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1.
Grainger Reid J. Ko Samuel Koslov Eugene Prokop Ales Tanner Robert D. Loha Veara 《Applied biochemistry and biotechnology》2000,84(1-9):761-768
Human insulin in zinc suspension was used as a model protein to test the effect of shear on the settling rate of proteins,
a possible inference for protein denaturation. The rate of settling was determined directly in a spectropho-tometer. Shear
effects are important in retaining the activity of proteins and are present in bubble, foam, and droplet protein fractionation
processes. A sim pletest, such as that conducted here, mayeven be useful for monitoring changes in protein structure caused
by commercial shipping of the protein. The settling ratefor insulin was continously monitored in theoriginal bottle by spectrophotometric
absorbance changes as a function of time. A settling curve was determined following each shear experiment, which included
shaking the “worked” insulin solution in a vortex mixer for different lengths of time. It was determined, when comparing long
shaking times with short ones, that the initial settling rate was less for the long-term shaking of the insulin samples and
greater for the short-term shaking. The secondary effects of light and heat, along with shaking, a pparently did not produce
differences from shaking alone. 相似文献
2.
A sphere in air will roll down a plane that is tilted away from the vertical. The only couple acting about the point of contact between the sphere and the plane is due to the component of the weight of the sphere along the plane, provided that air friction is negligible. If on the other hand the sphere is immersed in a liquid, hydrodynamic forces will enter into the couples that turn the sphere, and the rotation of the sphere can be anomalous, i.e., as if rolling up the plane while it falls. In this paper we shall show that anomalous rolling is a characteristic phenomenon that can be observed in every viscoelastic liquid tested so far. Anomalous rolling is normal for hydrodynamically levitated spheres, both in Newtonian and viscoelastic liquids. Normal and anomalous rolling are different names for dry and hydrodynamic rolling. Spheres dropped at a vertical wall in Newtonian liquids are forced into anomalous rotation and are pushed away from the wall while in viscoelastic liquids, they are forced into anomalous rotation, but are pushed toward the wall. If the wall is inclined and the fluid is Newtonian, the spheres will rotate normally for dry rolling, but the same spheres rotate anomalously in viscoelastic liquids when the angle of inclination from the vertical is less than some critical value. The hydrodynamic mechanisms underway in the settling of circular particles in a Newtonian fluid at a vertical wall are revealed by an exact numerical simulation based on a finite-element solution of the Navier-Stokes equations and Newton's equations of motion for a rigid body. 相似文献
3.
Two membrane bioreactors (MBRs; volume = 300 L) equipped with different types of immersed membrane modules were operated simultaneously
under the same laboratory conditions as a low-loaded activated sludge process without any membrane regeneration and excess
sludge uptake (sludge retention time SRT up to 170 d; activated sludge concentration MLSS up to 11 g L−1). The aim was to verify the quality of treated water and to study the properties of "very old" activated sludge. Another
aim was to compare different selected membrane types and choose the best one for further pilot-scale testing.
Presented at the 35th International Conference of the Slovak Society of Chemical Engineering, Tatranské Matliare, 26–30 May
2008. 相似文献
4.
针对中大规模红外焦平面对高速读出的需求,研究并设计了一款20MPixel/s红外焦平面高速读出电路。读出电路单元电路由电容负反馈运放输入级、相关双采样、源随输出级电路组成,总线输出级采用基于低功耗推挽运放的跟随器结构。研究了输出级运放像元信号建立时间和负载电容的关系,给出了20 MPixel/s高速读出的负载电容适用范围。采用0.5μm Mixed Signal CMOS工艺研制了一款红外焦平面高速读出电路芯片,和InGaAs光敏芯片耦合后实测读出速率达到20MPixel/s,像元信号之间最大上升时间为17ns。 相似文献
5.
基于Mie散射理论和多重散射理论探讨了亚波长介质柱阵列对电磁波的调控. 研究结果表明: 当在全反射的单层介质柱阵列中引入一个空位缺陷时会产生12%的透射; 如果在入射一侧再引入一合适的介质柱时, 其透射率可增加至36%, 为空位缺陷时的3倍; 当在出射一侧对称位置处引入另一完全相同的介质柱时, 可以调制透射电磁波的模式, 虽然总的透射率没有增加,但向前散射的电磁波能量明显增强. 采用这种双粒子耦合体系, 在金属柱的表面等离激元共振频率附近也可以实现类似的效果. 这些体系结构简单、易于在实验上实现, 这对于太赫兹甚至光频段的光子集成线路中的元件设计和光束调控很有意义. 相似文献
6.
Finite element solutions are presented for the flow of Newtonian and non-Newtonian fluids around a sphere falling along the centreline of a cylindrical tube. Both rotating and stationary tube scenarios are considered. Calculations are reported for three different inelastic constitutive models that manifest shear-thinning, extension-thickening and their combination. The influence of inertia and these various forms of viscous response are examined for their influence upon the drag on the settling particle and the structure of the flow. Simulations are performed by employing a semi-implicit time marching Taylor–Galerkin/pressure-correction finite element algorithm, a fractional-staged scheme of second-order-accuracy. © 1998 John Wiley & Sons, Ltd. 相似文献
7.
Three test problems were simulated using five different two‐phase flow model equation sets from the open literature. The test problems chosen were a fluidized bed, a batch settling, and a horizontal jet impingement on a vertical wall. These three problems demonstrate an important cross‐section of physical phenomena, such as fluidized bed voidage oscillations, phase separation, countercurrent flow, and jet formation. The dispersed flow regime is selected for all three problems. The study was performed to assess the basic character of the five‐field equation sets responding to the same initial and boundary conditions and using the same finite difference numerical scheme. The general performance of the five equation sets was found to be similar, even though one of them was ill posed as an initial‐value problem. Broad trends are the same and quantitative differences could be assessed by examining the fine structure of the results. None of the equation sets could be entirely rejected on the basis of producing physically impossible or unacceptable results. Copyright © 2000 John Wiley & Sons, Ltd. 相似文献
8.
9.
This paper presents a theoretical model for computing the velocity and sediment concentration profiles in a uniform sediment-laden flow carrying all fine, medium and coarse sediments. The proposed model essentially includes the effect of sediment concentration in total turbulent shear stress and eddy diffusivity in addition to the modified mixing length derived by Umeyama and Gerritsen [J. Hydr. Engng., ASCE, 118 (2) (1992) 229–245] applied to Hunt’s diffusion equation. Numerical solution of coupled differential equations for velocity and sediment concentration is carried out. The theoretical results show quite good agreement with the experimental data. 相似文献
10.
As a first step towards understanding particle–particle interaction in fluid flows, the motion of two spherical particles settling in close proximity under gravity in Newtonian fluids was investigated experimentally for particle Reynolds numbers ranging from 0.01 to 2000. It was observed that particles repel each other for Re>0.1 and that the separation distance of settling particles is Reynolds number dependent. At lower Reynolds numbers, i.e. for Re<0.1, particles settling under gravity do not separate.The orientation preference of two spherical particles was found to be Reynolds number dependent. At higher Reynolds numbers, the line connecting the centres of the two particles is always horizontal, regardless of the way the two particles are launched. At lower Reynolds numbers, however, the particle centreline tends to tilt to an arbitrary angle, even of the two particles are launched in the horizontal plane. Because of the tilt, a side migration of the two particles was found to exist. A linear theory was developed to estimate the side migration velocity. It was found that the maximum side migration velocity is approximately 6% of the vertical settling velocity, in good agreement with the experimental results.Counter-rotating spinning of the two particles was observed and measured in the range of Re=0–10. Using the linear model, it is possible to estimate the influence of the tilt angle on the rate of rotation at low Reynolds numbers. Dual particles settle faster than a single particle at small Reynolds numbers but not at higher Reynolds numbers, because of particle separation. The variation of particle settling velocity with Reynolds number is presented. An equation which can be used to estimate the influence of tilt angle on particle settling velocity at low Reynolds number is also derived. 相似文献