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A facile approach to assemble catalase-like photosensitizing nanozymes with a self-oxygen-supplying ability was developed. The process involved Fe3+-driven self-assembly of fluorenylmethyloxycarbonyl (Fmoc)-protected amino acids. By adding a zinc(II) phthalocyanine-based photosensitizer (ZnPc) and the hypoxia-inducible factor 1 (HIF-1) inhibitor acriflavine (ACF) during the Fe3+-promoted self-assembly of Fmoc-protected cysteine (Fmoc-Cys), the nanovesicles Fmoc-Cys/Fe@Pc and Fmoc-Cys/Fe@Pc/ACF were prepared, which could be disassembled intracellularly. The released Fe3+ could catalyze the transformation of H2O2 enriched in cancer cells to oxygen efficiently, thereby ameliorating the hypoxic condition and promoting the photosensitizing activity of the released ZnPc. With an additional therapeutic component, Fmoc-Cys/Fe@Pc/ACF exhibited higher in vitro and in vivo photodynamic activities than Fmoc-Cys/Fe@Pc, demonstrating the synergistic effect of ZnPc and ACF.  相似文献   
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Hypoxia is a parameter related to many diseases. Ratiometric hypoxia probes often rely on a combination of an O2-insensitive fluorophore and an O2-sensitive phosphor in a polymer matrix, which require high cost and multi-step synthesis of transition metal complexes. The two-chromophore hypoxia probes encounter unfavorable energy transfer processes and different stabilities of the chromophores. Reported herein is a pure organic ratiometric hypoxia nanoprobe, assembled by a monochromophore, naphthalimide ureidopyrimidinone (BrNpA-UPy), bridged by a bis-UPy-functionalized benzyl skeleton. The joint factors of quadruple hydrogen bonding, the rigid backbone of UPy, and bromine substitution of the naphthalimide derivative facilitate bright phosphorescence (ΦP=7.7 %, τP=3.2 ms) and fluorescence of the resultant nanoparticles (SNPs) at room temperature, which enable accurate, ratiometric, sensitive oxygen detection (Ksv=189.6 kPa−1) in aqueous solution as well as in living HeLa cells.  相似文献   
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荆楠  李创  潘越  钟培峰 《光子学报》2016,(1):102-109
基于探测系统背景辐射特性及高空气球辐射特性,建立了探测系统辐射接收模型.考虑大气传输、光学系统成像、探测器及其采样对辐射的影响,精确计算了高空气球辐射及背景辐射在探测器焦平面阵列上产生的信号电子数,推导出用于高空气球探测的信噪比.利用Modtran软件仿真计算了自身辐射、镜背景辐射、漫背景辐射亮度,分析了复杂大气条件下的气球辐射特性,及高空气球镜反射率、漫反射率与积分时间对探测系统信噪比的影响.结果表明:采用光谱滤波技术,在晴朗无云天气下,可见光近红外(0.6~2.4)探测器适合高空气球探测;在复杂大气条件下,长波红外(8~12)探测器适合高空气球探测;在积分时间为0.25s,镜反射率为0.32,漫反射率为0.68时,或积分时间为1s,镜反射率0.43,漫反射率0.57时,探测系统对高空气球探测能力最强.  相似文献   
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Raman spectroscopy is an advanced chemical analytical technique that has gained significant interest in cancer research, in particular early detection and monitoring of cancer, with added advantages of non-invasive and real-time diagnosis. Recently, studies have shown its sensitivity to monitor chemical changes during cancer progression. This information will lead to identification of chemical markers (molecular fingerprints of chemical composition) that can be used as biological markers. In this study, we used a tumor spheroid model that mimics the characteristics of a non-vascular in vitro tumor model, we used a combination of Raman and multivariate approach to identify chemical changes associated with normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroid model. The results provide evidence that lipids, amide I, III and nucleic acid contents differ significantly in normal, hypoxic and necrotic regions. Principal component analysis loading plots has suggested that normal proliferating region separated with low amide I and high-tryptophan content compared to hypoxic and necrotic regions. These differences observed in three regions might be useful in identification of new spectral markers associated stress faced by each region progressing toward necrosis.  相似文献   
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It is of engineering importance to locate low altitude moving targets with acoustic methods due to the blindness of the traditional radar detecting. In this paper, an algorithm for locating low altitude moving targets is put forward based on a five-element planar acoustic sensor array. The method is realized through estimations of the sensor-to-sensor time delays of the source signal generated by the low altitude moving target. The angle and range estimation performance of the proposed method are analyzed, respectively, both in theoretical and numerical sense.  相似文献   
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开都河——孔雀河流域水资源综合利用优化管理模型   总被引:1,自引:0,他引:1  
根据开都河——孔雀河流域水资源系统的特点 ,从系统分析的角度出发 ,综合考虑水利 ,水电及生态环境保护工程效益 ,提出了一个多目标优化管理模型 .该模型能模拟实际系统的运行 ,还具有良好的扩展性 ,为科学治理保护开都河——孔雀河流域提供了可靠的科学依据 .  相似文献   
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A new network has been recently proposed for the provision of fixed broadband services via high altitude platform stations (HAPS) in the 48/47 GHz band. One of the critical issues of this technology is spectrum sharing with the fixed satellite service. This paper examines the effect of Earth-to-stratosphere co-channel interference produced by ground stations of HAPS network to geostationary orbit (GEO) satellite receivers and proposes an interference-to-noise (I/N) calculation model for the evaluation of the current HAPS ground stations power characteristics set by ITU-R. Analytical and simulation results for archetypal systems in realistic scenarios indicate that efficient use of the spectrum shared between heterogeneous HAPS and Fixed Satellite Networks is feasible. Certain consideration is needed prior to the HAPS network development in suburban areas for latitudes from 55° to 65°, and in rural areas for latitudes from 60° to 75°.  相似文献   
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Obtained benzimidazole derivatives, our newly synthesized heterocyclic compounds, belong to a new group of chemical bondings with potential anticancer features. A biochemical study determining anticancer activity of benzimidazole derivatives was conducted in the environment of 0.2% DMSO (dimethyl sulfoxide). The HPLC method was used to determine stability of these compounds in a biochemical study environment. Optimization of the chromatographic system and validation of the established analytical method were performed. Stability analysis of the derivatives was examined in the reversed phases RP-8 system, where pure acetonitrile was used as an eluent for the analysis of compound 14. The HPLC analysis revealed that the compounds studied are homogeneous in the environment of 0.2% DMSO up to 96 hr.  相似文献   
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Despite its clinical promise, photodynamic therapy (PDT) suffers from a key drawback associated with its oxygen‐dependent nature, which limits its effective use against hypoxic tumors. Moreover, both PDT‐mediated oxygen consumption and microvascular damage further increase tumor hypoxia and, thus, impede therapeutic outcomes. In recent years, numerous investigations have focused on strategies for overcoming this drawback of PDT. These efforts, which are summarized in this review, have produced many innovative methods to avoid the limits of PDT associated with hypoxia.  相似文献   
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