排序方式: 共有3条查询结果,搜索用时 0 毫秒
1
1.
Qiang Wang~a Jian Wu Wang~b Zheng Ting Cai~ 《中国化学快报》2008,19(4):497-500
To explore the closing mechanism of the substrate pocket,we perform a 16,000 ps molecular dynamics simulation separately on the ligand-free and actinonin-bound peptide deformylase from Leptospira interrogans.Our results show that the CD-loop, hydrophilic inhibitor and hydrophobic cluster are necessary for the formation of semi-open conformation,and Tyr71 plays an important role in mediating the movements of CD-loop.The average MD structure of the actinonin-bound LiPDF complex approaches to the crystal structure.These are consistent with experiment very well. 相似文献
2.
钩端螺旋体赖型赖株(L.interrogansSerovarlai)DNA分别经BamHI,HindⅢ和PstⅠ3种内切酶酶切完全消化,通过设计1对引物,反向PCR扩增问号赖型钩体重组质粒pDJH2插入片段两端的未知序列.结果显示不同酶切环化连接,经反向PCR扩增均可得2条大小相等的扩增带.结果提示反向PCR技术可以用于问号赖型钩体重组质粒插入片段两端的未知序列的研究,为获得钩体完整基因序列提供新的途径. 相似文献
3.
1