Integrated chemical sensor array platform based on a light emitting diode, xerogel-derived sensor elements, and high-speed pin printing

We report a new, solid-state, integrated optical array sensor platform. By using pin printing technology in concert with sol-gel-processing methods, we form discrete xerogel-based microsensor elements that are on the order of 100 μm in diameter and 1 μm thick directly on the face of a light emitting diode (LED). The LED serves as the light source to excite chemically responsive luminophores sequestered within the doped xerogel microsensors and the analyte-dependent emission from within the doped xerogel is detected with a charge coupled device (CCD). We overcome the problem of background illumination from the LED reaching the CCD and the associated biasing that results by coating the LED first with a thin layer of blue paint. The thin paint layer serves as an optical filter, knocking out the LEDs red-edge spectral tail. The problem of the spatially-dependent fluence across the LED face is solved entirely by performing ratiometric measurements. We illustrate the performance of the new sensor scheme by forming an array of 100 discrete O₂-responsive sensing elements on the face of a single LED. The combination of pin printing with an integrated sensor and light source platform results in a rapid method of forming (∼1 s per sensor element) reusable sensor arrays. The entire sensor array can be calibrated using just one sensor element. Array-to-array reproducibly is <8%. Arrays can be formed using single or multiple pins with indistinguishable analytical performance.     

Study on the Collapse of Pin-Reinforced Foam Sandwich Panel Cores

New fabrication technologies now allow for hybrid sandwich structures, known as X-core, to be manufactured. The X-core panels consist of a pin reinforced polymer foam core with carbon fiber face sheets. Carbon fiber or metallic (Titanium/Steel) pins are inserted into the foam core in the out-of-plane direction and extend from face sheet to face sheet. The through thickness three-point simply supported bending behavior of these panels is used to evaluate the collapse characteristics of the panels. Explicit experimental observations are used to calibrate analytical energy balance models describing the panel collapse as a function of geometry and properties. The mechanical response of X-core sandwich panels is compared to current sandwich materials for material selection.     

HL-2A先进二维硬X射线成像CCD系统设计

为HL-2A装置设计了一种二维硬X射线成像CCD系统,用它能直接测量硬X射线辐射强度的二维时空分布I(z,y,t),并能得到电子速率分布fe(v,r,t)。为获得二级加热和无感电流驱动如ECH／ECCD,LHH／LHCD,ICRH／ICCD,NBIH／NBICD的效果、波可近性、共振层位置、能量沉积范围等方面的实验研究提供重要诊断手段;也可用于内破裂、大破裂、MHD,特别是q=2附近高模的MHD行为、热电子和超热电子的输运、被捕获的高能粒子图像以及与模式波(如m=1,n=1)共振引起的鱼骨模不稳定性、辅助加料引起和诱发逃逸电子雪崩现象的实验观察与研究。     

Behavior of Pin-loaded Laminated Composites

In this study, an investigation was carried out to determine the effects of joint geometry and fiber orientation on the failure strength and failure mode in a pinned joint laminated composite plate. Behavior of pin-loaded laminated composites with different stacking sequence and different dimensions has been observed experimentally. E/glass–epoxy composites were manufactured to fabricate the specimens. Mechanical properties of the composites were characterized under tension, compression and in-plane shear in static loading conditions. Laminated composites were loaded through pins. Single-hole pin-loaded specimens were tested for their tensile response and width-to-hole diameter (W/D) and edge distance-to-hole diameter (E/D) ratios evaluated. A series of experiments was performed with six different material configurations ([0/±45]_s–[90/±45]_s, [0/90/0]_s–[90/0/90]_s and [90/0]_2s–[±45]_2s), in all, over 120 specimens. E/D ratios and W/D ratios of plates were changed from 1 to 5 and 2 to 5, respectively. Failure propagation and failure type were observed on the specimens. The influence of the joint geometry on the strength of the pin-loaded composites was assessed. When laminated composite plates were loaded to final failure, three basic failure modes consisting of net-tension, shear out and bearing failure were observed for the different geometric dimensions. All the connections tested showed that the fiber orientations have a definite influence on the position around hole circumference at which failure initiated. Net-tension failure occurred for specimens that had small width and large end distance. When the width was increased, the specimens which had small end distances failed in the shear-out modes. When the end distance was increased, bearing failure developed in addition to shear-out failure. The experimental results showed that the ultimate load capacities of E/glass–epoxy laminate plates with pin connection were increased by increasing W and E. However, increasing the E/D and W/D ratios beyond a critical value has an insignificant effect on the ultimate load capacity of the connection.     

Multi-objective optimization of pin fin to determine the optimal fin geometry using genetic algorithm

In this study, one dimensional heat transfer in a pin fin is modeled and optimized. We used Bezier curves to determine the best geometry of the fin. The model equations are solved to analyze the heat transfer. Total heat transfer rate and fin efficiency factor are considered as two objective functions and multi-objective optimization carried out to maximize heat transfer rate and fin efficiency simultaneously. Fast and elitist non-dominated sorting genetic algorithm (NSGA-II) is used to determine a set of multiple optimum solutions, called ‘Pareto optimal solutions. The optimized results are presented with Pareto front which demonstrate conflict between two objective functions in the optimized point, both energy conservation and thermal analysis are carried out to verify the solution method and the results shows good precision.     

Discovery of a Prenylated Flavonol Derivative as a Pin1 Inhibitor to Suppress Hepatocellular Carcinoma by Modulating MicroRNA Biogenesis

Peptidyl‐prolyl cis‐trans isomerase Pin1 plays a crucial role in the development of human cancers. Recently, we have disclosed that Pin1 regulates the biogenesis of miRNA, which is aberrantly expressed in HCC and promotes HCC progression, indicating the therapeutic role of Pin1 in HCC therapy. Here, 7‐(benzyloxy)‐3,5‐dihydroxy‐2‐(4‐methoxyphenyl)‐8‐(3‐methylbut‐2‐en‐1‐yl)‐4H‐chromen‐4‐one ( AF‐39 ) was identified as a novel Pin1 inhibitor. Biochemical tests indicate that AF‐39 potently inhibits Pin1 activity with an IC₅₀ values of 1.008 μm , and also displays high selectivity for Pin1 among peptidyl prolyl isomerases. Furthermore, AF‐39 significantly suppresses cell proliferation of HCC cells in a dose‐ and time‐dependent manner. Mechanistically, AF‐39 regulates the subcellular distribution of XPO5 and increases miRNAs biogenesis in HCC cells. This work provides a promising lead compound for HCC treatment, highlighting the therapeutic potential of miRNA‐based therapy against human cancer.     

Direct 13C-detection for carbonyl relaxation studies of protein dynamics

We describe a method that uses direct ¹³C-detection for measuring rotating-frame carbonyl (¹³CO) relaxation rates to describe protein functional dynamics. Key advantages of method include the following: (i) unique access to ¹³CO groups that lack a scalar-coupled ¹⁵N–¹H group; (ii) insensitivity to ¹⁵N/¹H exchange-broadening that can derail ¹H-detected ¹⁵N and HNCO methods; (iii) avoidance of artifacts caused by incomplete water suppression. We demonstrate the approach for both backbone and side-chain ¹³CO groups. Accuracy of the ¹³C-detected results is supported by their agreement with those obtained from established HNCO-based approaches. Critically, we show that the ¹³C-detection approach provides access to the ¹³CO groups of functionally important residues that are invisible via ¹H-detected HNCO methods because of exchange-broadening. Hence, the ¹³C-based method fills gaps inherent in canonical ¹H-detected relaxation experiments, and thus provides a novel complementary tool for NMR studies of biomolecular flexibility.     

不同浸润性多孔电极表面的气泡行为

采用氢气模板法制备了具有多孔结构的电极; 通过改变电镀电流密度和电镀时间实现了电极表面多孔结构孔径和分布的控制; 通过改变表面化学组成有效调控了电极表面的浸润性质. 比较了具有不同微观结构和表面化学组成的电极在给定条件下电解水过程中气泡的产生及行为机制. 实验结果表明: 相对于亲水的多孔电极, 疏水的多孔电极表面能够黏附气泡, 更易倾向于形成稳定的气膜; 多孔结构对于亲水电极表面气泡行为的影响比对疏水电极表面气泡行为的影响更为显著; 与没有多孔结构的亲水电极相比, 具有多孔结构的亲水电极表面产生的气泡数量多, 速率快; 与较小孔径的多孔亲水电极相比, 较大孔径的多孔亲水电极表面产生气泡速率快且黏附气泡数量少. 该研究结果为微气泡减阻电极的设计提供了理论依据.     

Distribution of Friction Heat Between a Stationary Pin and Rotating Ring

The analysis of the heat distribution between a stationary pin and rotating ring was considered. To solution of the governing quasi-stationary heat conductivity equation the finite Fourier transform was used. The convective cooling from outer and internal surface of the ring as the boundary conditions were considered. The ring surface temperature, the average temperature on the ring surface and the heat distribution coefficient for the studied system were determined. The numerical results for the temperatures and heat distribution coefficient which demonstrated the effects of the Biot number and internal radius of the ring on them, were presented.