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1.
Dr. Joaquim Torra Dr. Felipe Viela Dr. Diego Megías Dr. Begoña Sot Prof. Cristina Flors 《Chemistry (Weinheim an der Bergstrasse, Germany)》2022,28(19):e202200026
CRANAD-2 is a fluorogenic curcumin derivative used for near-infrared detection and imaging in vivo of amyloid aggregates, which are involved in neurodegenerative diseases. We explore the performance of CRANAD-2 in two super-resolution imaging techniques, namely stimulated emission depletion (STED) and single-molecule localization microscopy (SMLM), with markedly different fluorophore requirements. By conveniently adapting the concentration of CRANAD-2, which transiently binds to amyloid fibrils, we show that it performs well in both techniques, achieving a resolution in the range of 45–55 nm. Correlation of SMLM with atomic force microscopy (AFM) validates the resolution of fine features in the reconstructed super-resolved image. The good performance and versatility of CRANAD-2 provides a powerful tool for near-infrared nanoscopic imaging of amyloids in vitro and in vivo. 相似文献
2.
Piotr Garbaczewski 《Journal of statistical physics》2006,123(2):315-355
We analyze the functioning of Gibbs-type entropy functionals in the time domain, with emphasis on Shannon and Kullback-Leibler entropies of time-dependent continuous probability distributions. The Shannon entropy validity is extended to probability distributions inferred from L
2(R
n
) quantum wave packets. In contrast to the von Neumann entropy which simply vanishes on pure states, the differential entropy quantifies the degree of probability (de)localization and its time development. The associated dynamics of the Fisher information functional quantifies nontrivial power transfer processes in the mean, both in dissipative and quantum mechanical cases.
PACS NUMBERS: 05.45.+b, 02.50.-r, 03.65.Ta, 03.67.-a 相似文献
3.
An experiment was designed to determine whether normally sighted human subjects would be able to adapt to the handicapping effects of sudden deprivation of visual cues on horizontal plane sound localization. Two groups of sighted normal-hearing young adults participated. One group was allowed the benefit of sight. The other group was blindfolded. Measurements of accuracy and the time to respond were made daily over the course of five consecutive days, in a semi-reverberant sound proof booth that modeled listening in a small office. Sound localization was assessed using an array of eight speakers that surrounded the subject in space. Each day, one block of 120 trials was presented for each of three stimuli, two one-third octave noise bands, centred at 0.5 and 4 kHz, and broadband noise, to assess the utilization of interaural temporal difference cues, interaural level difference cues and binaural and spectral cues in combination. Blindfolded subjects were relatively less accurate than sighted subjects. Both groups showed gains with practice, the blindfolded group to a greater degree, largely due to improvements in the use of spectral cues. The blindfolded group took longer to respond than the sighted group, but showed greater decrements in response time with practice. 相似文献
4.
5.
新型X射线靶设计为:由SiO2和TiO2组成具有12个周期的一维光子晶体,在它的中间嵌入光靶材料层作为缺陷层,SiO2,TiO2和光靶层的光学厚度分别为λ4、λ4和λ2,λ为抽运激光波长.与普通平板光靶相比,当抽运光垂直照射到这种光靶时,靶层内部的光强将提高2个数量级,所以抽运激光的阈值强度将降低2个数量级,这有利于X射线激光的小型化.在同样的抽运激光照射下,X射线激光的强度将提高4个数量级,转换效率也将提高约4个数量级.由于平均电离度随抽运激光强度的提高而提高,所以采用这种光靶有利于使X射线激光向短波长推进.
关键词:
X射线激光
光子晶体
光波局域 相似文献
6.
T.M. Mishonov N. Chéenne D. Robbes J.O. Indekeu 《The European Physical Journal B - Condensed Matter and Complex Systems》2002,26(3):291-296
The generation of harmonics of the voltage response is considered when an AC current is applied through a superconducting
film above Tc. It is shown that almost at all temperatures the mechanism of the temperature oscillations created by the AC current and
the temperature dependence of the resistance dominates over the isothermal nonlinear electric conductivity. Only in a narrow
critical region close to Tc the latter is essential for the generation of the harmonics. A detailed investigation of harmonics generation provides an
accurate method for measuring the thermal boundary conductance between the film and the insulating substrate. The critical
behaviour of the third harmonic will give a new method for the determination of the lifetime of metastable Cooper pairs above
Tc. The comparison of the calculated fifth harmonics of the voltage with the experiment is proposed as an important test for
the applicability of the employed theoretical models.
Received 8 September 2001 相似文献
7.
In this paper, an adaptive FE analysis is presented based on error estimation, adaptive mesh refinement and data transfer for enriched plasticity continua in the modelling of strain localization. As the classical continuum models suffer from pathological mesh-dependence in the strain softening models, the governing equations are regularized by adding rotational degrees-of-freedom to the conventional degrees-of-freedom. Adaptive strategy using element elongation is applied to compute the distribution of required element size using the estimated error distribution. Once a new mesh is generated, state variables and history-dependent variables are mapped from the old finite element mesh to the new one. In order to transfer the history-dependent variables from the old to new mesh, the values of internal variables available at Gauss point are first projected at nodes of old mesh, then the values of the old nodes are transferred to the nodes of new mesh and finally, the values at Gauss points of new elements are determined with respect to nodal values of the new mesh. Finally, the efficiency of the proposed model and computational algorithms is demonstrated by several numerical examples. 相似文献
8.
Elias Zafiris 《International Journal of Theoretical Physics》2004,43(1):265-298
We develop a relativistic perspective on structures of quantum observables, in terms of localization systems of Boolean coordinatizing charts. This perspective implies that the quantum world is comprehended via Boolean reference frames for measurement of observables, pasted together along their overlaps. The scheme is formalized categorically, as an instance of the adjunction concept. The latter is used as a framework for the specification of a categorical equivalence signifying an invariance in the translational code of communication between Boolean localizing contexts and quantum systems. Aspects of the scheme semantics are discussed in relation to logic. The interpretation of coordinatizing localization systems, as structure sheaves, provides the basis for the development of an algebraic differential geometric machinery suited to the quantum regime. 相似文献
9.
T. Mihara K. Miyamoto M. Kida T. Sasaki N. Aoki Y. Ochiai 《Superlattices and Microstructures》2003,34(3-6):383
A multiwall carbon nanotube crossroads has been fabricated by a manipulation technique using a glass microcapillary, and the low temperature transport properties investigated. The two-terminal conductance of an individual tube shows Tomonaga–Luttinger liquid behavior G∝Tα at high temperature and dI/dV ∝V α at low temperature. However, no evidence of such a power-law behavior is obtained in the four-terminal conductance at the junction, where the conductance shows an almost metallic behavior ‘corrected’ by weak localization. Weak localization would essentially appear in electron states at the junctions of MWNTs. 相似文献
10.
In this note we make a correction in the paper [1] by two of the authors. Specifically we modify an auxiliary localization
result and prove that the applications to the saturation theory of some outstanding conservative operators remain valid. Moreover,
we enrich a corollary dealing with the well-known Szász-Mirakjan operators.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献