 ,检出限可达 0 .0 0 2 mg/ L(6 .33× 10 - 9mol/ L) ,利用该法测量片剂中氯普噻吨的含量 ,结果令人满意 ,平均回收率为 99.6 % .