液相色谱法同时测定维药蜀葵花中芦丁、槲皮素和山柰酚

维药是祖国医药学不可分割的组成部分。维药现代化,即利用现代技术研究维药的有效成分,是维药科学化、标准化、规范化、商品化和产业化的必经之路。本文建立了维药蜀葵花中有效成分芦丁、槲皮素和山柰酚的选择性提取方法,优化了高效液相色谱法(HPLC)同时测定这3种有效成分的分析条件。采用HC-C₁₈色谱柱(250 mm×4.6 mm, 5 μm)和甲醇-0.4%磷酸(50:50, v/v)流动相,在柱温30 ℃和流速1.00 mL/min的条件下实现了3种物质之间以及和干扰物之间的基线分离。维药蜀葵花中芦丁、槲皮素及山柰酚的线性范围分别为12.5~150 μg/mL (r=0.9998), 12.5~125 μg/mL (r=0.9999)及12.5~125 μg/mL (r=0.9988),加标回收率(n=5)分别为100.3%(RSD=1.1%)、97.60%(RSD=0.47%)、97.75%(RSD=0.71%)。该方法实现了同时测定维药蜀葵花中芦丁、槲皮素及山柰酚,为其他黄酮类物质的开发应用提供了科学依据,同时也可为其他维药分析提供借鉴。     

Lipids and Carbohydrates from Althaea nudiflora and A. armeniaca Roots

Lipids from roots ofAlthaea nudifloraandA. Armeniacawere studied. The carbohydrate and fatty-acid compositions were found. The lipids ofA. NudifloraandA. Armeniacacontain 22.3 and 12.6%, respectively, of cyclopropenoid fatty acids, the physical chemical properties of which are presented. The optimal degree of grinding, temperature, and raw-material-to-extractant ratio for aqueous extraction of water-soluble polysaccharides from althaea roots were chosen from a single statistically significant experiment     

蜀葵花紫色素的提取及其稳定性研究

从蜀葵花瓣中提取的紫色素是一种安全无害的天然染料,本文对蜀葵花中的紫色素的提取及稳定性的研究作了介绍。